P23046 · NSP5_ROTBV

Function

function

Plays an essential role in the viral genome replication. Participates, together with NSP2, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication intermediates are assembled and viral RNA replication takes place. Orchestrates the recruitment of viroplasmic proteins such as capsid proteins to these factories. Participates in the selective exclusion of host proteins from stress granules (SG) and P bodies (PB). Participates also in the sequestration of these remodeled organelles in viral factories.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

119820406080100120140160180
TypeIDPosition(s)Description
Binding site92Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell cytoplasm
Molecular FunctionATP hydrolysis activity
Molecular Functionmagnesium ion binding
Molecular Functionnucleotide binding
Molecular FunctionRNA binding
Biological Processviral genome replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Non-structural protein 5
  • Short names
    NSP5
  • Alternative names
    • NS26

Organism names

Accessions

  • Primary accession
    P23046

Subcellular Location

Host cytoplasm
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001496321-198Non-structural protein 5
Modified residue67Phosphoserine; by host CK1
Modified residue154Phosphoserine; by host
Modified residue156Phosphoserine; by host
Modified residue164Phosphoserine; by host
Modified residue166Phosphoserine; by host

Post-translational modification

O-glycosylated.
Hyperphosphorylated on serine residues, when in dimeric form. Phosphorylation by host CK1 is required for the hyperphosphorylation of NSP5 dimer.

Keywords

Interaction

Subunit

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6. Participates in the selective exclusion of host proteins from stress granules (SG) and P bodies (PB). Participates also in the sequestration of these remodeled organelles in viral factories.

Structure

3D structure databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region127-167Disordered

Sequence similarities

Belongs to the rotavirus NSP5 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    198
  • Mass (Da)
    21,679
  • Last updated
    1991-11-01 v1
  • Checksum
    28B8F0095A7C4595
MSLSIDVTSLPSFSSSIYKNESSATASTLSGKSIGRSVQYVSPDAEAFSKYMLSKSPEDIGPSDSASNDPLTSFSIRSNAVKTNADAGVSMDSSVQSRPSINVGCDQVDFSFNKGIKVNANLDSSISVSTNSRKEKSKGDRKSRKHYPKIEAESDSDEYVLDDSDSDDGKCRNCKYKRKYFALRMRMKQVAMQLIEDL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M33606
EMBL· GenBank· DDBJ
AAA47314.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp