P20448 · DBP4_YEAST
- ProteinATP-dependent RNA helicase HCA4
- GeneHCA4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids770 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
ATP-dependent RNA helicase required for ribosome biogenesis. Involved in the release of U14 snoRNA in pre-ribosomal complexes. Required for pre-rRNA cleavage at site A2.
Miscellaneous
Present with 7900 molecules/cell in log phase SD medium.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.24 mM | ATP |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleolus | |
Cellular Component | nucleus | |
Cellular Component | small-subunit processome | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent activity, acting on RNA | |
Molecular Function | identical protein binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Biological Process | maturation of SSU-rRNA | |
Biological Process | rRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent RNA helicase HCA4
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP20448
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 91 | Lethal and prevents release of U14 snoRNA from pre-ribosomes. | ||||
Sequence: K → A or R | ||||||
Mutagenesis | 225 | Lethal and prevents release of U14 snoRNA from pre-ribosomes; when associated with A-227. | ||||
Sequence: S → A | ||||||
Mutagenesis | 227 | Lethal and prevents release of U14 snoRNA from pre-ribosomes; when associated with A-225. | ||||
Sequence: T → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000055018 | 1-770 | ATP-dependent RNA helicase HCA4 | |||
Sequence: MAKKNRLNTTQRKTLRQKEDEYIENLKTKIDEYDPKITKAKFFKDLPISDPTLKGLRESSFIKLTEIQADSIPVSLQGHDVLAAAKTGSGKTLAFLVPVIEKLYREKWTEFDGLGALIISPTRELAMQIYEVLTKIGSHTSFSAGLVIGGKDVKFELERISRINILIGTPGRILQHLDQAVGLNTSNLQMLVLDEADRCLDMGFKKTLDAIVSTLSPSRQTLLFSATQSQSVADLARLSLTDYKTVGTHDVMDGSVNKEASTPETLQQFYIEVPLADKLDILFSFIKSHLKCKMIVFLSSSKQVHFVYETFRKMQPGISLMHLHGRQKQRARTETLDKFNRAQQVCLFATDVVARGIDFPAVDWVVQVDCPEDVDTYIHRVGRCARYGKKGKSLIMLTPQEQEAFLKRLNARKIEPGKLNIKQSKKKSIKPQLQSLLFKDPELKYLGQKAFISYVRSIYVQKDKQVFKFDELPTEEFAYSLGLPGAPKIKMKGMKTIEQAKERKNAPRQLAFLSKANEDGEVIEDKSKQPRTKYDKMFERKNQTILSEHYLNITKAQAQEDEDDDFISVKRKDHEINEAELPALTLPTSRRAQKKALSKKASLASKGNASKLIFDDEGEAHPVYELEDEEEFHKRGDAEVQKTEFLTKESAVMADIDNIDKQVAKEKKQEKKRKRLEAMRREMEAAMEEEISGDEEEGKTVAYLGTGNLSDDMSDGDMPDSEGHLKKKARTVDYSHGHNPSNSVDDDIIEVEEPQTLEDLESLTAKLIQG | ||||||
Modified residue | 692 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 710 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 714 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 743 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with the U3 and U14 snoRNAs. Associates with pre-ribosomal complexes.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P20448 | BFR2 Q06631 | 6 | EBI-5612, EBI-36432 | |
BINARY | P20448 | CKA1 P15790 | 2 | EBI-5612, EBI-9533 | |
BINARY | P20448 | ENP2 P48234 | 4 | EBI-5612, EBI-23354 | |
BINARY | P20448 | ESF1 Q06344 | 4 | EBI-5612, EBI-34121 | |
BINARY | P20448 | ESF2 P53743 | 3 | EBI-5612, EBI-28537 | |
BINARY | P20448 | HAS1 Q03532 | 3 | EBI-5612, EBI-8170 | |
BINARY | P20448 | HCA4 P20448 | 2 | EBI-5612, EBI-5612 | |
BINARY | P20448 | RRP36 Q12481 | 3 | EBI-5612, EBI-31770 | |
BINARY | P20448 | SAS10 Q12136 | 4 | EBI-5612, EBI-36084 | |
BINARY | P20448 | UTP18 P40362 | 3 | EBI-5612, EBI-4534 | |
BINARY | P20448 | UTP22 P53254 | 4 | EBI-5612, EBI-1878 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 41-69 | Q motif | ||||
Sequence: KFFKDLPISDPTLKGLRESSFIKLTEIQA | ||||||
Domain | 72-246 | Helicase ATP-binding | ||||
Sequence: IPVSLQGHDVLAAAKTGSGKTLAFLVPVIEKLYREKWTEFDGLGALIISPTRELAMQIYEVLTKIGSHTSFSAGLVIGGKDVKFELERISRINILIGTPGRILQHLDQAVGLNTSNLQMLVLDEADRCLDMGFKKTLDAIVSTLSPSRQTLLFSATQSQSVADLARLSLTDYKTV | ||||||
Motif | 194-197 | DEAD box | ||||
Sequence: DEAD | ||||||
Domain | 278-437 | Helicase C-terminal | ||||
Sequence: KLDILFSFIKSHLKCKMIVFLSSSKQVHFVYETFRKMQPGISLMHLHGRQKQRARTETLDKFNRAQQVCLFATDVVARGIDFPAVDWVVQVDCPEDVDTYIHRVGRCARYGKKGKSLIMLTPQEQEAFLKRLNARKIEPGKLNIKQSKKKSIKPQLQSLL | ||||||
Region | 705-724 | Disordered | ||||
Sequence: GTGNLSDDMSDGDMPDSEGH |
Domain
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities
Belongs to the DEAD box helicase family. DDX10/DBP4 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length770
- Mass (Da)87,193
- Last updated1995-11-01 v2
- Checksum0C9E4DEF5BFD640E
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 36 | in Ref. 4; AAT93137 | ||||
Sequence: K → R | ||||||
Sequence conflict | 381 | in Ref. 5; no nucleotide entry | ||||
Sequence: V → I | ||||||
Sequence conflict | 465 | in Ref. 1; AAB17005 | ||||
Sequence: Q → E | ||||||
Sequence conflict | 512 | in Ref. 1; AAB17005 | ||||
Sequence: F → L | ||||||
Sequence conflict | 668-673 | in Ref. 1; AAB17005 | ||||
Sequence: KQEKKR → NKRRRG | ||||||
Sequence conflict | 675 | in Ref. 1; AAB17005 | ||||
Sequence: R → D |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U72149 EMBL· GenBank· DDBJ | AAB17005.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z49308 EMBL· GenBank· DDBJ | CAA89324.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY693118 EMBL· GenBank· DDBJ | AAT93137.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006943 EMBL· GenBank· DDBJ | DAA08766.1 EMBL· GenBank· DDBJ | Genomic DNA |