P17595 · ALPHA_BSMV

Function

function

Probably contains methyltransferase and helicase activities (PubMed:2209552).
Methyltransferase displays a cytoplasmic capping enzyme activity (Probable). This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus (Probable). Helicase region probably exhibits NTPase and RNA unwinding activities (Probable)

Miscellaneous

The genome of this virus consists of three linear, positive, single-stranded RNAs encapsidated in separate virions designated RNA-alpha, RNA-beta and RNA-gamma. Three proteins (alpha-A, beta-A and gamma-A) are translated directly from these genomic RNAs and the remaining proteins encoded on RNA-beta (beta-B, beta-C and beta-D) and RNA-gamma (gamma-B) are expressed via three subgenomic messenger RNAs.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site838-845ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionmRNA methyltransferase activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Biological Processmethylation
Biological ProcessmRNA modification
Biological ProcessRNA processing

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Replication protein alpha-A

Including 2 domains:

Organism names

Accessions

  • Primary accession
    P17595

Proteomes

Subcellular Location

Note: The viral replication sites are located at the host chloroplast membrane.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002224921-1139Replication protein alpha-A

Interaction

Subunit

Interacts with the suppressor of RNA silencing Gamma-B (via C-terminus).

Structure

3D structure databases

Family & Domains

Features

Showing features for region, domain, compositional bias.

Type
IDPosition(s)Description
Region60-438Methyltransferase
Domain81-305Alphavirus-like MT
Region611-637Disordered
Compositional bias614-637Polar residues
Domain805-969+RNA virus helicase ATP-binding
Region835-1109Helicase
Domain970-1139+RNA virus helicase C-terminal

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,139
  • Mass (Da)
    129,628
  • Last updated
    1990-08-01 v1
  • Checksum
    24B1500FD05362D9
MASDEIVRNLISREEVMGNLISTASSSVRSPLHDVLCSHVRTIVDSVDKKAVSRKHEDVRRNISSEELQMLINAYPEYAVSSSACESGTHSMAACFRFLETEYLLDMVPMKETFVYDIGGNWFSHMKFRADREIHCCCPILSMRDSERLETRMMAMQKYMRGSKDKPLRLLSRYQNILREQAARTTAFMAGEVNAGVLDGDVFCENTFQDCVRRVPEGFLKTAIAVHSIYDIKVEEFASALKRKGITQAYGCFLFPPAVLIGQKEGILPSVDGHYLVENGRIKFFFANDPNAGYSHDLKDYLKYVEKTYVDIEDGVFAIELMQMRGDTMFFKITDVTAAMYHMKYRGMKRDETFKCIPLLKNSSVVVPLFSWDNRSLKITSGLLPRTLVEQGAAFIMKNKEKDLNVAVLKNYLSAVNNSYIFNGSQVRDGVKIAPDLISKLAVTLYLREKVYRQRENSIISYFEQEMLHDPNLKAMFGDFLWFVPNTLSSVWKNMRKSLMEWFGYAEFDLTTFDICDPVLYVEIVDRYKIIQKGRIPLGEFFDCHEECENYELREKEKNDLAVKMAQKVTGTVTECEKDLGPLVQPIKEILVQLVMPNLVRALCRPRSPTSPLDLKSIPGSTPSHSSSDSEHSMTEEASCTIAGSVPTWEIATRKDLTFQRIDEDMSRRTGMPPRPKVTSSYNMNARAEFLYYQLCSVICERAQILSVIEDFRQNLIFSDKVAVPLNARFYSFQSLRPGWVFKTPSHSEVGHSYAVHFDFKTIGTDLEESLAFCRMVPISWDKSGKYIATTPHFPERHGYYVICDNTKLCNNWLIYNKLVDVYALVADRPLRFELIDGVPGCGKSTMILNSCDIRREVVVGEGRNATDDLRERFKRKKNLNSKTANHRVRTLDSLLLAEGPCVPQADRFHFDEALKVHYGAIMFCADKLGASEILAQGDRAQLPMICRVEGIELQFQSPDYTKTIINPKLRSYRIPGDVAFYLSAKEFYKVKGIPQKVITSNSVKRSLYARGETTPERFVSLLDVPVRKDTHYLTFLQAEKESLMSHLIPKGVKKESISTIHEAQGGTYENVILVRLQRTPNEIYPGGPRSAPYIVVGTSRHTKTFTYCSVTDDKLLLDIADVGGIAHTPIRTFESHIV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias614-637Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
J04342
EMBL· GenBank· DDBJ
AAA46336.1
EMBL· GenBank· DDBJ
Genomic RNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help