P15731 · UBC4_YEAST

Function

function

Catalyzes the covalent attachment of ubiquitin to other proteins (PubMed:17550898).
Mediates the selective degradation of short-lived and abnormal proteins (PubMed:17550898).
Mediates ubiquitination of PEX5 (PubMed:17550898).

Miscellaneous

Present with 13500 molecules/cell in log phase SD medium.

Catalytic activity

  • S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.
    EC:2.3.2.23 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for active site.

114820406080100120140
TypeIDPosition(s)Description
Active site86Glycyl thioester intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular Componentubiquitin ligase complex
Molecular FunctionATP binding
Molecular Functionproteasome binding
Molecular Functionprotein-macromolecule adaptor activity
Molecular Functionubiquitin binding
Molecular Functionubiquitin conjugating enzyme activity
Molecular Functionubiquitin protein ligase binding
Molecular Functionubiquitin-protein transferase activity
Biological Processcellular response to heat
Biological Processcytoplasm protein quality control by the ubiquitin-proteasome system
Biological Processmitochondria-associated ubiquitin-dependent protein catabolic process
Biological Processprotein polyubiquitination
Biological Processrescue of stalled ribosome
Biological Processribosome-associated ubiquitin-dependent protein catabolic process
Biological Processubiquitin-dependent protein catabolic process
Biological Processubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin-conjugating enzyme E2 4
  • EC number
  • Alternative names
    • E2 ubiquitin-conjugating enzyme 4
    • Ubiquitin carrier protein 4
    • Ubiquitin-protein ligase 4

Gene names

    • Name
      UBC4
    • ORF names
      YBR0745
    • Ordered locus names
      YBR082C

Organism names

Accessions

  • Primary accession
    P15731
  • Secondary accessions
    • D6VQ81

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Defective activation of the ribosome quality control (RQC) pathway.

PTM/Processing

Features

Showing features for chain, modified residue, cross-link.

TypeIDPosition(s)Description
ChainPRO_00000825451-148Ubiquitin-conjugating enzyme E2 4
Modified residue12Phosphoserine
Cross-link91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Post-translational modification

The N-terminus is blocked.

Keywords

Proteomic databases

PTM databases

Expression

Induction

By heat shock and cadmium.

Interaction

Subunit

Interacts with TUL1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P15731EGD2 P388792EBI-19735, EBI-6379
BINARY P15731MOT2 P349093EBI-19735, EBI-12174

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-22Disordered
Domain2-148UBC core

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    148
  • Mass (Da)
    16,456
  • Last updated
    1990-04-01 v1
  • Checksum
    8E96137D3EB20F80
MSSSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATAREWTKKYAV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X17493
EMBL· GenBank· DDBJ
CAA35528.1
EMBL· GenBank· DDBJ
Genomic DNA
X76294
EMBL· GenBank· DDBJ
CAA53942.1
EMBL· GenBank· DDBJ
Genomic DNA
Z35951
EMBL· GenBank· DDBJ
CAA85027.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006936
EMBL· GenBank· DDBJ
DAA07201.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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