P21269 · CCA1_YEAST

Function

function

Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates (PubMed:1634528, PubMed:23872483).
tRNA 3'-terminal CCA addition is required both for tRNA processing and repair (By similarity).
Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs (By similarity).
While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded (By similarity).
The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA (By similarity).
This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle (By similarity).

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.

GO annotations

AspectTerm
Cellular Componentmitochondrial matrix
Cellular Componentmitochondrion
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctionCTP:tRNA cytidylyltransferase activity
Molecular FunctionRNA binding
Biological ProcesstRNA 3'-terminal CCA addition

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CCA tRNA nucleotidyltransferase, mitochondrial
  • EC number
  • Alternative names
    • CCA-adding enzyme
    • tRNA CCA-pyrophosphorylase
    • tRNA adenylyltransferase
    • tRNA nucleotidyltransferase

Gene names

    • Name
      CCA1
    • Synonyms
      TNT1
    • Ordered locus names
      YER168C

Organism names

Accessions

  • Primary accession
    P21269
  • Secondary accessions
    • D3DM76

Proteomes

Organism-specific databases

Subcellular Location

Isoform Mitochondrial

Mitochondrion

Isoform Cytoplasmic+nuclear 1

Cytoplasm
Nucleus

Isoform Cytoplasmic+nuclear 2

Cytoplasm
Nucleus

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis64No effect. Compensates K-189 mutant; when associated with K-189.
Mutagenesis189Temperature-sensitive mutation; reduced ability to incorporate AMP and CMP at 37 degrees Celsius. Defects are compensated by mutant N-64; when associated with N-64.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 4 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, transit peptide.

TypeIDPosition(s)Description
ChainPRO_0000004779?-546CCA tRNA nucleotidyltransferase, mitochondrial
Transit peptide1-?Mitochondrion

Proteomic databases

PTM databases

Interaction

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P21269ENO2 P009252EBI-4347, EBI-6475

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 3 isoforms produced by Alternative initiation.

P21269-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    Mitochondrial
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    546
  • Mass (Da)
    62,485
  • Last updated
    1991-05-01 v1
  • Checksum
    C0B2B918596E19D5
MLRSTISLLMNSAAQKTMTNSNFVLNAPKITLTKVEQNICNLLNDYTDLYNQKYHNKPEPLTLRITGGWVRDKLLGQGSHDLDIAINVMSGEQFATGLNEYLQQHYAKYGAKPHNIHKIDKNPEKSKHLETATTKLFGVEVDFVNLRSEKYTELSRIPKVCFGTPEEDALRRDATLNALFYNIHKGEVEDFTKRGLQDLKDGVLRTPLPAKQTFLDDPLRVLRLIRFASRFNFTIDPEVMAEMGDPQINVAFNSKISRERVGVEMEKILVGPTPLLALQLIQRAHLENVIFFWHNDSSVVKFNEENCQDMDKINHVYNDNILNSHLKSFIELYPMFLEKLPILREKIGRSPGFQQNFILSAILSPMANLQIIGNPKKKINNLVSVTESIVKEGLKLSKNDAAVIAKTVDSICSYEEILAKFADRSQLKKSEIGIFLRNFNGEWETAHFASLSDAFLKIPKLETKKIELLFQNYNEFYSYIFDNNLNNCHELKPIVDGKQMAKLLQMKPGPWLGKINNEAIRWQFDNPTGTDQELITHLKAILPKYL

P21269-2

  • Name
    Cytoplasmic+nuclear 1
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

P21269-3

  • Name
    Cytoplasmic+nuclear 2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0186981-9in isoform Cytoplasmic+nuclear 1
Alternative sequenceVSP_0186991-17in isoform Cytoplasmic+nuclear 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M59870
EMBL· GenBank· DDBJ
AAA35160.1
EMBL· GenBank· DDBJ
Genomic DNA
U18922
EMBL· GenBank· DDBJ
AAB64695.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006939
EMBL· GenBank· DDBJ
DAA07830.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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