P20789 · NTR1_RAT
- ProteinNeurotensin receptor type 1
- GeneNtsr1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids424 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
G-protein coupled receptor for the tridecapeptide neurotensin (NTS) (PubMed:23051748, PubMed:24453215, PubMed:26205105).
Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis (By similarity).
Signaling is effected via G proteins that activate a phosphatidylinositol-calcium second messenger system. Signaling leads to the activation of downstream MAP kinases and protects cells against apoptosis (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNeurotensin receptor type 1
- Short namesNT-R-1; NTR1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionP20789
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Note: Palmitoylation is required for localization at CAV1-enriched membrane rafts.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-68 | Extracellular | ||||
Sequence: MHLNSSVPQGTPGEPDAQPFSGPQSEMEATFLALSLSNGSGNTSESDTAGPNSDLDVNTDIYSKVLVT | ||||||
Transmembrane | 69-89 | Helical; Name=1 | ||||
Sequence: AIYLALFVVGTVGNSVTAFTL | ||||||
Topological domain | 90-103 | Cytoplasmic | ||||
Sequence: ARKKSLQSLQSTVH | ||||||
Transmembrane | 104-123 | Helical; Name=2 | ||||
Sequence: YHLGSLALSDLLILLLAMPV | ||||||
Topological domain | 124-143 | Extracellular | ||||
Sequence: ELYNFIWVHHPWAFGDAGCR | ||||||
Transmembrane | 144-165 | Helical; Name=3 | ||||
Sequence: GYYFLRDACTYATALNVASLSV | ||||||
Topological domain | 166-185 | Cytoplasmic | ||||
Sequence: ERYLAICHPFKAKTLMSRSR | ||||||
Transmembrane | 186-206 | Helical; Name=4 | ||||
Sequence: TKKFISAIWLASALLAIPMLF | ||||||
Topological domain | 207-235 | Extracellular | ||||
Sequence: TMGLQNRSGDGTHPGGLVCTPIVDTATVK | ||||||
Transmembrane | 236-260 | Helical; Name=5 | ||||
Sequence: VVIQVNTFMSFLFPMLVISILNTVI | ||||||
Topological domain | 261-308 | Cytoplasmic | ||||
Sequence: ANKLTVMVHQAAEQGRVCTVGTHNGLEHSTFNMTIEPGRVQALRHGVL | ||||||
Transmembrane | 309-330 | Helical; Name=6 | ||||
Sequence: VLRAVVIAFVVCWLPYHVRRLM | ||||||
Topological domain | 331-348 | Extracellular | ||||
Sequence: FCYISDEQWTTFLFDFYH | ||||||
Transmembrane | 349-369 | Helical; Name=7 | ||||
Sequence: YFYMLTNALFYVSSAINPILY | ||||||
Topological domain | 370-424 | Cytoplasmic | ||||
Sequence: NLVSANFRQVFLSTLACLCPGWRHRRKKRPTFSRKPNSMSSNHAFSTSATRETLY |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 166 | Abolishes signaling via G-proteins; when associated with A-310 and A-358. | ||||
Sequence: E → A | ||||||
Mutagenesis | 310 | Abolishes signaling via G-proteins; when associated with A-166 and A-358. | ||||
Sequence: L → A | ||||||
Mutagenesis | 358 | Abolishes signaling via G-proteins; when associated with A-166 and A-310. | ||||
Sequence: F → A |
Chemistry
PTM/Processing
Features
Showing features for chain, glycosylation, disulfide bond, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000069948 | 1-424 | Neurotensin receptor type 1 | |||
Sequence: MHLNSSVPQGTPGEPDAQPFSGPQSEMEATFLALSLSNGSGNTSESDTAGPNSDLDVNTDIYSKVLVTAIYLALFVVGTVGNSVTAFTLARKKSLQSLQSTVHYHLGSLALSDLLILLLAMPVELYNFIWVHHPWAFGDAGCRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAIPMLFTMGLQNRSGDGTHPGGLVCTPIVDTATVKVVIQVNTFMSFLFPMLVISILNTVIANKLTVMVHQAAEQGRVCTVGTHNGLEHSTFNMTIEPGRVQALRHGVLVLRAVVIAFVVCWLPYHVRRLMFCYISDEQWTTFLFDFYHYFYMLTNALFYVSSAINPILYNLVSANFRQVFLSTLACLCPGWRHRRKKRPTFSRKPNSMSSNHAFSTSATRETLY | ||||||
Glycosylation | 4 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 38 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 42 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 142↔225 | |||||
Sequence: CRGYYFLRDACTYATALNVASLSVERYLAICHPFKAKTLMSRSRTKKFISAIWLASALLAIPMLFTMGLQNRSGDGTHPGGLVC | ||||||
Lipidation | 386 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 388 | S-palmitoyl cysteine | ||||
Sequence: C |
Post-translational modification
N-glycosylated.
Palmitoylated; this is required for normal localization at membrane rafts and normal GNA11-mediated activation of down-stream signaling cascades. The palmitoylation level increases in response to neurotensin treatment.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-23 | Disordered | ||||
Sequence: MHLNSSVPQGTPGEPDAQPFSGP | ||||||
Region | 326-349 | Neurotensin binding | ||||
Sequence: VRRLMFCYISDEQWTTFLFDFYHY | ||||||
Region | 397-424 | Disordered | ||||
Sequence: KRPTFSRKPNSMSSNHAFSTSATRETLY | ||||||
Compositional bias | 403-424 | Polar residues | ||||
Sequence: RKPNSMSSNHAFSTSATRETLY |
Domain
The ligand binding pocket consists mainly of extracellular loops ECL2 and ECL3, as well as transmembrane regions TM6 and TM7.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Neurotensin receptor subfamily. NTSR1 sub-subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Protein family/group databases
Sequence
- Sequence statusComplete
- Length424
- Mass (Da)47,055
- Last updated1991-02-01 v1
- ChecksumA9C2F7EAF8D9BCD3
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 403-424 | Polar residues | ||||
Sequence: RKPNSMSSNHAFSTSATRETLY |
Keywords
- Technical term