P20485 · KICH_YEAST

Function

function

Catalyzes the committed step in the synthesis of phosphatidylcholine by the CDP-choline pathway (PubMed:10329685, PubMed:2536698, PubMed:9506987).
Also exhibits ethanolamine kinase activity but it is a poor substrate at 14% efficiency compared with choline (PubMed:2536698, PubMed:9506987).

Miscellaneous

Present with 3930 molecules/cell in log phase SD medium.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
90 μMATP
0.27 mMcholine
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
138.7 μmol/min/mgfor choline

pH Dependence

Optimum pH is 9.5-12.

Temperature Dependence

Optimum temperature is 30 degrees Celsius.

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from choline: step 1/1.

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functioncholine kinase activity
Molecular Functionethanolamine kinase activity
Biological Processphosphatidylcholine biosynthetic process
Biological Processphosphatidylethanolamine biosynthetic process
Biological Processphosphorylation

Keywords

Enzyme and pathway databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    Choline kinase
  • EC number
  • Alternative names
    • ATP:choline phosphotransferase

Gene names

    • Name
      CKI1
    • Synonyms
      CKI
    • ORF names
      L3130, L9606.8
    • Ordered locus names
      YLR133W

Organism names

Accessions

  • Primary accession
    P20485
  • Secondary accessions
    • D6VYC8

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis30Decrease in activity and in phosphorylation by PKA. No phosphorylation by PKA, and strong decrease in activity; when associated with A-85.
Mutagenesis85No phosphorylation by PKA, and strong decrease in activity; when associated with A-30.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 10 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for initiator methionine, chain, modified residue.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00002062262-582Choline kinase
Modified residue30Phosphoserine; by PKA
Modified residue48Phosphoserine
Modified residue51Phosphoserine
Modified residue54Phosphothreonine
Modified residue85Phosphoserine; by PKA

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Monomer. Interacts with NAP1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P20485NAP1 P252935EBI-9699, EBI-11850

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias1-25Polar residues
Region1-36Disordered

Sequence similarities

Belongs to the choline/ethanolamine kinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    582
  • Mass (Da)
    66,317
  • Last updated
    1991-02-01 v1
  • Checksum
    68C395B9CC120A0E
MVQESRPGSVRSYSVGYQARSRSSSQRRHSLTRQRSSQRLIRTISIESDVSNITDDDDLRAVNEGVAGVQLDVSETANKGPRRASATDVTDSLGSTSSEYIEIPFVKETLDASLPSDYLKQDILNLIQSLKISKWYNNKKIQPVAQDMNLVKISGAMTNAIFKVEYPKLPSLLLRIYGPNIDNIIDREYELQILARLSLKNIGPSLYGCFVNGRFEQFLENSKTLTKDDIRNWKNSQRIARRMKELHVGVPLLSSERKNGSACWQKINQWLRTIEKVDQWVGDPKNIENSLLCENWSKFMDIVDRYHKWLISQEQGIEQVNKNLIFCHNDAQYGNLLFTAPVMNTPSLYTAPSSTSLTSQSSSLFPSSSNVIVDDIINPPKQEQSQDSKLVVIDFEYAGANPAAYDLANHLSEWMYDYNNAKAPHQCHADRYPDKEQVLNFLYSYVSHLRGGAKEPIDEEVQRLYKSIIQWRPTVQLFWSLWAILQSGKLEKKEASTAITREEIGPNGKKYIIKTEPESPEEDFVENDDEPEAGVSIDTFDYMAYGRDKIAVFWGDLIGLGIITEEECKNFSSFKFLDTSYL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-25Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
J04454
EMBL· GenBank· DDBJ
AAA34499.1
EMBL· GenBank· DDBJ
Genomic DNA
X91258
EMBL· GenBank· DDBJ
CAA62646.1
EMBL· GenBank· DDBJ
Genomic DNA
Z73305
EMBL· GenBank· DDBJ
CAA97704.1
EMBL· GenBank· DDBJ
Genomic DNA
U53881
EMBL· GenBank· DDBJ
AAB82396.1
EMBL· GenBank· DDBJ
Genomic DNA
AY692779
EMBL· GenBank· DDBJ
AAT92798.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006945
EMBL· GenBank· DDBJ
DAA09444.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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