P18754 · RCC1_HUMAN

  • Protein
    Regulator of chromosome condensation
  • Gene
    RCC1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:11336674, PubMed:17435751, PubMed:1944575, PubMed:20668449, PubMed:22215983, PubMed:29042532).
Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983).
Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983).
Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831).
Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580).

Miscellaneous

Patients with Raynaud disease produce antibodies that bind to RCC1.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentchromatin
Cellular Componentchromosome
Cellular Componentcondensed nuclear chromosome
Cellular Componentcytoplasm
Cellular Componentnucleoplasm
Cellular Componentnucleus
Cellular Componentprotein-containing complex
Molecular Functionchromatin binding
Molecular Functionguanyl-nucleotide exchange factor activity
Molecular Functionhistone binding
Molecular Functionnucleosomal DNA binding
Molecular Functionnucleosome binding
Molecular Functionprotein heterodimerization activity
Molecular Functionsmall GTPase binding
Molecular Functionsulfate binding
Biological Processcell division
Biological Processchromosome segregation
Biological ProcessG1/S transition of mitotic cell cycle
Biological Processmitotic nuclear membrane reassembly
Biological Processmitotic spindle organization
Biological Processregulation of mitotic nuclear division
Biological Processspindle assembly
Biological Processviral process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Regulator of chromosome condensation
  • Alternative names
    • Cell cycle regulatory protein
    • Chromosome condensation protein 1

Gene names

    • Name
      RCC1
    • Synonyms
      CHC1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    P18754
  • Secondary accessions
    • Q16269
    • Q6NT97

Proteomes

Organism-specific databases

Subcellular Location

Nucleus
Chromosome
Cytoplasm
Note: Predominantly nuclear in interphase cells (PubMed:12194828).
Binds to mitotic chromosomes (PubMed:12194828, PubMed:17435751, PubMed:20668449).

Keywords

Disease & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis2Does not abolish N-terminal methylation.
Mutagenesis2Does not abolish N-terminal methylation.
Mutagenesis3Abolishes N-terminal methylation.
Mutagenesis4Abolishes N-terminal methylation.
Mutagenesis4Strongly impairs N-terminal methylation and subcellular localization.
Mutagenesis9Decreases KPNA4 binding. Strongly decreases KPNA4 binding; when associated with A-21.
Mutagenesis11Phosphomimetic mutant. Decreases KPNA4 binding by about 10%.
Mutagenesis21Decreases KPNA4 binding. Strongly decreases KPNA4 binding; when associated with A-9.
Mutagenesis182Abolishes interaction with Ran and impairs chromosome localization.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 431 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Organism-specific databases

Miscellaneous

Genetic variation databases

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain, modified residue (large scale data).

TypeIDPosition(s)SourceDescription
Initiator methionine1UniProtRemoved
Modified residue2UniProtN,N,N-trimethylserine; alternate
Modified residue2UniProtN,N-dimethylserine; alternate
Modified residue2UniProtN-methylserine; alternate
Modified residue2UniProtPhosphoserine
ChainPRO_00002066282-421UniProtRegulator of chromosome condensation
Modified residue11UniProtPhosphoserine
Modified residue (large scale data)11PRIDEPhosphoserine
Modified residue (large scale data)104PRIDEPhosphoserine

Post-translational modification

N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Organism-specific databases

Interaction

Subunit

Interacts with RAN (PubMed:11336674, PubMed:17435751, PubMed:18762580, PubMed:29040603).
Interacts with KPNA3 (PubMed:34564892).
Interacts (via N-terminus and RCC1 repeats) with KPNA4 (PubMed:29042532).
Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation (PubMed:16820410).

Binary interactions

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, motif, compositional bias, repeat.

TypeIDPosition(s)Description
Region1-36Disordered
Motif4-24Bipartite nuclear localization signal
Compositional bias8-22Basic and acidic residues
Repeat34-84RCC1 1
Repeat85-136RCC1 2
Repeat138-189RCC1 3
Repeat191-257RCC1 4
Repeat258-311RCC1 5
Repeat312-362RCC1 6
Repeat363-416RCC1 7

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

P18754-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    421
  • Mass (Da)
    44,969
  • Last updated
    1990-11-01 v1
  • Checksum
    F6D225AF81928305
MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS

P18754-2

  • Name
    2
  • Synonyms
    RCC1-I
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 24-24: K → KDTRAAASRRVPGARSCQGACGPSPPDQKTRP

Computationally mapped potential isoform sequences

There are 6 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
C9J3R0C9J3R0_HUMANRCC1264
C9JW69C9JW69_HUMANRCC1372
C9JRH2C9JRH2_HUMANRCC1230
C9JQZ4C9JQZ4_HUMANRCC1283
C9JMJ4C9JMJ4_HUMANRCC1272
M0R1Q8M0R1Q8_HUMANRCC1148

Features

Showing features for compositional bias, alternative sequence.

TypeIDPosition(s)Description
Compositional bias8-22Basic and acidic residues
Alternative sequenceVSP_04112224in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X12654
EMBL· GenBank· DDBJ
CAA31182.1
EMBL· GenBank· DDBJ
mRNA
X06130
EMBL· GenBank· DDBJ
CAA29496.1
EMBL· GenBank· DDBJ
mRNA
D00591
EMBL· GenBank· DDBJ
BAA00469.1
EMBL· GenBank· DDBJ
Genomic DNA
AF498924
EMBL· GenBank· DDBJ
AAM21072.1
EMBL· GenBank· DDBJ
mRNA
BC007300
EMBL· GenBank· DDBJ
AAH07300.1
EMBL· GenBank· DDBJ
mRNA
BC010067
EMBL· GenBank· DDBJ
AAH10067.1
EMBL· GenBank· DDBJ
mRNA
BC036903
EMBL· GenBank· DDBJ
AAH36903.1
EMBL· GenBank· DDBJ
mRNA
BC069198
EMBL· GenBank· DDBJ
AAH69198.1
EMBL· GenBank· DDBJ
mRNA
S75708
EMBL· GenBank· DDBJ
AAB32653.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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