P22704 · RPOC1_NOSS1
- ProteinDNA-directed RNA polymerase subunit gamma
- GenerpoC1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids625 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 1 Zn2+ ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 71 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 73 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 86 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 89 | Zn2+ (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 467 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 469 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 471 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit gamma
- EC number
- Short namesRNAP subunit gamma
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Cyanobacteriota > Cyanophyceae > Nostocales > Nostocaceae > Nostoc
Accessions
- Primary accessionP22704
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000067840 | 1-625 | DNA-directed RNA polymerase subunit gamma | |||
Sequence: MRPAQTNQFDYVKIGLASPERIRQWGERTLPNGQVVGEVTKPETINYRTLKPEMDGLFCERIFGPAKDWECHCGKYKRVRHRGIVCERCGVEVTESRVRRHRMGYIKLAAPVAHVWYLKGIPSYISILLDMPLRDVEQIVYFNSYVVLSPGNAETLTYKQLLSEDQWLEIEDQIYSEDSQLQGVEVGIGAEALLRLLADINLEQEAESLREEIGSAKGQKRAKLIKRLRVIDNFIATGSKPEWMVMTVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLARLQEILAPEIIVRNEKRMLQEAVDALIDNGRRGRTVVGANNRPLKSLSDIIEGKQGRFRQNLLGKRVDYSGRSVIVVGPKLKIHQCGLPREMAIELFQPFVINRLIRSGMVNNIKAAKKLISRNDPSVWDVLEEVIEGHPVMLNRAPTLHRLGIQAFEPILVEGRAIQLHPLVCPAFNADFDGDQMAVHVPLSLESQAEARLLMLASNNILSPATGRPIITPSQDMVLGAYYLTAENPGATKGAGKYFASLDDVIMAFQQEQIDLHAYVYVRFDGDVESDQPDTEPVKVTTNEDGSRTVLYKYRRVREDAQGNVISQYIYTTPGRVIYNKAIQEALAS |
Interaction
Subunit
In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length625
- Mass (Da)70,563
- Last updated1991-08-01 v1
- Checksum7DCFEEF7DD8D035A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M60831 EMBL· GenBank· DDBJ | AAA22033.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BA000019 EMBL· GenBank· DDBJ | BAB77961.1 EMBL· GenBank· DDBJ | Genomic DNA |