P18478 · POLG_WMV2U
- ProteinGenome polyprotein
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1017 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Nuclear inclusion protein A
Has RNA-binding and proteolytic activities.
Nuclear inclusion protein B
An RNA-dependent RNA polymerase that plays an essential role in the virus replication.
Capsid protein
Involved in aphid transmission, cell-to-cell and systemis movement, encapsidation of the viral RNA and in the regulation of viral RNA amplification.
Miscellaneous
Readthrough of a terminator codon TGA occurs between codons for Ala-267 and Gln-269.
Catalytic activity
- Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biotechnology
The gene for the coat protein is introduced by genetic manipulation and expressed in squash so as to obtain virus resistant plants.
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 57 | For nuclear inclusion protein A activity | ||||
Sequence: D | ||||||
Active site | 127 | For nuclear inclusion protein A activity | ||||
Sequence: C | ||||||
Site | 219-220 | Cleavage; by NIa-pro | ||||
Sequence: QS | ||||||
Site | 736-737 | Cleavage; by NIa-pro | ||||
Sequence: QS |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | viral capsid | |
Molecular Function | cysteine-type peptidase activity | |
Molecular Function | nucleotide binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | DNA-templated transcription | |
Biological Process | proteolysis | |
Biological Process | viral RNA genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameGenome polyprotein
- Cleaved into 3 chains
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Pisuviricota > Stelpaviricetes > Patatavirales > Potyviridae > Potyvirus > Watermelon mosaic virus
- Virus hosts
Accessions
- Primary accessionP18478
Subcellular Location
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000040497 | 1-219 | Nuclear inclusion protein A | |||
Sequence: LSVTRKQCLELGMVLSLSPMGTYLEGTMGCLQLKHGHGGFVIHNTTQLRIHFIQGKDAILIRMPKIFLRLQSATSLDNQNVRNEFAWLEQTFKRRAYGQQSQSSSIILPEGKGSFWIHWITTQDGFCGLPLVSVNDGHVVGIHGLTSNDSEKNFFVPFTDGFEKEYLDNADNLSWDKHWFWEPSKIAWGPLNLVEEQPKEEFKISKLVSDLFGNTVAVQ | ||||||
Chain | PRO_0000420034 | 1-1017 | Genome polyprotein | |||
Sequence: LSVTRKQCLELGMVLSLSPMGTYLEGTMGCLQLKHGHGGFVIHNTTQLRIHFIQGKDAILIRMPKIFLRLQSATSLDNQNVRNEFAWLEQTFKRRAYGQQSQSSSIILPEGKGSFWIHWITTQDGFCGLPLVSVNDGHVVGIHGLTSNDSEKNFFVPFTDGFEKEYLDNADNLSWDKHWFWEPSKIAWGPLNLVEEQPKEEFKISKLVSDLFGNTVAVQSRKERWVLDAMEGNLVACGQADSALVTKHVVKGKCPYFAQYLSLHDGAXQFFEPLMGAYQPSRLNKDAFKKDFFKYNKPVVLNEVDFNAFEKAVEGVITMMVDFEFAECLFVTDPDEIYGSLNMKAAVGAQYKGKKQDYFSGMDSFDKERLLYLSCERLFNGEKGIWNGSLKAELRPIEKVQANKTRTFTAAPLDTLLGAKVCVDDFNNQFYSFNLKCPWTVGMTKFYGGWDKLMRSLPDGWTYCHADGSQFDSSLTPLLLNAVLSIRCCFMEDWWVGREMLENLYAEIVYTPILAPDGTIFKKFRGNNSGQPSTVVDNTLMVVIAMYYSCCKQGWSEEDIERRLVFFANGDDIILAVKDEDVWLYDTLSASFAELGLNYNFDERTKKREELWFMSHQAMLVDGIYIPKLEPERIVSILEWDRSKELMHRTEAICAAMIEAWGYTELLQEIRKFYLWLLSKDEFKELAASGKAPYIAETALRKLYTDVNTQPSELQRYLEVLDFNHIDGCCESVSLQSGKETVENLDAGKESKKDASDKGNKPQNSQVGQGSKEPTKTGTVSKDVNVGSKGKEVPRLQKITKKMNLPTVGGKIILSLDHLLEYKPSQVDLFNTRATKTQFESWYSAVKVEYDLNDEQMGVIMNGFMVWCIDNGTSPDVNGVWVMMDGEEQVEYPLKPIVENAKPTLRQIMHHFSDAAEAYIEMRNSESPYMPRYGLLRNLRDRELARYAFDFYEVTSKTPNRAREAIAQMKAAALAGVNSRLFGLDGNISTNSENTGRHTARDVNQNMHTLLGMGPPQ | ||||||
Chain | PRO_0000040498 | 220-736 | Nuclear inclusion protein B | |||
Sequence: SRKERWVLDAMEGNLVACGQADSALVTKHVVKGKCPYFAQYLSLHDGAXQFFEPLMGAYQPSRLNKDAFKKDFFKYNKPVVLNEVDFNAFEKAVEGVITMMVDFEFAECLFVTDPDEIYGSLNMKAAVGAQYKGKKQDYFSGMDSFDKERLLYLSCERLFNGEKGIWNGSLKAELRPIEKVQANKTRTFTAAPLDTLLGAKVCVDDFNNQFYSFNLKCPWTVGMTKFYGGWDKLMRSLPDGWTYCHADGSQFDSSLTPLLLNAVLSIRCCFMEDWWVGREMLENLYAEIVYTPILAPDGTIFKKFRGNNSGQPSTVVDNTLMVVIAMYYSCCKQGWSEEDIERRLVFFANGDDIILAVKDEDVWLYDTLSASFAELGLNYNFDERTKKREELWFMSHQAMLVDGIYIPKLEPERIVSILEWDRSKELMHRTEAICAAMIEAWGYTELLQEIRKFYLWLLSKDEFKELAASGKAPYIAETALRKLYTDVNTQPSELQRYLEVLDFNHIDGCCESVSLQ | ||||||
Chain | PRO_0000040499 | 737-1017 | Capsid protein | |||
Sequence: SGKETVENLDAGKESKKDASDKGNKPQNSQVGQGSKEPTKTGTVSKDVNVGSKGKEVPRLQKITKKMNLPTVGGKIILSLDHLLEYKPSQVDLFNTRATKTQFESWYSAVKVEYDLNDEQMGVIMNGFMVWCIDNGTSPDVNGVWVMMDGEEQVEYPLKPIVENAKPTLRQIMHHFSDAAEAYIEMRNSESPYMPRYGLLRNLRDRELARYAFDFYEVTSKTPNRAREAIAQMKAAALAGVNSRLFGLDGNISTNSENTGRHTARDVNQNMHTLLGMGPPQ |
Post-translational modification
Genome polyprotein of potyviruses undergoes post-translational proteolytic processing by the main proteinase NIa-pro resulting in the production of at least ten individual proteins. The P1 proteinase and the HC-pro cleave only their respective C-termini autocatalytically. 6K1 is essential for proper proteolytic separation of P3 from CI (By similarity).
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-195 | Peptidase C4 | ||||
Sequence: LSVTRKQCLELGMVLSLSPMGTYLEGTMGCLQLKHGHGGFVIHNTTQLRIHFIQGKDAILIRMPKIFLRLQSATSLDNQNVRNEFAWLEQTFKRRAYGQQSQSSSIILPEGKGSFWIHWITTQDGFCGLPLVSVNDGHVVGIHGLTSNDSEKNFFVPFTDGFEKEYLDNADNLSWDKHWFWEPSKIAWGPLNLVE | ||||||
Domain | 461-585 | RdRp catalytic | ||||
Sequence: WTYCHADGSQFDSSLTPLLLNAVLSIRCCFMEDWWVGREMLENLYAEIVYTPILAPDGTIFKKFRGNNSGQPSTVVDNTLMVVIAMYYSCCKQGWSEEDIERRLVFFANGDDIILAVKDEDVWLY | ||||||
Region | 741-787 | Disordered | ||||
Sequence: TVENLDAGKESKKDASDKGNKPQNSQVGQGSKEPTKTGTVSKDVNVG | ||||||
Compositional bias | 743-759 | Basic and acidic residues | ||||
Sequence: ENLDAGKESKKDASDKG | ||||||
Compositional bias | 760-784 | Polar residues | ||||
Sequence: NKPQNSQVGQGSKEPTKTGTVSKDV |
Sequence similarities
Belongs to the potyviridae genome polyprotein family.
Family and domain databases
Sequence
- Sequence statusFragment
- Length1,017
- Mass (Da)115,489
- Last updated2006-09-05 v3
- Checksum8853B6411C871E2E
Features
Showing features for non-terminal residue, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: L | ||||||
Compositional bias | 743-759 | Basic and acidic residues | ||||
Sequence: ENLDAGKESKKDASDKG | ||||||
Compositional bias | 760-784 | Polar residues | ||||
Sequence: NKPQNSQVGQGSKEPTKTGTVSKDV |
Keywords
- Technical term