P18431 · SGG_DROME
- ProteinProtein kinase shaggy
- Genesgg
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids514 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for several developmental events such as syncytial blastoderm formation and embryonic segmentation. Is involved in transcriptional regulation. Required for arm phosphorylation. Wg signaling operates by inactivating the sgg repression of en autoactivation. Negatively controls the neuromuscular junction (NMJ) growth in presynaptic motoneurons. Plays a role in the regulation of microtubule dynamics and actin cytoskeleton during embryogenesis. Required for phosphorylation of sra in activated eggs. Essential for completion of meiosis, possibly by triggering calcineurin activation via sra phosphorylation. Phosphorylates microtubule-associated protein futsch in axons.
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Features
Showing features for binding site, active site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein kinase shaggy
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionP18431
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: In syncytial embryos, detected at the centrosomes throughout the cell cycle, and in the mitotic spindle and pseudocleavage furrows invaginating from the cell cortex during mitosis. Concentrated at the growing end of membranes during the cellularization process. After cellularization, localized to the centrosomes during mitosis and to the nucleus at the end of telophase. Enriched in the presynaptic side of the neuromuscular junction, with some signal detected also in axonal branches.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mutants display an overdeveloped neuromuscular junction (NMJ), with the number of boutons greatly increased.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 81 | Increases the amount of synaptic boutons and microtubule loops when expressed presynaptically. | ||||
Sequence: A → T |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000086641 | 1-514 | Protein kinase shaggy | |||
Sequence: MSGRPRTSSFAEGNKQSPSLVLGGVKTCSRDGSKITTVVATPGQGTDRVQEVSYTDTKVIGNGSFGVVFQAKLCDTGELVAIKKVLQDRRFKNRELQIMRKLEHCNIVKLLYFFYSSGEKRDEVFLNLVLEYIPETVYKVARQYAKTKQTIPINFIRLYMYQLFRSLAYIHSLGICHRDIKPQNLLLDPETAVLKLCDFGSAKQLLHGEPNVSYICSRYYRAPELIFGAINYTTKIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEVIKVLGTPTREQIREMNPNYTEFKFPQIKSHPWQKVFRIRTPTEAINLVSLLLEYTPSARITPLKACAHPFFDELRMEGNHTLPNGRDMPPLFNFTEHELSIQPSLVPQLLPKHLQNASGPGGNRPSAGGAASIAASGSTSVSSTGSGASVEGSAQPQSQGTAAAAGSGSGGATAGTGGASAGGPGSGNNSSSGGASGAPSAVAAGGANAAVAGGAGGGGGAGAATAAATATGAIGATNAGGANVTDS | ||||||
Modified residue | 213 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 214 | Phosphotyrosine | ||||
Sequence: Y | ||||||
Modified residue | 217 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in ovaries and activated eggs (at protein level). Expression is over all the embryo at all stages, no local accumulation is observed.
Developmental stage
Isoform SGG46 is expressed at low levels in 12-24 hours embryos. Isoform Zygotic and isoform SGG39 are expressed in 12-24 hours embryos and present throughout the larval, pupal and adult stages (at protein level). Isoform Zygotic is expressed maternally and zygotically but reduced throughout later embryonic development. Expression persists throughout larval stages.
Gene expression databases
Interaction
Subunit
Interacts with cos.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P18431 | cry O77059 | 2 | EBI-242141, EBI-94117 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 54-338 | Protein kinase | ||||
Sequence: YTDTKVIGNGSFGVVFQAKLCDTGELVAIKKVLQDRRFKNRELQIMRKLEHCNIVKLLYFFYSSGEKRDEVFLNLVLEYIPETVYKVARQYAKTKQTIPINFIRLYMYQLFRSLAYIHSLGICHRDIKPQNLLLDPETAVLKLCDFGSAKQLLHGEPNVSYICSRYYRAPELIFGAINYTTKIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEVIKVLGTPTREQIREMNPNYTEFKFPQIKSHPWQKVFRIRTPTEAINLVSLLLEYTPSARITPLKACAHPFF | ||||||
Region | 379-470 | Disordered | ||||
Sequence: KHLQNASGPGGNRPSAGGAASIAASGSTSVSSTGSGASVEGSAQPQSQGTAAAAGSGSGGATAGTGGASAGGPGSGNNSSSGGASGAPSAVA | ||||||
Compositional bias | 399-433 | Polar residues | ||||
Sequence: SIAASGSTSVSSTGSGASVEGSAQPQSQGTAAAAG | ||||||
Compositional bias | 449-464 | Polar residues | ||||
Sequence: GGPGSGNNSSSGGASG |
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing. Additional isoforms seem to exist.
P18431-2
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameZygotic
- SynonymsB, C
- NoteMajor isoform.
- Length514
- Mass (Da)53,872
- Last updated2012-09-05 v3
- Checksum193EE2A1294BE494
P18431-1
- NameSGG46
- SynonymsMaternal, D
- Differences from canonical
- 1-29: MSGRPRTSSFAEGNKQSPSLVLGGVKTCS → MATTTTTQRAGAAPALNLLPASNNNINNTLINNNNNNNNTSNSNNNNNNVISQPIKIPLTERFSSQTSTGSADSGVIVSSASQQQLQLPPPRSSSGSLSLPQAPPGGKWRQKQQRQQLLLSQDSGIENGVTTRPSKAKDNQGAGKASHNATSSKESGAQSNSSSESLGSNCSEAQEQQRVRASSALELSSVDTPVIVGGVVSGGNSILRSRIKYKSTNSTGTQGFDVEDRIDEVDICDDDDVDCDDRGSEIEEEEEEEEDDGVNVDDDVEEADNQSDNQSGIIINLKSQTEQEEEVDEVDAKPKNRLLPPDQAELTVAAAMARRRDAKSLATDGHIYFPLLKISEDPHIDSKLINRKDGLQDTMYYLDEFGSPKLREKFARKQKQLLAKQQKQLMKRERRSEEQRKKRNTTVASNLAASGAVVDDTKDDYKQQPHCDTSSRSKNNSVPNPPSSHLHQNHNHLVVDVQEDVDDVNVVATSDVDSGVVKMRRHSHDNHYDRIPRSNAATITTRPQIDQQSSHHQNTEDVEQGAEPQIDGEADLDADADADSDGSGENVKTAKLARTQSCVSWTKVVQKFKNILG
P18431-3
- NameSGG39
- SynonymsA, J
- Differences from canonical
- 513-514: DS → GSQSNSALNSSGSGGSGNGEAAGSGSGSGSGSGGGNGGDNDAGDSGAIASGGGAAETEAAASG
P18431-4
- NameG
- NoteMajor isoform.
- Differences from canonical
- 1-29: MSGRPRTSSFAEGNKQSPSLVLGGVKTCS → MLINRGSLLEG
Computationally mapped potential isoform sequences
There are 8 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for alternative sequence, sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_044104 | 1-29 | in isoform G | |||
Sequence: MSGRPRTSSFAEGNKQSPSLVLGGVKTCS → MLINRGSLLEG | ||||||
Alternative sequence | VSP_044105 | 1-29 | in isoform SGG46 | |||
Sequence: MSGRPRTSSFAEGNKQSPSLVLGGVKTCS → MATTTTTQRAGAAPALNLLPASNNNINNTLINNNNNNNNTSNSNNNNNNVISQPIKIPLTERFSSQTSTGSADSGVIVSSASQQQLQLPPPRSSSGSLSLPQAPPGGKWRQKQQRQQLLLSQDSGIENGVTTRPSKAKDNQGAGKASHNATSSKESGAQSNSSSESLGSNCSEAQEQQRVRASSALELSSVDTPVIVGGVVSGGNSILRSRIKYKSTNSTGTQGFDVEDRIDEVDICDDDDVDCDDRGSEIEEEEEEEEDDGVNVDDDVEEADNQSDNQSGIIINLKSQTEQEEEVDEVDAKPKNRLLPPDQAELTVAAAMARRRDAKSLATDGHIYFPLLKISEDPHIDSKLINRKDGLQDTMYYLDEFGSPKLREKFARKQKQLLAKQQKQLMKRERRSEEQRKKRNTTVASNLAASGAVVDDTKDDYKQQPHCDTSSRSKNNSVPNPPSSHLHQNHNHLVVDVQEDVDDVNVVATSDVDSGVVKMRRHSHDNHYDRIPRSNAATITTRPQIDQQSSHHQNTEDVEQGAEPQIDGEADLDADADADSDGSGENVKTAKLARTQSCVSWTKVVQKFKNILG | ||||||
Sequence conflict | 9 | In isoform P18431-1; in Ref. 3; AAF45801 and 5; CAA19676/CAB65860 | ||||
Sequence: R → A | ||||||
Sequence conflict | 244 | in Ref. 2; CAA37419 | ||||
Sequence: V → I | ||||||
Sequence conflict | 256-259 | In isoform P18431-1; in Ref. 7; CAA37952 | ||||
Sequence: EEEE → E | ||||||
Sequence conflict | 258-259 | In isoform P18431-1; in Ref. 5; CAA19676/CAB65860 | ||||
Sequence: EE → E | ||||||
Sequence conflict | 397 | in Ref. 7; CAA37952 | ||||
Sequence: A → R | ||||||
Compositional bias | 399-433 | Polar residues | ||||
Sequence: SIAASGSTSVSSTGSGASVEGSAQPQSQGTAAAAG | ||||||
Sequence conflict | 406 | in Ref. 2; CAA37419 and 7; AAM50318 | ||||
Sequence: T → A | ||||||
Sequence conflict | 446 | in Ref. 7; CAA37952 | ||||
Sequence: A → R | ||||||
Compositional bias | 449-464 | Polar residues | ||||
Sequence: GGPGSGNNSSSGGASG | ||||||
Sequence conflict | 462 | in Ref. 7; CAA37952 | ||||
Sequence: A → R | ||||||
Sequence conflict | 473 | in Ref. 7; CAA37952 | ||||
Sequence: Missing | ||||||
Alternative sequence | VSP_044106 | 513-514 | in isoform SGG39 | |||
Sequence: DS → GSQSNSALNSSGSGGSGNGEAAGSGSGSGSGSGGGNGGDNDAGDSGAIASGGGAAETEAAASG | ||||||
Sequence conflict | 513-514 | in Ref. 5; CAA19676/CAB65860 | ||||
Sequence: DS → GE | ||||||
Sequence conflict | 539-540 | In isoform P18431-1; in Ref. 7; CAA37952 | ||||
Sequence: AD → RI |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X70862 EMBL· GenBank· DDBJ | CAA50212.1 EMBL· GenBank· DDBJ | mRNA | ||
X70863 EMBL· GenBank· DDBJ | CAA50213.1 EMBL· GenBank· DDBJ | mRNA | ||
X70864 EMBL· GenBank· DDBJ | CAA50214.1 EMBL· GenBank· DDBJ | mRNA | ||
X70865 EMBL· GenBank· DDBJ | CAA50215.1 EMBL· GenBank· DDBJ | mRNA | ||
X70866 EMBL· GenBank· DDBJ | CAA50216.1 EMBL· GenBank· DDBJ | mRNA | Sequence problems. | |
X53332 EMBL· GenBank· DDBJ | CAA37419.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014298 EMBL· GenBank· DDBJ | AAF45801.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | AAN09082.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | AAN09083.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | AAS65255.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL024485 EMBL· GenBank· DDBJ | CAA19676.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL034544 EMBL· GenBank· DDBJ | CAA19676.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL121804 EMBL· GenBank· DDBJ | CAB65860.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL024485 EMBL· GenBank· DDBJ | CAB65860.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL121804 EMBL· GenBank· DDBJ | CAB72296.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL024485 EMBL· GenBank· DDBJ | CAB72296.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY122193 EMBL· GenBank· DDBJ | AAM52705.1 EMBL· GenBank· DDBJ | mRNA | ||
AY119664 EMBL· GenBank· DDBJ | AAM50318.1 EMBL· GenBank· DDBJ | mRNA | ||
X54005 EMBL· GenBank· DDBJ | CAA37951.1 EMBL· GenBank· DDBJ | mRNA | ||
X54006 EMBL· GenBank· DDBJ | CAA37952.1 EMBL· GenBank· DDBJ | mRNA | Frameshift |