P16716 · RP132_SHEVK
- ProteinDNA-directed RNA polymerase 132 kDa polypeptide
- GeneRPO132
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids919 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of D6 and A7, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase (By similarity).
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | virion component | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | ribonucleoside binding | |
Molecular Function | RNA polymerase I activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase 132 kDa polypeptide
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Nucleocytoviricota > Pokkesviricetes > Chitovirales > Poxviridae > Chordopoxvirinae > Capripoxvirus > Sheeppox virus
- Virus hosts
Accessions
- Primary accessionP16716
Subcellular Location
UniProt Annotation
GO Annotation
Note: All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome. This is necessary because viral early mRNAs are synthesized within minutes after virus entry into the cell and are extruded through pores in the core particle (By similarity).
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000048065 | 1-919 | DNA-directed RNA polymerase 132 kDa polypeptide | |||
Sequence: SFIKHSLSYDMPSEISYLVNTIIESTKELIKTINDFDIDTYINDLIISEYNKQKSQLILEEFKHEMINNFLPHMNDTPNQLKGFYIMSLLRKFIYCIYYTSRYPDRDSMVCHRVLTYGKYFEILAHDELENYIGNIRTDIINNHKNRGTYSVNIHVLTTPGFNHAFSGLLSGKFKKTDGSYRTHSHYSWMQNISIPRSVGYYPDQVKISKMFSVRKYHPSQYAYFCPSDVPERGPQVGLVSQLSVLTSITNICTNEYLELEKKICNYIRSYNHNDISYFETGYYITLENSLIACLNPNLVDDFVIDFRRKKRMNYFGNLEIGITLVNDHMNEIRINIGGGRLIRPFLVIDNGNLIMDEIFSELEFKIDDMTFSDIQKEFPHVIEIVDIEQFTFSNVCESVQKFRALPKSEKCKYHLCDFPAEFKDGYVASSLVGINHNSGPRAILGCAQRKQAISCLSSDIRNKIDNGIHLIYPERPIVISKALETSKIAVNCFGQHVTIALMSYKGINQEDGIIIKKQFVERGGLDIITAKKHQVEIPLENFNNKERVKSTAYSKLESNGLVRLNAFLESGDAIARNISSRTLEDDFVQDNQISFDISDRYTDMYQSRVERVQVDLTDKVKVRVLTMKERRPVLGDKFTSRTSQKGTVAYIADETELPYDENGIKPDVIINSTSIFSRKTVSMLIEVILTSAYEVSPYNNDGQNRPICFPSSNETSIDTYLDFAKRCHRDRYPSLSDDDINDKMFCDTILYDPETDKPYSSKIFMGPIYYLRLRHLTQDKATVRCRGKKTKLIRQANEGRRRGGGIKFGEMERDCLIAHGAANTITEVLKDSEEDYQDVYVCENCGDITAQIQGNKVCIRCSKQNLSTILTKVDTTHVAKVFITQMNARGVKVKLEFEKRNPLFYKPLDVVDLSPNFL |
Interaction
Subunit
The DNA-dependent RNA polymerase used for intermediate and late genes expression consists of eight subunits 147 kDa, 133 kDa, 35 kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500 kDa in mass. The same holoenzyme, with the addition of the transcription-specificity factor RAP94, is used for early gene expression (By similarity).
Sequence
- Sequence statusFragment
- Length919
- Mass (Da)105,702
- Last updated1990-08-01 v1
- Checksum5395E8E1008AB6D6
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: S |