P20676 · NUP1_YEAST

Function

function

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). As one of the FG repeat nucleoporins NUP1 is involved in interactions with and guidance of nuclear transport receptors such as SRP1-KAP95 (importin alpha and beta) through the NPC. Like the closely related NUP2 it also plays an important role in disassembling and recycling SRP1-KAP95 to the cytoplasm after nuclear import. Upon entry of the heterotrimeric SRP1-KAP95-cargo complex in the nucleus, NUP1 binds through its C-terminus to KAP95, thus accelerating the release of KAP95 and, indirectly, of the nuclear localization signal (NLS)-containing cargo from the SRP1-KAP95-cargo complex.

Miscellaneous

Present with 468 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentnuclear envelope
Cellular Componentnuclear membrane
Cellular Componentnuclear pore
Cellular Componentnuclear pore central transport channel
Cellular Componentnuclear pore nuclear basket
Molecular Functionnuclear localization sequence binding
Molecular Functionstructural constituent of nuclear pore
Biological ProcessNLS-bearing protein import into nucleus
Biological Processnucleocytoplasmic transport
Biological Processpoly(A)+ mRNA export from nucleus
Biological Processprotein import into nucleus
Biological Processribosomal large subunit export from nucleus
Biological ProcessRNA export from nucleus
Biological Processtranscription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Nucleoporin NUP1
  • Alternative names
    • Nuclear pore protein NUP1

Gene names

    • Name
      NUP1
    • ORF names
      YOR3182C
    • Ordered locus names
      YOR098C

Organism names

Accessions

  • Primary accession
    P20676
  • Secondary accessions
    • D6W2F9

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylserine
ChainPRO_00002049022-1076Nucleoporin NUP1
Modified residue54Phosphoserine
Modified residue161Phosphoserine
Modified residue381Phosphothreonine
Modified residue383Phosphoserine
Modified residue637Phosphoserine

Post-translational modification

Phosphorylated by CDC28.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Component of the nuclear pore complex (NPC). NPC constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Interacts through its FG repeats with nuclear transport receptors. Binds to the nuclear basket of the NPC through NUP60. Interacts with KAP122.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P20676KAP95 Q061427EBI-12392, EBI-9145

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, compositional bias, repeat.

TypeIDPosition(s)Description
Region1-39Disordered
Compositional bias143-176Polar residues
Region143-183Disordered
Region224-260Disordered
Compositional bias238-260Polar residues
Repeat336-338FXF 1
Repeat384-386FXF 2
Compositional bias403-426Polar residues
Region403-518Disordered
Repeat406-409FXFG 1
Repeat422-425FXFG 2
Repeat448-451FXFG 3
Compositional bias462-476Basic and acidic residues
Repeat484-487FXFG 4
Compositional bias487-504Basic and acidic residues
Repeat510-513FXFG 5
Repeat525-528FXFG 6
Repeat543-546FXFG 7
Compositional bias548-592Polar residues
Region548-743Disordered
Repeat571-574FXFG 8
Repeat591-593FXF 3
Repeat614-616FXF 4
Compositional bias630-671Polar residues
Repeat636-638FXF 5
Repeat657-659FXF 6
Repeat671-674FXFG 9
Compositional bias681-743Polar residues
Repeat689-691FXF 7
Repeat708-711FXFG 10
Repeat727-730FXFG 11
Repeat753-755FXF 8
Repeat800-803FXFG 12
Repeat819-821FXF 9
Repeat866-868FXF 10
Repeat885-888FXFG 13
Repeat929-931FXF 11
Region940-979Disordered
Repeat1008-1009FG 1
Region1025-1054Disordered
Repeat1027-1028FG 2
Repeat1038-1039FG 3
Region1040-1076Interaction with KAP95

Domain

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: FXFG repeats are especially abundant in NUPs on the nucleoplasmic side (in a highly charged environment and enriched in Ser and Thr).

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,076
  • Mass (Da)
    113,581
  • Last updated
    1991-02-01 v1
  • Checksum
    4AC23567D2FB53CC
MSSNTSSVMSSPRVEKRSFSSTLKSFFTNPNKKRPSSKKVFSSNLSYANHLEESDVEDTLHVNKRKRVSGTSQHSDSLTQNNNNAPIIIYGTENTERPPLLPILPIQRLRLLREKQRVRNMRELGLIQSTEFPSITSSVILGSQSKSDEGGSYLCTSSTPSPIKNGSCTRQLAGKSGEDTNVGLPILKSLKNRSNRKRFHSQSKGTVWSANFEYDLSEYDAIQKKDNKDKEGNAGGDQKTSENRNNIKSSISNGNLATGPNLTSEIEDLRADINSNRLSNPQKNLLLKGPASTVAKTAPIQESFVPNSERSGTPTLKKNIEPKKDKESIVLPTVGFDFIKDNETPSKKTSPKATSSAGAVFKSSVEMGKTDKSTKTAEAPTLSFNFSQKANKTKAVDNTVPSTTLFNFGGKSDTVTSASQPFKFGKTSEKSENHTESDAPPKSTAPIFSFGKQEENGDEGDDENEPKRKRRLPVSEDTNTKPLFDFGKTGDQKETKKGESEKDASGKPSFVFGASDKQAEGTPLFTFGKKADVTSNIDSSAQFTFGKAATAKETHTKPSETPATIVKKPTFTFGQSTSENKISEGSAKPTFSFSKSEEERKSSPISNEAAKPSFSFPGKPVDVQAPTDDKTLKPTFSFTEPAQKDSSVVSEPKKPSFTFASSKTSQPKPLFSFGKSDAAKEPPGSNTSFSFTKPPANETDKRPTPPSFTFGGSTTNNTTTTSTKPSFSFGAPESMKSTASTAAANTEKLSNGFSFTKFNHNKEKSNSPTSFFDGSASSTPIPVLGKPTDATGNTTSKSAFSFGTANTNGTNASANSTSFSFNAPATGNGTTTTSNTSGTNIAGTFNVGKPDQSIASGNTNGAGSAFGFSSSGTAATGAASNQSSFNFGNNGAGGLNPFTSATSSTNANAGLFNKPPSTNAQNVNVPSAFNFTGNNSTPGGGSVFNMNGNTNANTVFAGSNNQPHQSQTPSFNTNSSFTPSTVPNINFSGLNGGITNTATNALRPSDIFGANAASGSNSNVTNPSSIFGGAGGVPTTSFGQPQSAPNQMGMGTNNGMSMGGGVMANRKIARMRHSKR

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias143-176Polar residues
Compositional bias238-260Polar residues
Compositional bias403-426Polar residues
Compositional bias462-476Basic and acidic residues
Compositional bias487-504Basic and acidic residues
Compositional bias548-592Polar residues
Compositional bias630-671Polar residues
Compositional bias681-743Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M33632
EMBL· GenBank· DDBJ
AAA34822.1
EMBL· GenBank· DDBJ
Genomic DNA
X94335
EMBL· GenBank· DDBJ
CAA64020.1
EMBL· GenBank· DDBJ
Genomic DNA
Z75006
EMBL· GenBank· DDBJ
CAA99295.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006948
EMBL· GenBank· DDBJ
DAA10875.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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