P22724 · WNT4_MOUSE
- ProteinProtein Wnt-4
- GeneWnt4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids351 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed:16054034, PubMed:17537789, PubMed:19830824, PubMed:26321050, PubMed:7990960, PubMed:9989404).
Required for normal mesenchyme to epithelium transition during embryonic kidney development (PubMed:16054034, PubMed:17537789, PubMed:19830824, PubMed:7990960).
Required for the formation of early epithelial renal vesicles during kidney development (PubMed:16054034).
Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:19830824, PubMed:9989404).
Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:9989404).
Required for normal lung development and for normal patterning of trachael cartilage rings (PubMed:26321050).
Required for normal mesenchyme to epithelium transition during embryonic kidney development (PubMed:16054034, PubMed:17537789, PubMed:19830824, PubMed:7990960).
Required for the formation of early epithelial renal vesicles during kidney development (PubMed:16054034).
Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:19830824, PubMed:9989404).
Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:9989404).
Required for normal lung development and for normal patterning of trachael cartilage rings (PubMed:26321050).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein Wnt-4
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP22724
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mutant embryos show normal early stages of kidney development, but later stages of kidney development are disrupted (PubMed:19830824, PubMed:7990960).
Mutant embryos develop to term, but the pups die within 24 hours after birth, probably due to the absence of functional kidneys (PubMed:7990960).
Reproductive organs in newborn males appear normal (PubMed:9989404).
External genitalia from newborn females appear normal, but they lack a Mullerian duct and their gonads and sex ducts appear masculinized (PubMed:19830824, PubMed:9989404).
Their ovaries contain less than 10% of the normal number of oocytes, and these are in the process of degenerating (PubMed:9989404).
Mutant embryos display altered patterning of tracheal cartilage rings. By 13.5 dpc the size of their lungs is significantly reduced, and at 17.5 dpc the left lung lobe is on average 35% smaller than for wild-type (PubMed:26321050).
Mutant embryos develop to term, but the pups die within 24 hours after birth, probably due to the absence of functional kidneys (PubMed:7990960).
Reproductive organs in newborn males appear normal (PubMed:9989404).
External genitalia from newborn females appear normal, but they lack a Mullerian duct and their gonads and sex ducts appear masculinized (PubMed:19830824, PubMed:9989404).
Their ovaries contain less than 10% of the normal number of oocytes, and these are in the process of degenerating (PubMed:9989404).
Mutant embryos display altered patterning of tracheal cartilage rings. By 13.5 dpc the size of their lungs is significantly reduced, and at 17.5 dpc the left lung lobe is on average 35% smaller than for wild-type (PubMed:26321050).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 9 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-22 | |||||
Sequence: MSPRSCLRSLRLLVFAVFSAAA | ||||||
Chain | PRO_0000041422 | 23-351 | Protein Wnt-4 | |||
Sequence: SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDIRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCGCRFHWCCFVKCRQCQRLVEMHTCR | ||||||
Disulfide bond | 78↔89 | |||||
Sequence: CQYQFRNRRWNC | ||||||
Glycosylation | 88 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 128↔136 | |||||
Sequence: CSSGELEKC | ||||||
Disulfide bond | 138↔155 | |||||
Sequence: CDRTVHGVSPQGFQWSGC | ||||||
Disulfide bond | 206↔220 | |||||
Sequence: CKCHGVSGSCEVKTC | ||||||
Disulfide bond | 208↔215 | |||||
Sequence: CHGVSGSC | ||||||
Lipidation | 212 | O-palmitoleoyl serine; by PORCN | ||||
Sequence: S | ||||||
Disulfide bond | 280↔311 | |||||
Sequence: CEQDIRSGVLGTRGRTCNKTSKAIDGCELLCC | ||||||
Disulfide bond | 296↔306 | |||||
Sequence: CNKTSKAIDGC | ||||||
Glycosylation | 297 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 310↔350 | |||||
Sequence: CCGRGFHTAQVELAERCGCRFHWCCFVKCRQCQRLVEMHTC | ||||||
Disulfide bond | 326↔341 | |||||
Sequence: CGCRFHWCCFVKCRQC | ||||||
Disulfide bond | 328↔338 | |||||
Sequence: CRFHWCCFVKC | ||||||
Disulfide bond | 333↔334 | |||||
Sequence: CC |
Post-translational modification
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
In adults in lung and brain.
Developmental stage
Detected along the length of the mesonephros at 9.5 to 10.5 dpc. At 11.0 dpc, detected in the mesenchyme of the gonads in both sexes and in mesonephros. At 11.5 dpc, sex-specific differentiation of the gonads begins, and Wnt4 is down-regulated in male gonads, but not in female gonads. Detected in mesenchyme cells underlying the newly formed Mullerian duct in females, but not in the Wolffian duct in males (PubMed:9989404).
During kidney development, detected at 11.5 dpc in condensed mesenchymal cells on both sides of the stalk of the ureter. Detected on pretubular aggregates upon initiation of ureteric bud branching at 12.5 dpc. Detected on primitive tubular aggregates at 13.5 dpc. Detected on comma-shaped and S-shaped bodies by 14.5 dpc, and is restricted to kidney cortex by 16.5 dpc (PubMed:7990960).
During kidney development, detected at 11.5 dpc in condensed mesenchymal cells on both sides of the stalk of the ureter. Detected on pretubular aggregates upon initiation of ureteric bud branching at 12.5 dpc. Detected on primitive tubular aggregates at 13.5 dpc. Detected on comma-shaped and S-shaped bodies by 14.5 dpc, and is restricted to kidney cortex by 16.5 dpc (PubMed:7990960).
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length351
- Mass (Da)39,050
- Last updated1991-08-01 v1
- Checksum7E1C5C739BE939D9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M89797 EMBL· GenBank· DDBJ | AAA40566.1 EMBL· GenBank· DDBJ | mRNA |