P22724 · WNT4_MOUSE

  • Protein
    Protein Wnt-4
  • Gene
    Wnt4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed:16054034, PubMed:17537789, PubMed:19830824, PubMed:26321050, PubMed:7990960, PubMed:9989404).
Required for normal mesenchyme to epithelium transition during embryonic kidney development (PubMed:16054034, PubMed:17537789, PubMed:19830824, PubMed:7990960).
Required for the formation of early epithelial renal vesicles during kidney development (PubMed:16054034).
Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:19830824, PubMed:9989404).
Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:9989404).
Required for normal lung development and for normal patterning of trachael cartilage rings (PubMed:26321050).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell surface
Cellular Componentcytoplasm
Cellular Componentendoplasmic reticulum lumen
Cellular Componentextracellular matrix
Cellular Componentextracellular region
Cellular Componentextracellular space
Molecular Functioncytokine activity
Molecular Functionfrizzled binding
Molecular Functionsignaling receptor binding
Molecular Functiontranscription corepressor activity
Biological Processadrenal gland development
Biological Processapoptotic signaling pathway
Biological Processbranching involved in ureteric bud morphogenesis
Biological Processbranching morphogenesis of an epithelial tube
Biological Processcanonical Wnt signaling pathway
Biological Processcell differentiation
Biological Processcell fate commitment
Biological Processcell-cell signaling
Biological Processcellular response to starvation
Biological Processcellular response to transforming growth factor beta stimulus
Biological Processembryonic epithelial tube formation
Biological Processepithelial to mesenchymal transition
Biological Processfemale gonad development
Biological Processfemale sex determination
Biological Processfibroblast growth factor receptor signaling pathway
Biological Processgamete generation
Biological Processhormone metabolic process
Biological Processimmature T cell proliferation in thymus
Biological Processkidney morphogenesis
Biological Processliver development
Biological Processmale gonad development
Biological Processmeiotic nuclear division
Biological Processmesenchymal to epithelial transition
Biological Processmesonephric tubule development
Biological Processmesonephros development
Biological Processmetanephric mesenchymal cell differentiation
Biological Processmetanephric nephron development
Biological Processmetanephric nephron morphogenesis
Biological Processmetanephric tubule formation
Biological Processmetanephros development
Biological Processnegative regulation of androgen biosynthetic process
Biological Processnegative regulation of apoptotic signaling pathway
Biological Processnegative regulation of cell differentiation
Biological Processnegative regulation of cell migration
Biological Processnegative regulation of DNA-templated transcription
Biological Processnegative regulation of fibroblast growth factor receptor signaling pathway
Biological Processnegative regulation of gene expression
Biological Processnegative regulation of male gonad development
Biological Processnegative regulation of steroid biosynthetic process
Biological Processnegative regulation of testicular blood vessel morphogenesis
Biological Processnegative regulation of testosterone biosynthetic process
Biological Processnegative regulation of wound healing
Biological Processnephron development
Biological Processneuron differentiation
Biological Processnon-canonical Wnt signaling pathway
Biological Processoocyte development
Biological Processparamesonephric duct development
Biological Processpericyte cell differentiation
Biological Processpositive regulation of aldosterone biosynthetic process
Biological Processpositive regulation of bone mineralization
Biological Processpositive regulation of collagen biosynthetic process
Biological Processpositive regulation of cortisol biosynthetic process
Biological Processpositive regulation of dermatome development
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of focal adhesion assembly
Biological Processpositive regulation of GTPase activity
Biological Processpositive regulation of MAPK cascade
Biological Processpositive regulation of meiotic nuclear division
Biological Processpositive regulation of osteoblast differentiation
Biological Processpositive regulation of stress fiber assembly
Biological Processregulation of cell-cell adhesion
Biological Processrenal vesicle formation
Biological Processrenal vesicle induction
Biological ProcessSertoli cell differentiation
Biological Processsex differentiation
Biological Processsignal transduction
Biological Processsmooth muscle cell differentiation
Biological Processsomatotropin secreting cell differentiation
Biological ProcessT cell differentiation in thymus
Biological Processtertiary branching involved in mammary gland duct morphogenesis
Biological Processthyroid-stimulating hormone-secreting cell differentiation
Biological Processtube morphogenesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein Wnt-4

Gene names

    • Name
      Wnt4
    • Synonyms
      Wnt-4

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P22724

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Mutant embryos show normal early stages of kidney development, but later stages of kidney development are disrupted (PubMed:19830824, PubMed:7990960).
Mutant embryos develop to term, but the pups die within 24 hours after birth, probably due to the absence of functional kidneys (PubMed:7990960).
Reproductive organs in newborn males appear normal (PubMed:9989404).
External genitalia from newborn females appear normal, but they lack a Mullerian duct and their gonads and sex ducts appear masculinized (PubMed:19830824, PubMed:9989404).
Their ovaries contain less than 10% of the normal number of oocytes, and these are in the process of degenerating (PubMed:9989404).
Mutant embryos display altered patterning of tracheal cartilage rings. By 13.5 dpc the size of their lungs is significantly reduced, and at 17.5 dpc the left lung lobe is on average 35% smaller than for wild-type (PubMed:26321050).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 9 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, glycosylation, lipidation.

TypeIDPosition(s)Description
Signal1-22
ChainPRO_000004142223-351Protein Wnt-4
Disulfide bond78↔89
Glycosylation88N-linked (GlcNAc...) asparagine
Disulfide bond128↔136
Disulfide bond138↔155
Disulfide bond206↔220
Disulfide bond208↔215
Lipidation212O-palmitoleoyl serine; by PORCN
Disulfide bond280↔311
Disulfide bond296↔306
Glycosylation297N-linked (GlcNAc...) asparagine
Disulfide bond310↔350
Disulfide bond326↔341
Disulfide bond328↔338
Disulfide bond333↔334

Post-translational modification

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

In adults in lung and brain.

Developmental stage

Detected along the length of the mesonephros at 9.5 to 10.5 dpc. At 11.0 dpc, detected in the mesenchyme of the gonads in both sexes and in mesonephros. At 11.5 dpc, sex-specific differentiation of the gonads begins, and Wnt4 is down-regulated in male gonads, but not in female gonads. Detected in mesenchyme cells underlying the newly formed Mullerian duct in females, but not in the Wolffian duct in males (PubMed:9989404).
During kidney development, detected at 11.5 dpc in condensed mesenchymal cells on both sides of the stalk of the ureter. Detected on pretubular aggregates upon initiation of ureteric bud branching at 12.5 dpc. Detected on primitive tubular aggregates at 13.5 dpc. Detected on comma-shaped and S-shaped bodies by 14.5 dpc, and is restricted to kidney cortex by 16.5 dpc (PubMed:7990960).

Gene expression databases

Interaction

Subunit

Interacts with PORCN (PubMed:10866835).
Interacts with PKD1 (By similarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P22724Kl O350822EBI-1570945, EBI-1570828

Protein-protein interaction databases

Miscellaneous

Structure

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    351
  • Mass (Da)
    39,050
  • Last updated
    1991-08-01 v1
  • Checksum
    7E1C5C739BE939D9
MSPRSCLRSLRLLVFAVFSAAASNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDIRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCGCRFHWCCFVKCRQCQRLVEMHTCR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M89797
EMBL· GenBank· DDBJ
AAA40566.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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