P16461 · LTXC_AGGAC
- ProteinLeukotoxin-activating lysine-acyltransferase LtxC
- GeneltxC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids168 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Required for full activity and modification of the LtxA leukotoxin. Involved in fatty acid modification of the protoxin at two internal lysine residues, thereby converting it to the active toxin.
Catalytic activity
- a fatty acyl-[ACP] + L-lysyl-[protein] = N6-(fatty acyl)-L-lysyl-[protein] + holo-[ACP] + H+This reaction proceeds in the forward direction.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 23 | |||||
Sequence: H | ||||||
Active site | 92 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | acyltransferase activity | |
Biological Process | toxin metabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLeukotoxin-activating lysine-acyltransferase LtxC
- EC number
- Short namesLeukotoxin C; Toxin-activating protein C
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pasteurellales > Pasteurellaceae > Aggregatibacter
Accessions
- Primary accessionP16461
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Mutants can express and secrete the LtxA leukotoxin, but LtxA lacks both leukotoxic and erythrolytic activities, and is unable to cause a change in intracellular calcium levels in host cells.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000217878 | 1-168 | Leukotoxin-activating lysine-acyltransferase LtxC | |||
Sequence: MEKNNNFEMLGYVAWLWANSPLHRNWSLSLLAINVLPAIQYGQYTLLMRDGVPIAFCSWANLSLENEIKYLEDVSSLVYDDWNSGDRKWFIDWIAPFGHNYVLYKHMRKSFPYDLFRSIRVYKGSSEGKITEFHGGKVDKQLANKIFQQYHFELINELKNKSEVISIN |
Expression
Induction
Levels of toxin expression vary greatly among strains. Highly leukotoxic strains (JP2-type strains) produce more LtxA protein and ltx mRNA than minimally leukotoxic strains (652-type strains). Variations are probably due to different types of promoters.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length168
- Mass (Da)19,793
- Last updated1990-08-01 v1
- Checksum9602C0369FCAF332
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 9 | in Ref. 2; CAA34730 | ||||
Sequence: M → V |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M27399 EMBL· GenBank· DDBJ | AAA21921.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X16829 EMBL· GenBank· DDBJ | CAA34730.1 EMBL· GenBank· DDBJ | Genomic DNA |