P18238 · ADT3_YEAST
- ProteinADP,ATP carrier protein 3
- GeneAAC3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids307 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (PubMed:2165073).
Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity).
Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity).
Miscellaneous
Present with 1080 molecules/cell in log phase SD medium.
Catalytic activity
- ADP(in) + ATP(out) = ADP(out) + ATP(in)This reaction proceeds in the forward direction.
Activity regulation
The matrix-open state (m-state) is inhibited by the membrane-permeable bongkrekic acid (BKA). The cytoplasmic-open state (c-state) is inhibited by the membrane-impermeable toxic inhibitor carboxyatractyloside (CATR).
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Cellular Component | mitochondrion | |
Molecular Function | ATP:ADP antiporter activity | |
Molecular Function | identical protein binding | |
Biological Process | anaerobic respiration | |
Biological Process | heme transport | |
Biological Process | mitochondrial ADP transmembrane transport | |
Biological Process | mitochondrial ATP transmembrane transport | |
Biological Process | transmembrane transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameADP,ATP carrier protein 3
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP18238
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 12-39 | Helical; Name=1 | ||||
Sequence: FAINFLMGGVSAAIAKTAASPIERVKIL | ||||||
Transmembrane | 80-104 | Helical; Name=2 | ||||
Sequence: TANVIRYFPTQALNFAFKDKIKLMF | ||||||
Transmembrane | 112-132 | Helical; Name=3 | ||||
Sequence: YGKWFAGNLASGGAAGALSLL | ||||||
Transmembrane | 182-203 | Helical; Name=4 | ||||
Sequence: FMPSVVGIVVYRGLYFGMFDSL | ||||||
Transmembrane | 217-237 | Helical; Name=5 | ||||
Sequence: LASFLLGWVVTTGASTCSYPL | ||||||
Transmembrane | 277-297 | Helical; Name=6 | ||||
Sequence: CGANILRSVAGAGVISMYDQL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000090595 | 1-307 | ADP,ATP carrier protein 3 | |||
Sequence: MSSDAKQQETNFAINFLMGGVSAAIAKTAASPIERVKILIQNQDEMIKQGTLDKKYSGIVDCFKRTAKQEGLISFWRGNTANVIRYFPTQALNFAFKDKIKLMFGFKKEEGYGKWFAGNLASGGAAGALSLLFVYSLDFARTRLAADAKSSKKGGARQFNGLTDVYKKTLKSDGIAGLYRGFMPSVVGIVVYRGLYFGMFDSLKPLVLTGSLDGSFLASFLLGWVVTTGASTCSYPLDTVRRRMMMTSGQAVKYNGAIDCLKKIVASEGVGSLFKGCGANILRSVAGAGVISMYDQLQMILFGKKFK |
Proteomic databases
PTM databases
Interaction
Subunit
Monomer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P18238 | AAC3 P18238 | 2 | EBI-2300, EBI-2300 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for repeat, region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 10-103 | Solcar 1 | ||||
Sequence: TNFAINFLMGGVSAAIAKTAASPIERVKILIQNQDEMIKQGTLDKKYSGIVDCFKRTAKQEGLISFWRGNTANVIRYFPTQALNFAFKDKIKLM | ||||||
Repeat | 114-206 | Solcar 2 | ||||
Sequence: KWFAGNLASGGAAGALSLLFVYSLDFARTRLAADAKSSKKGGARQFNGLTDVYKKTLKSDGIAGLYRGFMPSVVGIVVYRGLYFGMFDSLKPL | ||||||
Repeat | 214-300 | Solcar 3 | ||||
Sequence: GSFLASFLLGWVVTTGASTCSYPLDTVRRRMMMTSGQAVKYNGAIDCLKKIVASEGVGSLFKGCGANILRSVAGAGVISMYDQLQMI | ||||||
Region | 241-246 | Important for transport activity | ||||
Sequence: RRRMMM | ||||||
Motif | 241-246 | Nucleotide carrier signature motif | ||||
Sequence: RRRMMM |
Domain
The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue.
Sequence similarities
Belongs to the mitochondrial carrier (TC 2.A.29) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length307
- Mass (Da)33,313
- Last updated1990-11-01 v1
- ChecksumD0C1329FEC1B4DC8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M34076 EMBL· GenBank· DDBJ | AAA97485.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z35954 EMBL· GenBank· DDBJ | CAA85031.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07205.1 EMBL· GenBank· DDBJ | Genomic DNA |