P20311 · DPOL_BPT3
- ProteinDNA-directed DNA polymerase
- Gene5
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids704 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Replicates viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'-5' direction.
Catalytic activity
- a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)
Cofactor
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 5 | Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity | ||||
Sequence: D | ||||||
Binding site | 5 | Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity | ||||
Sequence: D | ||||||
Binding site | 7 | Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity | ||||
Sequence: E | ||||||
Binding site | 174 | Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity | ||||
Sequence: D | ||||||
Binding site | 475 | Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity | ||||
Sequence: D | ||||||
Binding site | 475 | Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity | ||||
Sequence: D | ||||||
Binding site | 476 | Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity | ||||
Sequence: A | ||||||
Binding site | 506 | substrate | ||||
Sequence: H | ||||||
Binding site | 518 | substrate | ||||
Sequence: R | ||||||
Binding site | 522 | substrate | ||||
Sequence: K | ||||||
Binding site | 526 | substrate | ||||
Sequence: Y | ||||||
Binding site | 654 | Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity | ||||
Sequence: D | ||||||
Binding site | 654 | Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 3'-5' exonuclease activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed DNA polymerase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Biological Process | DNA-templated DNA replication | |
Biological Process | double-strand break repair | |
Biological Process | viral DNA genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed DNA polymerase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Autographiviridae > Studiervirinae > Teetrevirus > Teetrevirus T3Luria
- Virus hosts
Accessions
- Primary accessionP20311
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000101268 | 1-704 | DNA-directed DNA polymerase | |||
Sequence: MLVSDIEANNLLEKVTKFHCGVIYDYRDGEYHSYRPGDFGAYLDALEAEVKRGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHSNLKDTDMGLLRSGKLPGKRFGSHALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVDGMEWWNFNEEMMDYNVQDVVVTKALLEKLLSDKHYFPPEIDFTDVGYTTFWSESLEAVDVEHRAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRNLTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKIPKVGGIFKKPKNKAQREGREPCELDTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKFTDKGAPVVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLDGITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQMAAELPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAIAALRESIQQTLVESSQWVAGEQQVKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWIHDEIQVACRTEEIAKTVIEVAQEAMRWVGEHWNFRCLLDTEGKMGANWKECH |
Interaction
Subunit
Composed of two subunits. One is encoded by the phage and the other is encoded by the host thioredoxin.
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-187 | 3'-5'exonuclease | ||||
Sequence: MLVSDIEANNLLEKVTKFHCGVIYDYRDGEYHSYRPGDFGAYLDALEAEVKRGGLIVFHNGHKYDVPALTKLAKLQLNREFHLPRENCIDTLVLSRLIHSNLKDTDMGLLRSGKLPGKRFGSHALEAWGYRLGEMKGEYKDDFKRMLEEQGEEYVDGMEWWNFNEEMMDYNVQDVVVTKALLEKLLS | ||||||
Region | 202-704 | Polymerase | ||||
Sequence: GYTTFWSESLEAVDVEHRAAWLLAKQERNGFPFDTKAIEELYVELAARRSELLRNLTETFGSWYQPKGGTEMFCHPRTGKPLPKYPRIKIPKVGGIFKKPKNKAQREGREPCELDTREYVAGAPYTPVEHVVFNPSSRDHIQKKLQEAGWVPTKFTDKGAPVVDDEVLEGVRVDDPEKQAAIDLIKEYLMIQKRIGQSAEGDKAWLRYVAEDGKIHGSVNPNGAVTGRATHAFPNLAQIPGVRSPYGEQCRAAFGAEHHLDGITGKPWVQAGIDASGLELRCLAHFMARFDNGEYAHEILNGDIHTKNQMAAELPTRDNAKTFIYGFLYGAGDEKIGQIVGAGKERGKELKKKFLENTPAIAALRESIQQTLVESSQWVAGEQQVKWKRRWIKGLDGRKVHVRSPHAALNTLLQSAGALICKLWIIKTEEMLVEKGLKHGWDGDFAYMAWIHDEIQVACRTEEIAKTVIEVAQEAMRWVGEHWNFRCLLDTEGKMGANWKECH |
Sequence similarities
Belongs to the DNA polymerase type-A family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length704
- Mass (Da)79,985
- Last updated1991-02-01 v1
- ChecksumD65DFBD99AE31234
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X17255 EMBL· GenBank· DDBJ | CAA35140.1 EMBL· GenBank· DDBJ | Genomic DNA |