P15731 · UBC4_YEAST
- ProteinUbiquitin-conjugating enzyme E2 4
- GeneUBC4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids148 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the covalent attachment of ubiquitin to other proteins (PubMed:17550898).
Mediates the selective degradation of short-lived and abnormal proteins (PubMed:17550898).
Mediates ubiquitination of PEX5 (PubMed:17550898).
Mediates the selective degradation of short-lived and abnormal proteins (PubMed:17550898).
Mediates ubiquitination of PEX5 (PubMed:17550898).
Miscellaneous
Present with 13500 molecules/cell in log phase SD medium.
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 86 | Glycyl thioester intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Cellular Component | ubiquitin ligase complex | |
Molecular Function | ATP binding | |
Molecular Function | proteasome binding | |
Molecular Function | protein-macromolecule adaptor activity | |
Molecular Function | ubiquitin binding | |
Molecular Function | ubiquitin conjugating enzyme activity | |
Molecular Function | ubiquitin protein ligase binding | |
Molecular Function | ubiquitin-protein transferase activity | |
Biological Process | cellular response to heat | |
Biological Process | cytoplasm protein quality control by the ubiquitin-proteasome system | |
Biological Process | mitochondria-associated ubiquitin-dependent protein catabolic process | |
Biological Process | protein polyubiquitination | |
Biological Process | rescue of stalled ribosome | |
Biological Process | ribosome-associated ubiquitin-dependent protein catabolic process | |
Biological Process | ubiquitin-dependent protein catabolic process | |
Biological Process | ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUbiquitin-conjugating enzyme E2 4
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP15731
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Defective activation of the ribosome quality control (RQC) pathway.
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000082545 | 1-148 | Ubiquitin-conjugating enzyme E2 4 | |||
Sequence: MSSSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATAREWTKKYAV | ||||||
Modified residue | 12 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 91 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K |
Post-translational modification
The N-terminus is blocked.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
By heat shock and cadmium.
Interaction
Subunit
Interacts with TUL1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P15731 | EGD2 P38879 | 2 | EBI-19735, EBI-6379 | |
BINARY | P15731 | MOT2 P34909 | 3 | EBI-19735, EBI-12174 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-22 | Disordered | ||||
Sequence: MSSSKRIAKELSDLERDPPTSC | ||||||
Domain | 2-148 | UBC core | ||||
Sequence: SSSKRIAKELSDLERDPPTSCSAGPVGDDLYHWQASIMGPADSPYAGGVFFLSIHFPTDYPFKPPKISFTTKIYHPNINANGNICLDILKDQWSPALTLSKVLLSICSLLTDANPDDPLVPEIAHIYKTDRPKYEATAREWTKKYAV |
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length148
- Mass (Da)16,456
- Last updated1990-04-01 v1
- Checksum8E96137D3EB20F80
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X17493 EMBL· GenBank· DDBJ | CAA35528.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X76294 EMBL· GenBank· DDBJ | CAA53942.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z35951 EMBL· GenBank· DDBJ | CAA85027.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07201.1 EMBL· GenBank· DDBJ | Genomic DNA |