P20840 · SAG1_YEAST
- ProteinAlpha-agglutinin
- GeneSAG1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids650 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cell surface glycoprotein promoting cell-cell contact to facilitate mating. S.cerevisiae A and alpha cells express the complementary cell surface glycoproteins A-agglutinin and alpha-, respectively, which interact with one another to promote cellular aggregation during mating.
Features
Showing features for site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Site | 348 | Not glycosylated | |||
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cell periphery | |
Cellular Component | extracellular region | |
Cellular Component | fungal-type cell wall | |
Cellular Component | fungal-type vacuole | |
Cellular Component | side of membrane | |
Molecular Function | cell adhesion molecule binding | |
Biological Process | agglutination involved in conjugation with cellular fusion |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlpha-agglutinin
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP20840
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Lipid-anchor, GPI-anchor
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 211 | Little effect on secreted alpha-agglutinin activity. | |||
Mutagenesis | 214 | No effect on secreted alpha-agglutinin activity. | |||
Mutagenesis | 215 | Little or no effect on secreted alpha-agglutinin activity. | |||
Mutagenesis | 216 | Complete loss of alpha-agglutinin activity. | |||
Mutagenesis | 216 | No effect on secreted alpha-agglutinin activity. | |||
Mutagenesis | 216 | Decreases secreted alpha-agglutinin activity by 100-fold. | |||
Mutagenesis | 216 | Decreases secreted alpha-agglutinin activity by 10-fold. | |||
Mutagenesis | 217 | Little effect on secreted alpha-agglutinin activity. | |||
Mutagenesis | 217 | Decreases secreted alpha-agglutinin activity by 10-fold. | |||
Mutagenesis | 289 | Decreases secreted alpha-agglutinin activity by less than 2-fold. | |||
Mutagenesis | 290 | Decreases secreted alpha-agglutinin activity by less than 2-fold. | |||
Mutagenesis | 291 | Decreases secreted alpha-agglutinin activity by 4-fold. | |||
Mutagenesis | 292 | Almost complete loss of secreted alpha-agglutinin activity. | |||
Mutagenesis | 294 | Decreases secreted alpha-agglutinin activity by more than 20-fold. | |||
Mutagenesis | 295 | Decreases secreted alpha-agglutinin activity by 2-fold. | |||
Mutagenesis | 296 | Decreases secreted alpha-agglutinin activity by more than 20-fold. | |||
Mutagenesis | 298 | Decreases secreted alpha-agglutinin activity by less than 2-fold. | |||
Mutagenesis | 322 | Decreases secreted alpha-agglutinin activity by 60-fold. | |||
Mutagenesis | 322 | Almost complete loss of secreted alpha-agglutinin activity by 10-fold. | |||
Mutagenesis | 322 | Decreases secreted alpha-agglutinin activity by 15-fold. | |||
Mutagenesis | 323 | Decreases secreted alpha-agglutinin activity by 2-fold. | |||
Mutagenesis | 324 | Little effect on secreted alpha-agglutinin activity. | |||
Mutagenesis | 325 | Little effect on secreted alpha-agglutinin activity. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 18 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond, lipidation, propeptide.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Signal | 1-19 | ||||
Chain | PRO_0000022265 | 20-627 | Alpha-agglutinin | ||
Glycosylation | 79 | N-linked (GlcNAc...) asparagine | |||
Disulfide bond | 97↔114 | ||||
Glycosylation | 109 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 135 | N-linked (GlcNAc...) asparagine | |||
Disulfide bond | 202↔300 | ||||
Glycosylation | 248 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 282 | O-linked (Man...) serine | |||
Glycosylation | 289 | O-linked (Man...) threonine | |||
Glycosylation | 299 | O-linked (Man...) threonine | |||
Glycosylation | 303 | O-linked (Man...) threonine | |||
Glycosylation | 306 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 307 | O-linked (Man...) threonine | |||
Glycosylation | 308 | O-linked (Man...) threonine | |||
Glycosylation | 311 | O-linked (Man...) threonine | |||
Glycosylation | 314 | O-linked (Man...) serine | |||
Glycosylation | 315 | O-linked (Man...) threonine | |||
Glycosylation | 316 | O-linked (Man...) threonine | |||
Glycosylation | 329 | O-linked (Man...) threonine | |||
Glycosylation | 331 | O-linked (Man...) serine | |||
Glycosylation | 334 | O-linked (Man...) serine | |||
Glycosylation | 335 | O-linked (Man...) serine | |||
Glycosylation | 338 | O-linked (Man...) serine | |||
Glycosylation | 339 | O-linked (Man...) threonine | |||
Glycosylation | 340 | O-linked (Man...) threonine | |||
Glycosylation | 341 | O-linked (Man...) threonine | |||
Glycosylation | 342 | O-linked (Man...) threonine | |||
Glycosylation | 345 | O-linked (Man...) threonine | |||
Glycosylation | 346 | O-linked (Man...) serine | |||
Glycosylation | 349 | O-linked (Man...) threonine | |||
Glycosylation | 350 | O-linked (Man...) serine | |||
Glycosylation | 364 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 402 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 485 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 501 | N-linked (GlcNAc...) asparagine | |||
Glycosylation | 614 | N-linked (GlcNAc...) asparagine | |||
Lipidation | 627 | GPI-anchor amidated glycine | |||
Propeptide | PRO_0000022266 | 628-650 | Removed in mature form | ||
Post-translational modification
N-glycosylated, and O-glycosylated by both PMT1 and PMT2.
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
By exposition to pheromone (A-factor) secreted by the opposite mating type cells (type A).
Structure
Family & Domains
Features
Showing features for region, repeat.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 216-322 | Ig-like fold domain important for alpha-agglutinin activity, contributing to a functional binding site for a-agglutinin | |||
Repeat | 339-378 | 1 | |||
Region | 339-423 | 2 X 40 AA tandem repeats | |||
Repeat | 384-423 | 2 | |||
Sequence similarities
To C.albicans ALS1.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length650
- Mass (Da)70,340
- Last updated1995-11-01 v2
- Checksum8BBF7A1C44C93C2B
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 449 | in Ref. 1; AAA34417 | |||
Sequence conflict | 556 | in Ref. 1; AAA34417 | |||
Sequence conflict | 581 | in Ref. 1; AAA34417 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X16861 EMBL· GenBank· DDBJ | CAA34752.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M28164 EMBL· GenBank· DDBJ | AAA34417.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X87611 EMBL· GenBank· DDBJ | CAA60926.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z49504 EMBL· GenBank· DDBJ | CAA89526.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006943 EMBL· GenBank· DDBJ | DAA08794.1 EMBL· GenBank· DDBJ | Genomic DNA |