P20432 · GSTD1_DROME
- ProteinGlutathione S-transferase D1
- GeneGstD1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids209 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:20417639, PubMed:22082028).
Has DDT dehydrochlorinase activity (PubMed:20417639).
May be involved in detoxification (PubMed:22082028).
Has DDT dehydrochlorinase activity (PubMed:20417639).
May be involved in detoxification (PubMed:22082028).
Miscellaneous
Has a specific activity toward 1-chloro-2,4-dinitrobenzene comparable to that for the mammalian glutathione S-transferases but did not have as broad a substrate specificity pattern. Has no GSH peroxidase activity.
Catalytic activity
- glutathione + RX = a halide anion + an S-substituted glutathione + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.23 mM | glutathione | |||||
0.45 mM | 1-chloro-2,4-dinitrobenzene |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
84.2 μmol/min/mg | with 1-chloro-2,4-dinitrobenzene as substrate | ||||
7.51 μmol/min/mg | with 4-hydroxy-2-nonenal as substrate | ||||
77 nmol/min/mg | with adrenochrome as substrate | ||||
0.33 μmol/min/mg | with phenethyl isothiocyanate as substrate | ||||
0.517 μmol/min/mg | with prostaglandin A2 as substrate | ||||
0.012 μmol/min/mg | with 5-hydroperoxyeicosatetraenoic acid as substrate | ||||
0.22 μmol/min/mg | with 2-hydroxyethyl disulfide as substrate |
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | DDT-dehydrochlorinase activity | |
Molecular Function | glutathione peroxidase activity | |
Molecular Function | glutathione transferase activity | |
Biological Process | glutathione metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutathione S-transferase D1
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionP20432
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000185947 | 1-209 | Glutathione S-transferase D1 | |||
Sequence: MVDFYYLPGSSPCRSVIMTAKAVGVELNKKLLNLQAGEHLKPEFLKINPQHTIPTLVDNGFALWESRAIQVYLVEKYGKTDSLYPKCPKKRAVINQRLYFDMGTLYQSFANYYYPQVFAKAPADPEAFKKIEAAFEFLNTFLEGQDYAAGDSLTVADIALVATVSTFEVAKFEISKYANVNRWYENAKKVTPGWEENWAGCLEFKKYFE |
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Homodimer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P20432 | GstD10 Q9VGA1 | 4 | EBI-97726, EBI-149969 | |
BINARY | P20432 | GstD8 Q9VG92 | 4 | EBI-97726, EBI-15116229 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-81 | GST N-terminal | ||||
Sequence: MVDFYYLPGSSPCRSVIMTAKAVGVELNKKLLNLQAGEHLKPEFLKINPQHTIPTLVDNGFALWESRAIQVYLVEKYGKTD | ||||||
Domain | 87-208 | GST C-terminal | ||||
Sequence: CPKKRAVINQRLYFDMGTLYQSFANYYYPQVFAKAPADPEAFKKIEAAFEFLNTFLEGQDYAAGDSLTVADIALVATVSTFEVAKFEISKYANVNRWYENAKKVTPGWEENWAGCLEFKKYF |
Sequence similarities
Belongs to the GST superfamily. Delta family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length209
- Mass (Da)23,866
- Last updated1991-02-01 v1
- Checksum4AD8E237CCF804F2
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X14233 EMBL· GenBank· DDBJ | CAA32449.1 EMBL· GenBank· DDBJ | mRNA | ||
S51044 EMBL· GenBank· DDBJ | AAA04220.1 EMBL· GenBank· DDBJ | mRNA | ||
M97702 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AE014297 EMBL· GenBank· DDBJ | AAF54786.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014297 EMBL· GenBank· DDBJ | ABC66168.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY121705 EMBL· GenBank· DDBJ | AAM52032.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |