P20676 · NUP1_YEAST
- ProteinNucleoporin NUP1
- GeneNUP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1076 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). As one of the FG repeat nucleoporins NUP1 is involved in interactions with and guidance of nuclear transport receptors such as SRP1-KAP95 (importin alpha and beta) through the NPC. Like the closely related NUP2 it also plays an important role in disassembling and recycling SRP1-KAP95 to the cytoplasm after nuclear import. Upon entry of the heterotrimeric SRP1-KAP95-cargo complex in the nucleus, NUP1 binds through its C-terminus to KAP95, thus accelerating the release of KAP95 and, indirectly, of the nuclear localization signal (NLS)-containing cargo from the SRP1-KAP95-cargo complex.
Miscellaneous
Present with 468 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nuclear envelope | |
Cellular Component | nuclear membrane | |
Cellular Component | nuclear pore | |
Cellular Component | nuclear pore central transport channel | |
Cellular Component | nuclear pore nuclear basket | |
Molecular Function | nuclear localization sequence binding | |
Molecular Function | structural constituent of nuclear pore | |
Biological Process | NLS-bearing protein import into nucleus | |
Biological Process | nucleocytoplasmic transport | |
Biological Process | poly(A)+ mRNA export from nucleus | |
Biological Process | protein import into nucleus | |
Biological Process | ribosomal large subunit export from nucleus | |
Biological Process | RNA export from nucleus | |
Biological Process | transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameNucleoporin NUP1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP20676
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Nucleus membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000204902 | 2-1076 | Nucleoporin NUP1 | |||
Sequence: SSNTSSVMSSPRVEKRSFSSTLKSFFTNPNKKRPSSKKVFSSNLSYANHLEESDVEDTLHVNKRKRVSGTSQHSDSLTQNNNNAPIIIYGTENTERPPLLPILPIQRLRLLREKQRVRNMRELGLIQSTEFPSITSSVILGSQSKSDEGGSYLCTSSTPSPIKNGSCTRQLAGKSGEDTNVGLPILKSLKNRSNRKRFHSQSKGTVWSANFEYDLSEYDAIQKKDNKDKEGNAGGDQKTSENRNNIKSSISNGNLATGPNLTSEIEDLRADINSNRLSNPQKNLLLKGPASTVAKTAPIQESFVPNSERSGTPTLKKNIEPKKDKESIVLPTVGFDFIKDNETPSKKTSPKATSSAGAVFKSSVEMGKTDKSTKTAEAPTLSFNFSQKANKTKAVDNTVPSTTLFNFGGKSDTVTSASQPFKFGKTSEKSENHTESDAPPKSTAPIFSFGKQEENGDEGDDENEPKRKRRLPVSEDTNTKPLFDFGKTGDQKETKKGESEKDASGKPSFVFGASDKQAEGTPLFTFGKKADVTSNIDSSAQFTFGKAATAKETHTKPSETPATIVKKPTFTFGQSTSENKISEGSAKPTFSFSKSEEERKSSPISNEAAKPSFSFPGKPVDVQAPTDDKTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLFSFGKSDAAKEPPGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTASTAAANTEKLSNGFSFTKFNHNKEKSNSPTSFFDGSASSTPIPVLGKPTDATGNTTSKSAFSFGTANTNGTNASANSTSFSFNAPATGNGTTTTSNTSGTNIAGTFNVGKPDQSIASGNTNGAGSAFGFSSSGTAATGAASNQSSFNFGNNGAGGLNPFTSATSSTNANAGLFNKPPSTNAQNVNVPSAFNFTGNNSTPGGGSVFNMNGNTNANTVFAGSNNQPHQSQTPSFNTNSSFTPSTVPNINFSGLNGGITNTATNALRPSDIFGANAASGSNSNVTNPSSIFGGAGGVPTTSFGQPQSAPNQMGMGTNNGMSMGGGVMANRKIARMRHSKR | ||||||
Modified residue | 54 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 161 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 381 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 383 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 637 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated by CDC28.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Component of the nuclear pore complex (NPC). NPC constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Interacts through its FG repeats with nuclear transport receptors. Binds to the nuclear basket of the NPC through NUP60. Interacts with KAP122.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P20676 | KAP95 Q06142 | 7 | EBI-12392, EBI-9145 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-39 | Disordered | ||||
Sequence: MSSNTSSVMSSPRVEKRSFSSTLKSFFTNPNKKRPSSKK | ||||||
Compositional bias | 143-176 | Polar residues | ||||
Sequence: SQSKSDEGGSYLCTSSTPSPIKNGSCTRQLAGKS | ||||||
Region | 143-183 | Disordered | ||||
Sequence: SQSKSDEGGSYLCTSSTPSPIKNGSCTRQLAGKSGEDTNVG | ||||||
Region | 224-260 | Disordered | ||||
Sequence: KKDNKDKEGNAGGDQKTSENRNNIKSSISNGNLATGP | ||||||
Compositional bias | 238-260 | Polar residues | ||||
Sequence: QKTSENRNNIKSSISNGNLATGP | ||||||
Repeat | 336-338 | FXF 1 | ||||
Sequence: FDF | ||||||
Repeat | 384-386 | FXF 2 | ||||
Sequence: FNF | ||||||
Compositional bias | 403-426 | Polar residues | ||||
Sequence: TTLFNFGGKSDTVTSASQPFKFGK | ||||||
Region | 403-518 | Disordered | ||||
Sequence: TTLFNFGGKSDTVTSASQPFKFGKTSEKSENHTESDAPPKSTAPIFSFGKQEENGDEGDDENEPKRKRRLPVSEDTNTKPLFDFGKTGDQKETKKGESEKDASGKPSFVFGASDKQ | ||||||
Repeat | 406-409 | FXFG 1 | ||||
Sequence: FNFG | ||||||
Repeat | 422-425 | FXFG 2 | ||||
Sequence: FKFG | ||||||
Repeat | 448-451 | FXFG 3 | ||||
Sequence: FSFG | ||||||
Compositional bias | 462-476 | Basic and acidic residues | ||||
Sequence: DENEPKRKRRLPVSE | ||||||
Repeat | 484-487 | FXFG 4 | ||||
Sequence: FDFG | ||||||
Compositional bias | 487-504 | Basic and acidic residues | ||||
Sequence: GKTGDQKETKKGESEKDA | ||||||
Repeat | 510-513 | FXFG 5 | ||||
Sequence: FVFG | ||||||
Repeat | 525-528 | FXFG 6 | ||||
Sequence: FTFG | ||||||
Repeat | 543-546 | FXFG 7 | ||||
Sequence: FTFG | ||||||
Compositional bias | 548-592 | Polar residues | ||||
Sequence: AATAKETHTKPSETPATIVKKPTFTFGQSTSENKISEGSAKPTFS | ||||||
Region | 548-743 | Disordered | ||||
Sequence: AATAKETHTKPSETPATIVKKPTFTFGQSTSENKISEGSAKPTFSFSKSEEERKSSPISNEAAKPSFSFPGKPVDVQAPTDDKTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLFSFGKSDAAKEPPGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTASTAA | ||||||
Repeat | 571-574 | FXFG 8 | ||||
Sequence: FTFG | ||||||
Repeat | 591-593 | FXF 3 | ||||
Sequence: FSF | ||||||
Repeat | 614-616 | FXF 4 | ||||
Sequence: FSF | ||||||
Compositional bias | 630-671 | Polar residues | ||||
Sequence: KTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLF | ||||||
Repeat | 636-638 | FXF 5 | ||||
Sequence: FSF | ||||||
Repeat | 657-659 | FXF 6 | ||||
Sequence: FTF | ||||||
Repeat | 671-674 | FXFG 9 | ||||
Sequence: FSFG | ||||||
Compositional bias | 681-743 | Polar residues | ||||
Sequence: EPPGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTASTAA | ||||||
Repeat | 689-691 | FXF 7 | ||||
Sequence: FSF | ||||||
Repeat | 708-711 | FXFG 10 | ||||
Sequence: FTFG | ||||||
Repeat | 727-730 | FXFG 11 | ||||
Sequence: FSFG | ||||||
Repeat | 753-755 | FXF 8 | ||||
Sequence: FSF | ||||||
Repeat | 800-803 | FXFG 12 | ||||
Sequence: FSFG | ||||||
Repeat | 819-821 | FXF 9 | ||||
Sequence: FSF | ||||||
Repeat | 866-868 | FXF 10 | ||||
Sequence: FGF | ||||||
Repeat | 885-888 | FXFG 13 | ||||
Sequence: FNFG | ||||||
Repeat | 929-931 | FXF 11 | ||||
Sequence: FNF | ||||||
Region | 940-979 | Disordered | ||||
Sequence: GGSVFNMNGNTNANTVFAGSNNQPHQSQTPSFNTNSSFTP | ||||||
Repeat | 1008-1009 | FG 1 | ||||
Sequence: FG | ||||||
Region | 1025-1054 | Disordered | ||||
Sequence: SIFGGAGGVPTTSFGQPQSAPNQMGMGTNN | ||||||
Repeat | 1027-1028 | FG 2 | ||||
Sequence: FG | ||||||
Repeat | 1038-1039 | FG 3 | ||||
Sequence: FG | ||||||
Region | 1040-1076 | Interaction with KAP95 | ||||
Sequence: QPQSAPNQMGMGTNNGMSMGGGVMANRKIARMRHSKR |
Domain
Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: FXFG repeats are especially abundant in NUPs on the nucleoplasmic side (in a highly charged environment and enriched in Ser and Thr).
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,076
- Mass (Da)113,581
- Last updated1991-02-01 v1
- Checksum4AC23567D2FB53CC
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 143-176 | Polar residues | ||||
Sequence: SQSKSDEGGSYLCTSSTPSPIKNGSCTRQLAGKS | ||||||
Compositional bias | 238-260 | Polar residues | ||||
Sequence: QKTSENRNNIKSSISNGNLATGP | ||||||
Compositional bias | 403-426 | Polar residues | ||||
Sequence: TTLFNFGGKSDTVTSASQPFKFGK | ||||||
Compositional bias | 462-476 | Basic and acidic residues | ||||
Sequence: DENEPKRKRRLPVSE | ||||||
Compositional bias | 487-504 | Basic and acidic residues | ||||
Sequence: GKTGDQKETKKGESEKDA | ||||||
Compositional bias | 548-592 | Polar residues | ||||
Sequence: AATAKETHTKPSETPATIVKKPTFTFGQSTSENKISEGSAKPTFS | ||||||
Compositional bias | 630-671 | Polar residues | ||||
Sequence: KTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLF | ||||||
Compositional bias | 681-743 | Polar residues | ||||
Sequence: EPPGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTASTAA |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M33632 EMBL· GenBank· DDBJ | AAA34822.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X94335 EMBL· GenBank· DDBJ | CAA64020.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z75006 EMBL· GenBank· DDBJ | CAA99295.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006948 EMBL· GenBank· DDBJ | DAA10875.1 EMBL· GenBank· DDBJ | Genomic DNA |