P16243 · MAOC_MAIZE

  • Protein
    NADP-dependent malic enzyme, chloroplastic
  • Gene
    MOD1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO2 released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO2 that occurs in most plants.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Divalent metal cations. Prefers magnesium or manganese.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.23 mMmalate
14.9 μMNADP
kcat is 28.1 sec-1 with malate as substrate.

pH Dependence

Optimum pH is 7.5-8.0.

Pathway

Photosynthesis; C4 acid pathway.

Features

Showing features for active site, binding site, site.

TypeIDPosition(s)Description
Active site184Proton donor
Binding site237NAD+ (UniProtKB | ChEBI)
Active site255Proton acceptor
Binding site327a divalent metal cation (UniProtKB | ChEBI)
Binding site328a divalent metal cation (UniProtKB | ChEBI)
Binding site351a divalent metal cation (UniProtKB | ChEBI)
Binding site351NAD+ (UniProtKB | ChEBI)
Site351Important for activity
Binding site380-396NADP+ (UniProtKB | ChEBI)
Binding site492NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Functionmalate dehydrogenase (decarboxylating) (NADP+) activity
Molecular Functionmetal ion binding
Molecular FunctionNAD binding
Molecular Functionoxaloacetate decarboxylase activity
Biological Processmalate metabolic process
Biological Processpyruvate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADP-dependent malic enzyme, chloroplastic
  • EC number
  • Short names
    NADP-ME

Gene names

    • Name
      MOD1
    • Synonyms
      ME1

Organism names

  • Taxonomic identifier
  • Strain
    • cv. B73 Inbred
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > PACMAD clade > Panicoideae > Andropogonodae > Andropogoneae > Tripsacinae > Zea

Accessions

  • Primary accession
    P16243

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis140Decreases kcat 8-fold. Decreases Km for NADP 2-fold, increases Km for malate 2-fold.
Mutagenesis237Decreases kcat 530-fold. Increases Km for NADP 36-fold and Km for malate 10-fold.
Mutagenesis255Increases Km for malate 10-fold, and Km for NADP 15-fold. Decreases kcat 200-fold.
Mutagenesis339No effect on kcat and Km for NADP. Increases Km for malate 2-fold.
Mutagenesis387Decreases kcat 48-fold. Increases Km for NADP 4-fold. Increases Km for malate 6-fold.
Mutagenesis392No effect on kcat. Increases Km for NADP 3.5-fold. Increases Km for malate 2.5-fold.
Mutagenesis435-436No effect on kcat and on Km for malate. Increases Km for NADP 9-fold.
Mutagenesis503No effect on kcat and Km for NADP. Increases Km for malate 2-fold.
Mutagenesis544No effect on kcat and Km for NADP. Increases Km for malate 2-fold.

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-62Chloroplast
ChainPRO_000001854763-636NADP-dependent malic enzyme, chloroplastic

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-28Disordered

Sequence similarities

Belongs to the malic enzymes family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    636
  • Mass (Da)
    69,824
  • Last updated
    1990-08-01 v1
  • Checksum
    DF2D36FD4B2682EA
MLSTRTAAVAASASPASPWKLGGRSEGGASCDGCRTYRNTLRRRAAPAKVRALPPRRVDAVAMVSNAETETEKEQEEAAAASEELPVMPWATSVASGYTLLRDPHHNKGLAFTEEERDGHYLRGLLPPAVLSQELQIKKFMNTLRQYQTPLQRYIAMMNLQETDERLFYKLLIDNVVELLPFVYTPTVGEACQKYGSIFGRPQGLYVSLKDKGKVLEVLRNWPHRNIQVICVTDGERILGLGDLGCQGMGIPVGKLALYTALGGVDPSVCLPITIDVGTNNEFLLNDEFYIGLRQKRATGEEYDELIEEFMSAVKQFYGEKVLIQFEDFANHNAFDLLEKYSKSHLVFNDDIQGTASVVLAGLLAALKMVGGTLAEQTYLFLGAGEAGTGIAELIALEISKQTNAPIEECRKKVWLVDSKGLIVDSRKGSLQPFKKPWAHEHEPLKTLYDAVQSIKPTVLIGTSGVGRTFTKEIIEAMSSFNERPIIFSLSNPTSHSECTAEQAYTWSQGRSIFASGSPFAPVEYEGKTFVPGQSNNAYIFPGLGLGLVISGAVRVHEDMLLAASKALADQATQDNFEKGSIFPPFTSIRKISAHIAAAVAGKAYELGLATRLPPPSDLVKYAENCMYTPVYRNYR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
J05130
EMBL· GenBank· DDBJ
AAA33487.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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