P18099 · VPX_HV2BE

Function

function

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
Targets specific host proteins for degradation by the 26S proteasome (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1 (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1 (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).

Miscellaneous

This isolate is from a German AIDS patient (with predominantly neurological complications) who was probably infected in Mali.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componenthost cell nucleus
Cellular Componentvirion component
Biological Processsymbiont-mediated suppression of host innate immune response
Biological Processviral life cycle
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein Vpx
  • Alternative names
    • Viral protein X
    • X ORF protein

Gene names

    • Name
      vpx

Organism names

Accessions

  • Primary accession
    P18099

Proteomes

Subcellular Location

Note: Nuclear just after virion uncoating, or if expressed in the absence of unprocessed GAG.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis76Complete loss of interaction with host DCAF1.

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000853901-113Protein Vpx

Interaction

Subunit

Interacts with the P6 region of unprocessed GAG (By similarity).
Interacts with host VPRBP/DCAF1, leading to change substrate specificity of the CUL4A-DDB1 E3 ligase complex (PubMed:19264781).
Interacts with host NUP153 (By similarity).

Protein-protein interaction databases

Structure

3D structure databases

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region61-80Binds to human NUP153
Motif65-72Nuclear localization signal
Region94-113Disordered

Sequence similarities

Belongs to the lentivirus VPX protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    113
  • Mass (Da)
    13,208
  • Last updated
    1990-11-01 v1
  • Checksum
    2C18958819216B24
MTDPRERVPPGNSGEETIGEAFEWLERTIEALNREAVNHLPRELIFQVWQRSWRYWHDEQGMSASYTKYRYLCLMQKAIFTHFKRGCTCWGEDMGREGLEDQGPPPPPPPGLV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M30502
EMBL· GenBank· DDBJ
AAB00739.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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