P21269 · CCA1_YEAST
- ProteinCCA tRNA nucleotidyltransferase, mitochondrial
- GeneCCA1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids546 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates (PubMed:1634528, PubMed:23872483).
tRNA 3'-terminal CCA addition is required both for tRNA processing and repair (By similarity).
Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs (By similarity).
While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded (By similarity).
The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA (By similarity).
This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle (By similarity).
tRNA 3'-terminal CCA addition is required both for tRNA processing and repair (By similarity).
Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs (By similarity).
While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded (By similarity).
The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA (By similarity).
This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle (By similarity).
Miscellaneous
Present with 13500 molecules/cell in log phase SD medium.
Catalytic activity
- a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial matrix | |
Cellular Component | mitochondrion | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity | |
Molecular Function | CCA tRNA nucleotidyltransferase activity | |
Molecular Function | CTP:tRNA cytidylyltransferase activity | |
Molecular Function | RNA binding | |
Biological Process | tRNA 3'-terminal CCA addition |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCCA tRNA nucleotidyltransferase, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP21269
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Isoform Mitochondrial
Isoform Cytoplasmic+nuclear 1
Isoform Cytoplasmic+nuclear 2
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 64 | No effect. Compensates K-189 mutant; when associated with K-189. | ||||
Sequence: R → N | ||||||
Mutagenesis | 189 | Temperature-sensitive mutation; reduced ability to incorporate AMP and CMP at 37 degrees Celsius. Defects are compensated by mutant N-64; when associated with N-64. | ||||
Sequence: E → K |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 4 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, transit peptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000004779 | ?-546 | CCA tRNA nucleotidyltransferase, mitochondrial | |||
Sequence: MLRSTISLLMNSAAQKTMTNSNFVLNAPKITLTKVEQNICNLLNDYTDLYNQKYHNKPEPLTLRITGGWVRDKLLGQGSHDLDIAINVMSGEQFATGLNEYLQQHYAKYGAKPHNIHKIDKNPEKSKHLETATTKLFGVEVDFVNLRSEKYTELSRIPKVCFGTPEEDALRRDATLNALFYNIHKGEVEDFTKRGLQDLKDGVLRTPLPAKQTFLDDPLRVLRLIRFASRFNFTIDPEVMAEMGDPQINVAFNSKISRERVGVEMEKILVGPTPLLALQLIQRAHLENVIFFWHNDSSVVKFNEENCQDMDKINHVYNDNILNSHLKSFIELYPMFLEKLPILREKIGRSPGFQQNFILSAILSPMANLQIIGNPKKKINNLVSVTESIVKEGLKLSKNDAAVIAKTVDSICSYEEILAKFADRSQLKKSEIGIFLRNFNGEWETAHFASLSDAFLKIPKLETKKIELLFQNYNEFYSYIFDNNLNNCHELKPIVDGKQMAKLLQMKPGPWLGKINNEAIRWQFDNPTGTDQELITHLKAILPKYL | ||||||
Transit peptide | 1-? | Mitochondrion |
Proteomic databases
PTM databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P21269 | ENO2 P00925 | 2 | EBI-4347, EBI-6475 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 3 isoforms produced by Alternative initiation.
P21269-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameMitochondrial
- Length546
- Mass (Da)62,485
- Last updated1991-05-01 v1
- ChecksumC0B2B918596E19D5
P21269-2
- NameCytoplasmic+nuclear 1
- Differences from canonical
- 1-9: Missing
P21269-3
- NameCytoplasmic+nuclear 2
- Differences from canonical
- 1-17: Missing
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_018698 | 1-9 | in isoform Cytoplasmic+nuclear 1 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_018699 | 1-17 | in isoform Cytoplasmic+nuclear 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M59870 EMBL· GenBank· DDBJ | AAA35160.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U18922 EMBL· GenBank· DDBJ | AAB64695.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006939 EMBL· GenBank· DDBJ | DAA07830.1 EMBL· GenBank· DDBJ | Genomic DNA |