P22516 · CHL1_YEAST
- ProteinATP-dependent DNA helicase CHL1
- GeneCHL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids861 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G2/M (PubMed:10931920).
May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres (PubMed:14742714, PubMed:15020404).
Has a specific role in chromosome segregation during meiosis II (PubMed:15226378).
May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres (PubMed:14742714, PubMed:15020404).
Has a specific role in chromosome segregation during meiosis II (PubMed:15226378).
Miscellaneous
Present with 98 molecules/cell in log phase SD medium.
Catalytic activity
- Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Note: Binds 1 [4Fe-4S] cluster.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 42-49 | ATP (UniProtKB | ChEBI) | ||||
Sequence: SPTGTGKT | ||||||
Binding site | 280 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 298 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 308 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 350 | [4Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromatin | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Molecular Function | iron-sulfur cluster binding | |
Molecular Function | isomerase activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA duplex unwinding | |
Biological Process | establishment of sister chromatid cohesion | |
Biological Process | interstrand cross-link repair | |
Biological Process | mitotic G1 DNA damage checkpoint signaling | |
Biological Process | mitotic sister chromatid cohesion |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent DNA helicase CHL1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP22516
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 47 | No ATPase activity; increase in chromosome missegregation. | ||||
Sequence: G → A or V | ||||||
Mutagenesis | 48 | No ATPase activity; increase in chromosome missegregation. | ||||
Sequence: K → R |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 20 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000055138 | 1-861 | ATP-dependent DNA helicase CHL1 | |||
Sequence: MDKKEYSETFYHPYKPYDIQVQLMETVYRVLSEGKKIAILESPTGTGKTLSLICATMTWLRMNKADIFTRMETNIKTNEDDSENLSDDEPDWVIDTYRKSVLQEKVDLLNDYEKHLNEINTTSCKQLKTMCDLDKEHGRYKSVDPLRKKRKGARHLDVSLEEQDFIPRPYESDSENNDTSKSTRGGRISDKDYKLSELNSQIITLLDKIDGKVSRDPNNGDRFDVTNQNPVKIYYASRTYSQLGQFTSQLRLPSFPSSFRDKVPDEKVKYLPLASKKQLCINPKVMKWKTLEAINDACADLRHSKEGCIFYQNTNEWRHCPDTLALRDMIFSEIQDIEDLVPLGKSLGICPYYASREALPIAEVVTLPYQYLLSESTRSSLQINLENSIVIIDEAHNLIETINSIYSSQISLEDLKNCHKGIVTYFNKFKSRLNPGNRVNLLKLNSLLMTLIQFIVKNFKKIGQEIDPNDMFTGSNIDTLNIHKLLRYIKVSKIAYKIDTYNQALKEEESSKNENPIKETHKKSVSSQPLLFKVSQFLYCLTNLTSEGQFFFEKNYSIKYMLLEPSKPFESILNQAKCVVLAGGTMEPMSEFLSNLLPEVPSEDITTLSCNHVIPKENLQTYITNQPELEFTFEKRMSPSLVNNHLFQFFVDLSKAVPKKGGIVAFFPSYQYLAHVIQCWKQNDRFATLNNVRKIFYEAKDGDDILSGYSDSVAEGRGSLLLAIVGGKLSEGINFQDDLCRAVVMVGLPFPNIFSGELIVKRKHLAAKIMKSGGTEEEASRATKEFMENICMKAVNQSVGRAIRHANDYANIYLLDVRYNRPNFRKKLSRWVQDSINSEHTTHQVISSTRKFFSMRSLNSR | ||||||
Modified residue | 86 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 172 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with ECO1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P22516 | ECO1 P43605 | 2 | EBI-4600, EBI-22988 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-458 | Helicase ATP-binding | ||||
Sequence: YSETFYHPYKPYDIQVQLMETVYRVLSEGKKIAILESPTGTGKTLSLICATMTWLRMNKADIFTRMETNIKTNEDDSENLSDDEPDWVIDTYRKSVLQEKVDLLNDYEKHLNEINTTSCKQLKTMCDLDKEHGRYKSVDPLRKKRKGARHLDVSLEEQDFIPRPYESDSENNDTSKSTRGGRISDKDYKLSELNSQIITLLDKIDGKVSRDPNNGDRFDVTNQNPVKIYYASRTYSQLGQFTSQLRLPSFPSSFRDKVPDEKVKYLPLASKKQLCINPKVMKWKTLEAINDACADLRHSKEGCIFYQNTNEWRHCPDTLALRDMIFSEIQDIEDLVPLGKSLGICPYYASREALPIAEVVTLPYQYLLSESTRSSLQINLENSIVIIDEAHNLIETINSIYSSQISLEDLKNCHKGIVTYFNKFKSRLNPGNRVNLLKLNSLLMTLIQFIVKN | ||||||
Region | 161-188 | Disordered | ||||
Sequence: EEQDFIPRPYESDSENNDTSKSTRGGRI | ||||||
Motif | 393-396 | DEAH box | ||||
Sequence: DEAH |
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length861
- Mass (Da)98,806
- Last updated1991-08-01 v1
- Checksum15D0CC397A77F8F2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U33335 EMBL· GenBank· DDBJ | AAB68097.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X56584 EMBL· GenBank· DDBJ | CAA39922.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z48483 EMBL· GenBank· DDBJ | CAA88378.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z71255 EMBL· GenBank· DDBJ | CAA95033.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006949 EMBL· GenBank· DDBJ | DAA11420.1 EMBL· GenBank· DDBJ | Genomic DNA |