P21947 · REP_ABMVW
- ProteinReplication-associated protein
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids356 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the + strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity).
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Divalent metal cations, possibly Mg2+ or Mn2+.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 49 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 57 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 59 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 103 | For DNA cleavage activity | ||||
Sequence: Y | ||||||
Binding site | 107 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 221-228 | ATP (UniProtKB | ChEBI) | ||||
Sequence: EGDSRTGK |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | endodeoxyribonuclease activity, producing 5'-phosphomonoesters | |
Molecular Function | helicase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotidyltransferase activity | |
Molecular Function | structural molecule activity | |
Biological Process | DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplication-associated protein
- EC number
- Short namesRep
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Monodnaviria > Shotokuvirae > Cressdnaviricota > Repensiviricetes > Geplafuvirales > Geminiviridae > Begomovirus > Begomovirus bauri
- Virus hosts
Accessions
- Primary accessionP21947
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000222200 | 1-356 | Replication-associated protein | |||
Sequence: MPPPKKFRVQAKNYFLTYPQCSLTKDEALSQLQNLETPVNKKFIKICRELHENGEPHLHVLIQFEGKYQCTNNRFFDLVSPTRSAHFHPNIQGAKSSSDVKSYIDKDGDTAEWGEFQIDGRSARGGQQTANDSYAKALNAGDVQSALNILKEEQPKDYVLQNHNIRSNLERIFAKAPEPWVPRFPLSSFTAVPEEMQEWADDYFGSGSAARPDRPLSLIVEGDSRTGKTMWARALGPHNYLSGHLDFNGRVYSNEVEYNVIDDVAPHYLKLKHWKELLGAQKDWQSNCKYGKPVQIKGGIPAIVLCNPGEGSSYKEYLDKEENTGLRNWTLKNAIFITLTAPLYQEGTQAGQEEGH |
Keywords
- PTM
Interaction
Subunit
Homooligomer. Interacts with the replication enhancer protein (REn). Interacts with host retinoblastoma-related protein 1 (RBR1), and may thereby induce the transcription of host replicative enzymes even if the cell is not dividing anymore. Interacts with host PCNA. Interacts with host SCE1 protein (By similarity).
Family & Domains
Features
Showing features for domain, motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 8-116 | CRESS-DNA virus Rep endonuclease | ||||
Sequence: RVQAKNYFLTYPQCSLTKDEALSQLQNLETPVNKKFIKICRELHENGEPHLHVLIQFEGKYQCTNNRFFDLVSPTRSAHFHPNIQGAKSSSDVKSYIDKDGDTAEWGEF | ||||||
Motif | 15-18 | RCR-1 | ||||
Sequence: FLTY | ||||||
Motif | 57-59 | RCR-2 | ||||
Sequence: HLH | ||||||
Motif | 103-106 | RCR-3 | ||||
Sequence: YIDK | ||||||
Region | 143-153 | Binding to RBR1 | ||||
Sequence: VQSALNILKEE | ||||||
Region | 156-176 | Oligomerization | ||||
Sequence: KDYVLQNHNIRSNLERIFAKA |
Domain
There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR (By similarity).
Sequence similarities
Belongs to the geminiviridae Rep protein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length356
- Mass (Da)40,247
- Last updated2008-02-26 v2
- Checksum3AA732F65867352A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X15983 EMBL· GenBank· DDBJ | CAA34111.2 EMBL· GenBank· DDBJ | Genomic DNA |