P22146 · GAS1_YEAST

Function

function

Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall. Involved in cell wall biosynthesis and morphogenesis.

Miscellaneous

Present with 11000 wall-bound molecules/cell in log phase YPD medium.

Features

Showing features for binding site, active site.

155950100150200250300350400450500550
TypeIDPosition(s)Description
Binding site92(1,3-beta-D-glucosyl)n 1 (UniProtKB | ChEBI); donor substrate
Binding site119-127(1,3-beta-D-glucosyl)n 1 (UniProtKB | ChEBI); donor substrate
Binding site160(1,3-beta-D-glucosyl)n 1 (UniProtKB | ChEBI); donor substrate
Active site161Proton donor
Binding site161(1,3-beta-D-glucosyl)n 2 (UniProtKB | ChEBI); acceptor substrate
Binding site202(1,3-beta-D-glucosyl)n 2 (UniProtKB | ChEBI); acceptor substrate
Binding site207(1,3-beta-D-glucosyl)n 2 (UniProtKB | ChEBI); acceptor substrate
Active site262Nucleophile
Binding site294(1,3-beta-D-glucosyl)n 1 (UniProtKB | ChEBI); donor substrate

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcellular bud scar
Cellular ComponentCOPII-coated ER to Golgi transport vesicle
Cellular Componentextracellular region
Cellular Componentfungal-type cell wall
Cellular Componentmembrane raft
Cellular Componentmitochondrion
Cellular Componentnuclear periphery
Cellular Componentplasma membrane
Cellular Componentprimary cell septum
Cellular Componentside of membrane
Molecular Function1,3-beta-glucanosyltransferase activity
Biological Processfilamentous growth
Biological Processfungal-type cell wall (1->3)-beta-D-glucan biosynthetic process
Biological Processfungal-type cell wall organization
Biological Processheterochromatin formation
Biological Processregulation of response to endoplasmic reticulum stress

Keywords

Enzyme and pathway databases

Protein family/group databases

    • CBM43Carbohydrate-Binding Module Family 43
    • GH72Glycoside Hydrolase Family 72

Names & Taxonomy

Protein names

  • Recommended name
    1,3-beta-glucanosyltransferase GAS1
  • EC number
  • Alternative names
    • Glycolipid-anchored surface protein 1
    • Glycoprotein GP115

Gene names

    • Name
      GAS1
    • Synonyms
      GGP1
    • ORF names
      YM9952.09
    • Ordered locus names
      YMR307W

Organism names

Accessions

  • Primary accession
    P22146
  • Secondary accessions
    • D6W0D4
    • P23151

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Lipid-anchor, GPI-anchor
Secreted, cell wall
Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as covalently-linked GPI-modified cell wall protein (GPI-CWP) in the outer cell wall layer.

Keywords

Phenotypes & Variants

Disruption phenotype

Increases cellular chitin level (PubMed:17142567).
Increases chitin chain length (PubMed:17142567).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis74Impairs the folding and stability of the protein.
Mutagenesis103Partially impairs the folding and stability of the protein.
Mutagenesis161Loss of function.
Mutagenesis262Loss of function.
Mutagenesis265Partially impairs the folding and stability of the protein.
Mutagenesis348Impairs the folding and stability of the protein.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 4 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for signal, chain, glycosylation, disulfide bond, lipidation, propeptide.

TypeIDPosition(s)Description
Signal1-22
ChainPRO_000001047323-5281,3-beta-glucanosyltransferase GAS1
Glycosylation40N-linked (GlcNAc...) asparagine
Glycosylation57N-linked (GlcNAc...) asparagine
Disulfide bond74↔103
Glycosylation95N-linked (GlcNAc...) asparagine
Glycosylation149N-linked (GlcNAc...) asparagine
Glycosylation165N-linked (GlcNAc...) asparagine
Disulfide bond216↔348
Disulfide bond234↔265
Glycosylation253N-linked (GlcNAc...) asparagine
Glycosylation283N-linked (GlcNAc...) asparagine
Glycosylation321N-linked (GlcNAc...) asparagine
Disulfide bond370↔421
Disulfide bond372↔462
Disulfide bond379↔445
Disulfide bond398↔403
Glycosylation409N-linked (GlcNAc...) asparagine
Glycosylation495N-linked (GlcNAc...) asparagine
Lipidation528GPI-anchor amidated asparagine
PropeptidePRO_0000010474529-559Removed in mature form

Post-translational modification

Extensively N- and O-glycosylated.
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords

Proteomic databases

PTM databases

Interaction

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P22146SIR2 P067002EBI-7327, EBI-17219

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region474-526Disordered

Sequence similarities

Belongs to the glycosyl hydrolase 72 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    559
  • Mass (Da)
    59,582
  • Last updated
    1996-02-01 v2
  • Checksum
    D6E39568DCB4C5AE
MLFKSLSKLATAAAFFAGVATADDVPAIEVVGNKFFYSNNGSQFYIRGVAYQADTANETSGSTVNDPLANYESCSRDIPYLKKLNTNVIRVYAINTTLDHSECMKALNDADIYVIADLAAPATSINRDDPTWTVDLFNSYKTVVDTFANYTNVLGFFAGNEVTNNYTNTDASAFVKAAIRDVRQYISDKNYRKIPVGYSSNDDEDTRVKMTDYFACGDDDVKADFYGINMYEWCGKSDFKTSGYADRTAEFKNLSIPVFFSEYGCNEVTPRLFTEVEALYGSNMTDVWSGGIVYMYFEETNKYGLVSIDGNDVKTLDDFNNYSSEINKISPTSANTKSYSATTSDVACPATGKYWSAATELPPTPNGGLCSCMNAANSCVVSDDVDSDDYETLFNWICNEVDCSGISANGTAGKYGAYSFCTPKEQLSFVMNLYYEKSGGSKSDCSFSGSATLQTATTQASCSSALKEIGSMGTNSASGSVDLGSGTESSTASSNASGSSSKSNSGSSGSSSSSSSSSASSSSSSKKNAATNVKANLAQVVFTSIISLSIAAGVGFALV

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict211in Ref. 1; CAA37512

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X53424
EMBL· GenBank· DDBJ
CAA37512.1
EMBL· GenBank· DDBJ
Genomic DNA
X56399
EMBL· GenBank· DDBJ
CAA39809.1
EMBL· GenBank· DDBJ
Genomic DNA
Z49212
EMBL· GenBank· DDBJ
CAA89140.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006946
EMBL· GenBank· DDBJ
DAA10208.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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