P21965 · MCK1_YEAST
- ProteinProtein kinase MCK1
- GeneMCK1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids375 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
May be an autophosphorylating tyrosine kinase, a bifunctional (serine/tyrosine-specific) protein kinase, or a serine kinase that is a substrate for an associated tyrosine kinase. MCK1 is a transcriptional activator of IME1, it stimulates spore maturation, and play a positive regulatory role in both mitotic centromere function and activation of early meiotic gene expression.
Miscellaneous
Present with 396 molecules/cell in log phase SD medium.
Catalytic activity
- L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | cyclin binding | |
Molecular Function | enzyme inhibitor activity | |
Molecular Function | protein kinase activity | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Molecular Function | protein serine/threonine/tyrosine kinase activity | |
Molecular Function | protein tyrosine kinase activity | |
Biological Process | ascospore formation | |
Biological Process | cell differentiation | |
Biological Process | double-strand break repair via nonhomologous end joining | |
Biological Process | meiotic cell cycle | |
Biological Process | mitotic sister chromatid segregation | |
Biological Process | mitotic spindle orientation checkpoint signaling | |
Biological Process | protein catabolic process | |
Biological Process | protein phosphorylation | |
Biological Process | signal transduction |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein kinase MCK1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP21965
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000086317 | 2-375 | Protein kinase MCK1 | |||
Sequence: STEEQNGVPLQRGSEFIADDVTSNKSNNTRRMLVKEYRKIGRGAFGTVVQAYLTQDKKNWLGPFAIKKVPAHTEYKSRELQILRIADHPNIVKLQYFFTHLSPQDNKVYQHLAMECLPETLQIEINRYVTNKLEMPLKHIRLYTYQIARGMLYLHGLGVCHRDIKPSNVLVDPETGVLKICDFGSAKKLEHNQPSISYICSRFYRAPELIIGCTQYTTQIDIWGLGCVMGEMLIGKAIFQGQEPLLQLREIAKLLGPPDKRFIFFSNPAYDGPLFSKPLFSGSSQQRFEKYFGHSGPDGIDLLMKILVYEPQQRLSPRRILAHQFFNELRNDDTFLPRGFTEPIKLPNLFDFNDFELQILGEFADKIKPTKVAE | ||||||
Modified residue | 198 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 199 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 202 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated at tyrosine and serine.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P21965 | CDC14 Q00684 | 2 | EBI-10517, EBI-4192 | |
BINARY | P21965 | UBR1 P19812 | 3 | EBI-10517, EBI-19909 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 35-327 | Protein kinase | ||||
Sequence: VKEYRKIGRGAFGTVVQAYLTQDKKNWLGPFAIKKVPAHTEYKSRELQILRIADHPNIVKLQYFFTHLSPQDNKVYQHLAMECLPETLQIEINRYVTNKLEMPLKHIRLYTYQIARGMLYLHGLGVCHRDIKPSNVLVDPETGVLKICDFGSAKKLEHNQPSISYICSRFYRAPELIIGCTQYTTQIDIWGLGCVMGEMLIGKAIFQGQEPLLQLREIAKLLGPPDKRFIFFSNPAYDGPLFSKPLFSGSSQQRFEKYFGHSGPDGIDLLMKILVYEPQQRLSPRRILAHQFF |
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length375
- Mass (Da)43,136
- Last updated1991-08-01 v1
- ChecksumBCF0C776B6E3841B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X55054 EMBL· GenBank· DDBJ | CAA38895.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M55984 EMBL· GenBank· DDBJ | AAA34764.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z46259 EMBL· GenBank· DDBJ | CAA86388.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z71583 EMBL· GenBank· DDBJ | CAA96236.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z71582 EMBL· GenBank· DDBJ | CAA96235.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006947 EMBL· GenBank· DDBJ | DAA10254.1 EMBL· GenBank· DDBJ | Genomic DNA |