P17410 · CHBR_ECOLI
- ProteinHTH-type transcriptional regulator ChbR
- GenechbR
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids280 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Dual-function repressor/activator of the chbBCARFG operon. In the absence of the inducing sugar chitobiose, together with NagC, represses the chbBCARFG operon for the uptake and metabolism of chitobiose. In association with Crp, and probably in the presence of chitobiose 6-phosphate, induces the transcription of the chbBCARFG operon.
Miscellaneous
Under prolonged growth on cellobiose as the sole carbon source, spontaneous mutations in chbR and nagC alter the regulation of the chbBCARFG operon, allowing the uptake and metabolism of cellobiose. The mutated regulator makes stronger contact with the target DNA sequence within the chb promoter and has enhanced recognition of cellobiose 6-phosphate as an inducer.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 193-214 | H-T-H motif | ||||
Sequence: ENMVALSAKSQEYLTRATQRYY | ||||||
DNA binding | 241-264 | H-T-H motif | ||||
Sequence: VTDIAFEAGYSSPSLFIKTFKKLT |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | core promoter sequence-specific DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | protein homodimerization activity | |
Biological Process | negative regulation of DNA-templated transcription |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHTH-type transcriptional regulator ChbR
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP17410
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000194506 | 1-280 | HTH-type transcriptional regulator ChbR | |||
Sequence: MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYYEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHYREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVALSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTEFNQ |
Proteomic databases
Expression
Induction
By N,N'-diacetylchitobiose.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 29-81 | Cupin type-2 | ||||
Sequence: IYNKTESISGLHQHDYYEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQ | ||||||
Domain | 168-274 | HTH araC/xylS-type | ||||
Sequence: DDVPQWLKSTVEKMHDKEQFSESALENMVALSAKSQEYLTRATQRYYGKTPMQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKK |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length280
- Mass (Da)32,968
- Last updated1990-08-01 v1
- Checksum281152CAEF363B2F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X52890 EMBL· GenBank· DDBJ | CAA37072.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC74805.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA15516.1 EMBL· GenBank· DDBJ | Genomic DNA |