P15807 · MET8_YEAST
- ProteinSiroheme biosynthesis protein MET8
- GeneMET8
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids274 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the conversion of precorrin-2 into siroheme. This reaction consist of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme.
Catalytic activity
- NAD+ + precorrin-2 = 2 H+ + NADH + sirohydrochlorinThis reaction proceeds in the forward direction.
Pathway
Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ferrochelatase activity | |
Molecular Function | precorrin-2 dehydrogenase activity | |
Molecular Function | sirohydrochlorin ferrochelatase activity | |
Biological Process | siroheme biosynthetic process | |
Biological Process | sulfate assimilation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSiroheme biosynthesis protein MET8
Including 2 domains:
- Recommended namePrecorrin-2 dehydrogenase
- EC number
- Recommended nameSirohydrochlorin ferrochelatase
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP15807
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 22 | Loss of dehydrogenase activity. No effect on chelatase activity. | ||||
Sequence: G → D | ||||||
Mutagenesis | 141 | Loss of chelatase and dehydrogenase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 237 | No effect on dehydrogenase or chelatase activity. | ||||
Sequence: H → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000096446 | 1-274 | Siroheme biosynthesis protein MET8 | |||
Sequence: MVKSLQLAHQLKDKKILLIGGGEVGLTRLYKLIPTGCKLTLVSPDLHKSIIPKFGKFIQNEDQPDYREDAKRFINPNWDPTKNEIYEYIRSDFKDEYLDLEDENDAWYIIMTCIPDHPESARIYHLCKERFGKQQLVNVADKPDLCDFYFGANLEIGDRLQILISTNGLSPRFGALVRDEIRNLFTQMGDLALEDAVVKLGELRRGIRLLAPDDKDVKYRMDWARRCTDLFGIQHCHNIDVKRLLDLFKVMFQEQNCSLQFPPRERLLSEYCSS |
Proteomic databases
PTM databases
Structure
Family & Domains
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length274
- Mass (Da)31,918
- Last updated1990-04-01 v1
- Checksum0EE9A6DE8A43673E
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 15 | in Ref. 5 | ||||
Sequence: K → R | ||||||
Sequence conflict | 33 | in Ref. 5 | ||||
Sequence: I → M | ||||||
Sequence conflict | 61 | in Ref. 5 | ||||
Sequence: E → K | ||||||
Sequence conflict | 102 | in Ref. 5 | ||||
Sequence: D → N |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X17271 EMBL· GenBank· DDBJ | CAA35173.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z36082 EMBL· GenBank· DDBJ | CAA85177.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07329.1 EMBL· GenBank· DDBJ | Genomic DNA |