P16110 · LEG3_MOUSE

  • Protein
    Galectin-3
  • Gene
    Lgals3
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration (PubMed:15181153).
Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes (By similarity).
When secreted, interacts with NK cell-activating receptor NCR3/NKp30 acting as an inhibitory ligand which antagonizes NK cell attack (By similarity).

Features

Showing features for binding site.

126420406080100120140160180200220240260
TypeIDPosition(s)Description
Binding site195-201a beta-D-galactoside (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell surface
Cellular Componentcollagen-containing extracellular matrix
Cellular Componentcornified envelope
Cellular Componentcytoplasm
Cellular Componentexternal side of plasma membrane
Cellular Componentextracellular matrix
Cellular Componentextracellular region
Cellular Componentextracellular space
Cellular Componentglial cell projection
Cellular Componentimmunological synapse
Cellular Componentnucleus
Cellular Componentspliceosomal complex
Molecular Functionadvanced glycation end-product receptor activity
Molecular Functiondisaccharide binding
Molecular FunctionFc-gamma receptor I complex binding
Molecular FunctionIgE binding
Molecular Functionlaminin binding
Molecular Functionmonosaccharide binding
Biological Processcell differentiation
Biological Processeosinophil chemotaxis
Biological Processextracellular matrix organization
Biological Processinnate immune response
Biological Processmacrophage chemotaxis
Biological Processmonocyte chemotaxis
Biological ProcessmRNA processing
Biological Processnegative regulation of apoptotic process
Biological Processnegative regulation of cell proliferation in bone marrow
Biological Processnegative regulation of endocytosis
Biological Processnegative regulation of extrinsic apoptotic signaling pathway
Biological Processnegative regulation of immunological synapse formation
Biological Processnegative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
Biological Processnegative regulation of T cell receptor signaling pathway
Biological Processneutrophil chemotaxis
Biological Processpositive chemotaxis
Biological Processpositive regulation of angiogenesis
Biological Processpositive regulation of calcium ion import
Biological Processpositive regulation of cell population proliferation
Biological Processpositive regulation of serotonin secretion
Biological ProcessRNA splicing
Biological Processskeletal system development

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Galectin-3
  • Short names
    Gal-3
  • Alternative names
    • 35 kDa lectin
    • Carbohydrate-binding protein 35 (CBP 35)
    • Galactose-specific lectin 3
    • IgE-binding protein
    • L-34 galactoside-binding lectin

Gene names

    • Name
      Lgals3

Organism names

  • Taxonomic identifier
  • Strains
    • DBA/2J
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P16110

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Secreted
Note: Secreted by a non-classical secretory pathway and associates with the cell surface. Can be secreted; the secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10; it results in protein translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) followed by vesicle entry and secretion.

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain, disulfide bond.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00000769312-264Galectin-3
Modified residue6Phosphoserine; by CK1
Disulfide bond187Interchain
Modified residue202Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

The highest levels are found in activated macrophages.

Interaction

Subunit

Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity).
Interacts with CD6 and ALCAM. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, ZFTRAF1 and UACA. Interacts with TRIM16; this interaction mediates autophagy of damage endomembranes (By similarity).
Interacts with and inhibits by binding NCR3/NKp30 (By similarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P16110Chi3l1 Q613624EBI-3508325, EBI-8392424
BINARY P16110Il13ra2 O887862EBI-3508325, EBI-20260800
BINARY P16110Sufu Q9Z0P75EBI-3508325, EBI-3508336

Protein-protein interaction databases

Chemistry

Miscellaneous

Family & Domains

Features

Showing features for compositional bias, region, repeat, domain, motif.

TypeIDPosition(s)Description
Compositional bias1-15Polar residues
Region1-105Disordered
Repeat35-431
Region35-1149 X 9 AA tandem repeats of Y-P-G-X3-P-[GS]-A
Repeat44-522
Compositional bias47-102Pro residues
Repeat53-613
Repeat62-704
Repeat71-795
Repeat80-886
Repeat89-977
Repeat98-1078
Repeat108-1149; truncated
Domain132-262Galectin
Motif240-255Nuclear export signal

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    264
  • Mass (Da)
    27,515
  • Last updated
    2007-01-23 v3
  • Checksum
    1B5A8A81093D68F6
MADSFSLNDALAGSGNPNPQGYPGAWGNQPGAGGYPGAAYPGAYPGQAPPGAYPGQAPPGAYPGQAPPSAYPGPTAPGAYPGPTAPGAYPGQPAPGAFPGQPGAPGAYPQCSGGYPAAGPYGVPAGPLTVPYDLPLPGGVMPRMLITIMGTVKPNANRIVLDFRRGNDVAFHFNPRFNENNRRVIVCNTKQDNNWGKEERQSAFPFESGKPFKIQVLVEADHFKVAVNDAHLLQYNHRMKNLREISQLGISGDITLTSANHAMI

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2I3BRE5A0A2I3BRE5_MOUSELgals332
Q8C253Q8C253_MOUSELgals3264
D3YZJ2D3YZJ2_MOUSELgals3139
D3YVT4D3YVT4_MOUSELgals394

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias1-15Polar residues
Sequence conflict2in Ref. 2; AAA37311
Sequence conflict4in Ref. 3; CAA34206
Compositional bias47-102Pro residues
Sequence conflict92-93in Ref. 3; CAA34206
Sequence conflict110-112in Ref. 3; CAA34206
Sequence conflict252in Ref. 2; AAA37311

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X16834
EMBL· GenBank· DDBJ
CAA34736.1
EMBL· GenBank· DDBJ
mRNA
J03723
EMBL· GenBank· DDBJ
AAA37311.1
EMBL· GenBank· DDBJ
mRNA
X16074
EMBL· GenBank· DDBJ
CAA34206.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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