P15398 · RPA1_SCHPO

Function

function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).

Catalytic activity

Features

Showing features for binding site.

116892004006008001,0001,2001,4001,600
TypeIDPosition(s)Description
Binding site63Zn2+ (UniProtKB | ChEBI)
Binding site66Zn2+ (UniProtKB | ChEBI)
Binding site73Zn2+ (UniProtKB | ChEBI)
Binding site76Zn2+ (UniProtKB | ChEBI)
Binding site643Mg2+ (UniProtKB | ChEBI); catalytic
Binding site645Mg2+ (UniProtKB | ChEBI); catalytic
Binding site647Mg2+ (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnucleolus
Cellular ComponentRNA polymerase I complex
Molecular FunctionDNA binding
Molecular FunctionDNA-directed 5'-3' RNA polymerase activity
Molecular Functionmetal ion binding
Biological Processtranscription elongation by RNA polymerase I

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA-directed RNA polymerase I subunit rpa1
  • EC number
  • Alternative names
    • DNA-directed RNA polymerase I 190 kDa polypeptide
    • DNA-directed RNA polymerase I largest subunit

Gene names

    • Name
      rpa1
    • Synonyms
      nuc1
    • ORF names
      SPBC4C3.05c

Organism names

Accessions

  • Primary accession
    P15398

Proteomes

Organism-specific databases

Subcellular Location

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000739291-1689DNA-directed RNA polymerase I subunit rpa1
Modified residue159Phosphoserine
Modified residue161Phosphoserine
Modified residue1438Phosphoserine
Modified residue1441Phosphoserine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits.
View interactors in UniProtKB
View CPX-8907 in Complex Portal

Protein-protein interaction databases

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias269-284Basic and acidic residues
Region269-295Disordered
Region1005-1017Bridging helix
Region1346-1440Disordered
Compositional bias1363-1425Basic and acidic residues

Sequence similarities

Belongs to the RNA polymerase beta' chain family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,689
  • Mass (Da)
    189,246
  • Last updated
    1998-07-15 v2
  • Checksum
    2D2D3A2DEC94A497
MNIAQPVSSEIKSVKFGIYDVDDVEKISVKQIVNPVLLDNLNHPTNGGLYDLALGPYLKNSVCATCHLDERYCPGHFGHIVLPIPAYHPLFFSQMYNLLRSTCLYCHHFKLSKVKVHLFFCRLKLLDYGLLNESEMVENVSLTEAIIKNSNGTPLEDGSDSEDSGLGHDDIAKDAATLMRIRDEFVAKSIADSRQNAHIDAQLTTLLLHERKKVVRAFYHAISSRKQCDNCQSFSPNFRKEGFAKIFEIPLSGKNLQFMEQTGKIRSDVLRDTSKKHHEDEGYDGDSDSSNESEVEGIDLFEEDPNPLKNKSKSPIAHGAKYMTSTEVRNHLRRLFVKENVVLSRLYAHKRGKPASADMFFLQNIAVPPTRFRPASKMGDEVHENIQNELLTRILQSSIQIASLSKDSTVEVNPDEKEGLERRSRAFELLINAFVQLQHDVNSLIDSNRNPSSGGQSRTVPPGIKQILEKKEGLFRKHMMGKRVNYAARSVISPDPNIETNEIGVPPVFATKLTYPEPVTLYNFNEMRNAVINGPHKWPGASHIQNEDGTLISLMPLTIEQRTALANQLLTPQSNLISSPYSYSRLINTNKKVYRHVRNGDMLILNRQPTLHKPSMMAHKARILPGEKTIRMHYANCNSYNADFDGDEMNMHFPQSTNARSEAQFIANTDSQYLVPTSGDPLRGLIQDHVVMGVWLTCKDTFYTRDEYQQLLFQALKPDETGMYGRIKTLPPAIQRPGIYWTGKQIISSVLLNLKPSDRPGLNLKSKAKVPGKYWSPDSEEGSVLFDDGELLCGILDKSSFGASAFGLVHSVHELYGPDIAGRLLSVLSRLFTAYAQMRGFTCRMDDLRLDEQGDNWRRQLLENGKSFGLEAASEYVGLSTDSPIALLNANLEEVYRDDEKLQGLDAAMKGKMNGLTSSIINKCIPDGLLTKFPYNHMQTMTVSGAKGSNVNVSQISCLLGQQELEGRRVPLMVSGKSLPSFVPYETSAKSGGFIASRFLTGIAPQEYYFHCMAGREGLIDTAVKTSRSGYLQRCLMKHLEGLCVQYDHTVRDSDGSIVQFHYGEDSLDVTKQKHLTQFEFSAKNYKSLIQKYKVKSVLSAVDSETASSYAKKALKKPYKYDPVLDKYPPSRYLGSVSEKFQRAVDEYTQKNPDKLIASKKESKLDDSLLNESKFKALMQLRYQQSLVDPGESVGVLASQSIGEPSTQMTLNTFHFAGFGAKNVTLGIPRLREIIMTASANIQTPTMTLRLNDGVSDKRASAFCKEVNKLVLSEVVRQVRVTEKISGQGSDEQSKTYAIRLDLYSRDEYQDEYGVLQEEIESTFSNRFLKILNRIIKSYLAKSKQRKSGGKDDTVPEVGQALKPLEDIDEAPIEGRAQEALEDEDNDATNEKMVSRSKQHASYEGPDEADKVALRQLKGSNKVEDVNMDEEEDEGFKSDESVSDFKERKLLEKQNTVSISERRELQLKTAKEILSNCKHLDFDYVNGEWATVELVFPINTEKLLMVSLVEKACSETVIHEIPGITRCFSKPPDSALDTVPKVITEGVNLKAIWEFYNEISMNDIYTNDIAAILRIYGVEAARNAIVHEVSSVFGVYGIAVDPRHLSLIADYMTFEGGYKAFNRMGIEYNTSPFAKMSFETTCHFLTEAALRGDVDDLSNPSSRLVVGRVGNFGTGSFDIFTPVVDSPAN

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict69in Ref. 1; CAA32887
Sequence conflict84in Ref. 1; CAA32887
Compositional bias269-284Basic and acidic residues
Sequence conflict704in Ref. 1; CAA32887
Compositional bias1363-1425Basic and acidic residues
Sequence conflict1581in Ref. 1; CAA32887
Sequence conflict1681in Ref. 1; CAA32887

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X14783
EMBL· GenBank· DDBJ
CAA32887.1
EMBL· GenBank· DDBJ
Genomic DNA
M37411
EMBL· GenBank· DDBJ
AAA35326.1
EMBL· GenBank· DDBJ
Genomic DNA
CU329671
EMBL· GenBank· DDBJ
CAA16827.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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