P21698 · NSS_RVFVZ
- ProteinNon-structural protein S
- GeneNSS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids265 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a role in the escape of host innate immune response by promoting the degradation of host EIF2AK2/PKR and inhibiting host transcription (PubMed:19197350, PubMed:19211744, PubMed:19751406).
Cytoplasmic NSs interacts with host FBXW11 to degrade PKR whereas nuclear pool binds to host FBXO3 to target TFIIH subunit GTF2H1 for proteasomal degradation with the help of the linker protein SKP1 (PubMed:19211744, PubMed:21543505, PubMed:23063407, PubMed:26837067).
Removes FBXO3 isoform 1 from the nucleus (PubMed:24403578).
Forms nuclear amyloid-like filaments of about 12 nm in width that may sequester NSs binding partners, causing cell cycle arrest (PubMed:10233964, PubMed:28915104, PubMed:32612175).
Also aggragates in the cytosol as short fibrils late after host cell infection (PubMed:32612175).
Plays a role in cell morphology and/or motility, reduction of lamellipodia, cell spreading, and dissolution of adherens junctions (PubMed:33087469).
Cytoplasmic NSs interacts with host FBXW11 to degrade PKR whereas nuclear pool binds to host FBXO3 to target TFIIH subunit GTF2H1 for proteasomal degradation with the help of the linker protein SKP1 (PubMed:19211744, PubMed:21543505, PubMed:23063407, PubMed:26837067).
Removes FBXO3 isoform 1 from the nucleus (PubMed:24403578).
Forms nuclear amyloid-like filaments of about 12 nm in width that may sequester NSs binding partners, causing cell cycle arrest (PubMed:10233964, PubMed:28915104, PubMed:32612175).
Also aggragates in the cytosol as short fibrils late after host cell infection (PubMed:32612175).
Plays a role in cell morphology and/or motility, reduction of lamellipodia, cell spreading, and dissolution of adherens junctions (PubMed:33087469).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | host cell cytoplasm | |
Cellular Component | host cell nucleus | |
Molecular Function | protein serine/threonine kinase inhibitor activity | |
Biological Process | negative stranded viral RNA replication | |
Biological Process | suppression by virus of host type I interferon production | |
Biological Process | symbiont-mediated suppression of host gene expression | |
Biological Process | symbiont-mediated suppression of host PKR/eIFalpha signaling | |
Biological Process | symbiont-mediated suppression of host transcription | |
Biological Process | symbiont-mediated suppression of host transcription initiation from RNA polymerase II promoter | |
Biological Process | symbiont-mediated suppression of host type I interferon-mediated signaling pathway |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameNon-structural protein S
- Short namesNSs
Gene names
Organism names
- Strains
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Ellioviricetes > Bunyavirales > Phenuiviridae > Phlebovirus > Phlebovirus riftense
- Virus hosts
Accessions
- Primary accessionP21698
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 173 | Complete loss of binding to host EIF2AK2/PKR. | ||||
Sequence: R → A | ||||||
Mutagenesis | 261 | Loss of formation of nuclear filaments and GTF2H1 degradation. Decreased virulence. | ||||
Sequence: F → P or S |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000221979 | 1-265 | Non-structural protein S | |||
Sequence: MDYFPVISVDLQSGRRVVSVEYFRGDGPPRIPYSMVGPCCVFLMHHRPSHEVRLRFSDFYNVGEFPYRVGLGDFASNVAPPPAKPFQRLIDLIGHMTLSDFTRFPNLKEAISWPLGEPSLAFFDLSSTRVHRNDDIRRDQIATLAMRSCKITNDLEDSFVGLHRMIATEAILRGIDLCLLPGFDLMYEVAHVQCVRLLQAAKEDISNAVVPNSALIVLMEESLMLRSSLPSMMGRNNWIPVIPPIPDVEMESEEESDDDGFVEVD | ||||||
Modified residue | 252 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 256 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated.
Keywords
- PTM
PTM databases
Interaction
Subunit
Multimerizes; forms 0.5-1 mm thick proteinaceous filaments in the nucleus (PubMed:10233964, PubMed:28915104).
Interacts (via omegaXaV motif) with host FBXW11; this interaction is important for EIF2AK2/PKR degradation (PubMed:21543505, PubMed:26837067).
Interacts (via omegaXaV motif) with host GTF2H1 (PubMed:25918396).
Interacts with the host E3 ubiquitin ligase component FBXO3; this interaction is important for GTF2H1 degradation (PubMed:24403578).
Interacts (via omegaXaV motif) with host FBXW11; this interaction is important for EIF2AK2/PKR degradation (PubMed:21543505, PubMed:26837067).
Interacts (via omegaXaV motif) with host GTF2H1 (PubMed:25918396).
Interacts with the host E3 ubiquitin ligase component FBXO3; this interaction is important for GTF2H1 degradation (PubMed:24403578).
Protein-protein interaction databases
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 249-265 | Disordered | ||||
Sequence: EMESEEESDDDGFVEVD | ||||||
Motif | 261-265 | OmegaXaV | ||||
Sequence: FVEVD |
Domain
The C-terminus disordered region is involved in multimerization, filament formation, but not nuclear localization (PubMed:10233964).
OmegaXaV motif is required for both nuclear filament formation and degradation of host GTF2H1 (PubMed:25918396).
This motif also mediates FBXW11 binding (PubMed:26837067).
OmegaXaV motif is required for both nuclear filament formation and degradation of host GTF2H1 (PubMed:25918396).
This motif also mediates FBXW11 binding (PubMed:26837067).
Sequence similarities
Belongs to the phlebovirus NS-S protein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length265
- Mass (Da)29,931
- Last updated2019-06-05 v2
- ChecksumCD65B9FA3E614435
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 160 | in Ref. 1; CAA37788 | ||||
Sequence: V → A |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X53771 EMBL· GenBank· DDBJ | CAA37788.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
DQ380143 EMBL· GenBank· DDBJ | ABD38716.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
KY366328 EMBL· GenBank· DDBJ | ASF20659.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
EU312138 EMBL· GenBank· DDBJ | ACA14424.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
EU312137 EMBL· GenBank· DDBJ | ACA14422.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
EU312134 EMBL· GenBank· DDBJ | ACA14416.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
EU312112 EMBL· GenBank· DDBJ | ACA14372.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
EU312110 EMBL· GenBank· DDBJ | ACA14368.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
DQ380149 EMBL· GenBank· DDBJ | ABD38728.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
DQ380148 EMBL· GenBank· DDBJ | ABD38726.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
DQ380147 EMBL· GenBank· DDBJ | ABD38724.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
DQ380144 EMBL· GenBank· DDBJ | ABD38718.1 EMBL· GenBank· DDBJ | Genomic RNA |