P22953 · HS701_ARATH
- ProteinHeat shock 70 kDa protein 1
- GeneHSP70-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids651 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
In cooperation with other chaperones, Hsp70s are key components that facilitate folding of de novo synthesized proteins, assist translocation of precursor proteins into organelles, and are responsible for degradation of damaged protein under stress conditions (Probable). Probably involved in defense response. Chaperone involved in protein targeting to chloroplasts. May cooperate with SGT1 and HSP90 in R gene-mediated resistance towards the oomycete Hyaloperonospora parasitica (downy mildew). Plays a role with WPP-domain proteins in facilitating WIT1 nuclear envelope targeting (PubMed:12805626, PubMed:18065690, PubMed:19617588).
Modulates stomatal aperture in response to various environmental conditions and physiological responses to the hormone abscisic acid (ABA) (PubMed:21586649).
Modulates stomatal aperture in response to various environmental conditions and physiological responses to the hormone abscisic acid (ABA) (PubMed:21586649).
Miscellaneous
Plants overexpressing HSP70-1 show disabled immune responses, enhanced tolerance to heat shock and altered plant growth and development (PubMed:12805626).
Plants overexpressing HSP70-1 are hypersensitive to abscisic acid (ABA) in seed germination assays, and are compromised in the dark- and ABA-induced stomatal closure (PubMed:21586649).
Plants overexpressing HSP70-1 are hypersensitive to abscisic acid (ABA) in seed germination assays, and are compromised in the dark- and ABA-induced stomatal closure (PubMed:21586649).
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | apoplast | |
Cellular Component | chloroplast | |
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | cytosolic ribosome | |
Cellular Component | Golgi apparatus | |
Cellular Component | nucleolus | |
Cellular Component | nucleus | |
Cellular Component | plant-type cell wall | |
Cellular Component | plant-type vacuole | |
Cellular Component | plasma membrane | |
Cellular Component | plasmodesma | |
Molecular Function | ATP binding | |
Molecular Function | ATP-dependent protein folding chaperone | |
Molecular Function | mRNA binding | |
Molecular Function | protease binding | |
Biological Process | defense response to bacterium | |
Biological Process | defense response to fungus | |
Biological Process | defense response to other organism | |
Biological Process | heat acclimation | |
Biological Process | negative regulation of seed germination | |
Biological Process | response to heat | |
Biological Process | response to virus | |
Biological Process | stomatal closure |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameHeat shock 70 kDa protein 1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP22953
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth conditions (PubMed:21418353, PubMed:26408532, PubMed:28004282).
The double mutants hsp70-1 and hsp70-4 exhibit accelerated bolting, flowering, and branching, small round shape and flat leaf blades, thin stems and short siliques (PubMed:28004282).
The double mutants hsp70-1 and hsp70-4 exhibit reduced tolerance to abiotic stresses, such as heat, cold, salt and osmotic shock (PubMed:28004282).
The double mutants hsp70-1 and hsp70-4 exhibit accelerated bolting, flowering, and branching, small round shape and flat leaf blades, thin stems and short siliques (PubMed:28004282).
The double mutants hsp70-1 and hsp70-4 exhibit reduced tolerance to abiotic stresses, such as heat, cold, salt and osmotic shock (PubMed:28004282).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000078344 | 2-651 | Heat shock 70 kDa protein 1 | |||
Sequence: SGKGEGPAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPVNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQAGPADKPMIYVEYKGEEKEFAAEEISSMVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGNEKVQDLLLLDVTPLSLGLETAGGVMTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMVQEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEFEDKMKELESICNPIIAKMYQGAGGEAGGPGASGMDDDAPPASGGAGPKIEEVD |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed at a substantial level during normal growth in root, stem, leaf and flower tissues, but not in siliques (PubMed:8049382).
Expressed in cotyledons, leaves, stems, vascular bundles, roots, stigmas and anthers (PubMed:28004282).
Expressed in cotyledons, leaves, stems, vascular bundles, roots, stigmas and anthers (PubMed:28004282).
Induction
Induced by heat shock and cold (PubMed:11402207).
Induced by dehydration stress and abscisic acid (ABA) (PubMed:8075396).
Up-regulated by viral infection (PubMed:15805473).
Induced by dehydration stress and abscisic acid (ABA) (PubMed:8075396).
Up-regulated by viral infection (PubMed:15805473).
Developmental stage
Down-regulated during seed maturation. Up-regulated during germination.
Gene expression databases
Interaction
Subunit
Binds to the deubiquitinating enzyme AMSH3. Interacts with SGT1B (via SGS domain). Interacts with OEP61. Interacts with HSFA1A/HSF1. Interacts with BAG3, BAG4 and BAG5. Interacts with WPP1, WPP2 and WIT1. Component of a ternary complex composed of WPP1, HSP70-1 and WIT1. Binds to TIR.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P22953 | SGT1B Q9SUT5 | 3 | EBI-1238845, EBI-1581364 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 618-651 | Disordered | ||||
Sequence: QGAGGEAGGPGASGMDDDAPPASGGAGPKIEEVD |
Sequence similarities
Belongs to the heat shock protein 70 (TC 1.A.33) family. DnaK subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
P22953-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length651
- Mass (Da)71,358
- Last updated2000-12-01 v3
- ChecksumBD689BA0C936A420
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4KCE5 | F4KCE5_ARATH | HSC70-1 | 521 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 65 | in Ref. 6; CAA54419 | ||||
Sequence: N → S | ||||||
Sequence conflict | 195 | in Ref. 6; CAA54419 | ||||
Sequence: S → R | ||||||
Sequence conflict | 195 | in Ref. 2; CAA52684 | ||||
Sequence: S → T | ||||||
Sequence conflict | 372 | in Ref. 6; CAA54419 | ||||
Sequence: D → V | ||||||
Sequence conflict | 376 | in Ref. 6; CAA54419 | ||||
Sequence: A → V | ||||||
Sequence conflict | 507 | in Ref. 6; CAA54419 | ||||
Sequence: I → V |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X74604 EMBL· GenBank· DDBJ | CAA52684.1 EMBL· GenBank· DDBJ | mRNA | ||
AL162971 EMBL· GenBank· DDBJ | CAB85987.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED90480.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY035123 EMBL· GenBank· DDBJ | AAK59628.2 EMBL· GenBank· DDBJ | mRNA | ||
AY057481 EMBL· GenBank· DDBJ | AAL09715.1 EMBL· GenBank· DDBJ | mRNA | ||
AY120747 EMBL· GenBank· DDBJ | AAM53305.1 EMBL· GenBank· DDBJ | mRNA | ||
BT002754 EMBL· GenBank· DDBJ | AAO22583.1 EMBL· GenBank· DDBJ | mRNA | ||
AH001335 EMBL· GenBank· DDBJ | AAA32819.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X77199 EMBL· GenBank· DDBJ | CAA54419.1 EMBL· GenBank· DDBJ | Genomic DNA |