P18099 · VPX_HV2BE
- ProteinProtein Vpx
- Genevpx
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids113 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
Targets specific host proteins for degradation by the 26S proteasome (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1 (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1 (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
Targets specific host proteins for degradation by the 26S proteasome (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1 (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1 (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (PubMed:19264781, PubMed:21613998, PubMed:21720370, PubMed:22973040).
Miscellaneous
This isolate is from a German AIDS patient (with predominantly neurological complications) who was probably infected in Mali.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Cellular Component | virion component | |
Biological Process | symbiont-mediated suppression of host innate immune response | |
Biological Process | viral life cycle | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein Vpx
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Riboviria > Pararnavirae > Artverviricota > Revtraviricetes > Ortervirales > Retroviridae > Orthoretrovirinae > Lentivirus > Human immunodeficiency virus 2
- Virus hosts
Accessions
- Primary accessionP18099
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Nuclear just after virion uncoating, or if expressed in the absence of unprocessed GAG.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 76 | Complete loss of interaction with host DCAF1. | ||||
Sequence: Q → LYS |
Keywords
- Disease
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000085390 | 1-113 | Protein Vpx | |||
Sequence: MTDPRERVPPGNSGEETIGEAFEWLERTIEALNREAVNHLPRELIFQVWQRSWRYWHDEQGMSASYTKYRYLCLMQKAIFTHFKRGCTCWGEDMGREGLEDQGPPPPPPPGLV |
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 61-80 | Binds to human NUP153 | ||||
Sequence: GMSASYTKYRYLCLMQKAIF | ||||||
Motif | 65-72 | Nuclear localization signal | ||||
Sequence: SYTKYRYL | ||||||
Region | 94-113 | Disordered | ||||
Sequence: MGREGLEDQGPPPPPPPGLV |
Sequence similarities
Belongs to the lentivirus VPX protein family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length113
- Mass (Da)13,208
- Last updated1990-11-01 v1
- Checksum2C18958819216B24
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M30502 EMBL· GenBank· DDBJ | AAB00739.1 EMBL· GenBank· DDBJ | Genomic DNA |