P22516 · CHL1_YEAST

Function

function

ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G2/M (PubMed:10931920).
May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres (PubMed:14742714, PubMed:15020404).
Has a specific role in chromosome segregation during meiosis II (PubMed:15226378).

Miscellaneous

Present with 98 molecules/cell in log phase SD medium.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site42-49ATP (UniProtKB | ChEBI)
Binding site280[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site298[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site308[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site350[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchromatin
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA binding
Molecular FunctionDNA helicase activity
Molecular Functioniron-sulfur cluster binding
Molecular Functionisomerase activity
Molecular Functionmetal ion binding
Biological ProcessDNA duplex unwinding
Biological Processestablishment of sister chromatid cohesion
Biological Processinterstrand cross-link repair
Biological Processmitotic G1 DNA damage checkpoint signaling
Biological Processmitotic sister chromatid cohesion

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent DNA helicase CHL1
  • EC number
  • Alternative names
    • Chromosome loss protein 1
    • Chromosome transmission fidelity protein 1
    • DNA 5'-3' helicase CHL1

Gene names

    • Name
      CHL1
    • Synonyms
      CTF1
    • ORF names
      YP8132.05
    • Ordered locus names
      YPL008W

Organism names

Accessions

  • Primary accession
    P22516
  • Secondary accessions
    • D6W404

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis47No ATPase activity; increase in chromosome missegregation.
Mutagenesis48No ATPase activity; increase in chromosome missegregation.

Variants

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The viewer provides 20 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000551381-861ATP-dependent DNA helicase CHL1
Modified residue86Phosphoserine
Modified residue172Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts with ECO1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P22516ECO1 P436052EBI-4600, EBI-22988

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain, region, motif.

TypeIDPosition(s)Description
Domain6-458Helicase ATP-binding
Region161-188Disordered
Motif393-396DEAH box

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    861
  • Mass (Da)
    98,806
  • Last updated
    1991-08-01 v1
  • Checksum
    15D0CC397A77F8F2
MDKKEYSETFYHPYKPYDIQVQLMETVYRVLSEGKKIAILESPTGTGKTLSLICATMTWLRMNKADIFTRMETNIKTNEDDSENLSDDEPDWVIDTYRKSVLQEKVDLLNDYEKHLNEINTTSCKQLKTMCDLDKEHGRYKSVDPLRKKRKGARHLDVSLEEQDFIPRPYESDSENNDTSKSTRGGRISDKDYKLSELNSQIITLLDKIDGKVSRDPNNGDRFDVTNQNPVKIYYASRTYSQLGQFTSQLRLPSFPSSFRDKVPDEKVKYLPLASKKQLCINPKVMKWKTLEAINDACADLRHSKEGCIFYQNTNEWRHCPDTLALRDMIFSEIQDIEDLVPLGKSLGICPYYASREALPIAEVVTLPYQYLLSESTRSSLQINLENSIVIIDEAHNLIETINSIYSSQISLEDLKNCHKGIVTYFNKFKSRLNPGNRVNLLKLNSLLMTLIQFIVKNFKKIGQEIDPNDMFTGSNIDTLNIHKLLRYIKVSKIAYKIDTYNQALKEEESSKNENPIKETHKKSVSSQPLLFKVSQFLYCLTNLTSEGQFFFEKNYSIKYMLLEPSKPFESILNQAKCVVLAGGTMEPMSEFLSNLLPEVPSEDITTLSCNHVIPKENLQTYITNQPELEFTFEKRMSPSLVNNHLFQFFVDLSKAVPKKGGIVAFFPSYQYLAHVIQCWKQNDRFATLNNVRKIFYEAKDGDDILSGYSDSVAEGRGSLLLAIVGGKLSEGINFQDDLCRAVVMVGLPFPNIFSGELIVKRKHLAAKIMKSGGTEEEASRATKEFMENICMKAVNQSVGRAIRHANDYANIYLLDVRYNRPNFRKKLSRWVQDSINSEHTTHQVISSTRKFFSMRSLNSR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U33335
EMBL· GenBank· DDBJ
AAB68097.1
EMBL· GenBank· DDBJ
Genomic DNA
X56584
EMBL· GenBank· DDBJ
CAA39922.1
EMBL· GenBank· DDBJ
Genomic DNA
Z48483
EMBL· GenBank· DDBJ
CAA88378.1
EMBL· GenBank· DDBJ
Genomic DNA
Z71255
EMBL· GenBank· DDBJ
CAA95033.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006949
EMBL· GenBank· DDBJ
DAA11420.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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