P15028 · FECB_ECOLI

  • Protein
    Fe(3+) dicitrate-binding periplasmic protein FecB
  • Gene
    fecB
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Part of the ABC transporter complex FecBCDE involved in citrate-dependent Fe3+ uptake (PubMed:17660286, PubMed:2651410).
Binds both iron-free and iron-loaded citrate although it binds iron-loaded citrate with a higher affinity (PubMed:26600288).
Binds different forms of Fe3+-citrate as well as citrate complexed with various representative Fe3+-mimics (Ga3+, Al3+, Sc3+ and In3+) and a representative divalent metal ion (Mg2+) (PubMed:26600288).
Can also bind various tricarboxylates in iron-free and iron-loaded form (PubMed:26600288).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing
Cellular Componentmembrane
Cellular Componentouter membrane-bounded periplasmic space
Biological Processintracellular iron ion homeostasis
Biological Processiron import into cell
Biological Processresponse to iron ion
Biological Processsiderophore-dependent iron import into cell

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Fe(3+) dicitrate-binding periplasmic protein FecB
  • Alternative names
    • Iron(III) dicitrate-binding periplasmic protein

Gene names

    • Name
      fecB
    • Ordered locus names
      b4290, JW4250

Organism names

  • Taxonomic identifier
  • Strains
    • K12
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P15028
  • Secondary accessions
    • P76816
    • Q2M625

Proteomes

Phenotypes & Variants

Features

Showing features for natural variant, mutagenesis.

TypeIDPosition(s)Description
Natural variant8in strain: B
Mutagenesis68No change in citrate-mediated Fe3+ transport.
Mutagenesis69NO change in citrate-mediated Fe3+ transport.
Mutagenesis93Retains 10% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis93Retains 17% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis93Retains 13% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis183Retains 13% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis222Retains 26% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis222Retains 32% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis222Retains 16% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis245Retains 95% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis246Retains 8% of wild-type citrate-mediated Fe3+ transport.
Mutagenesis248Retains 92% of wild-type citrate-mediated Fe3+ transport.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Miscellaneous

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-21
ChainPRO_000003182022-300Fe3+ dicitrate-binding periplasmic protein FecB

Proteomic databases

Expression

Induction

Induced 1.8-fold by hydroxyurea.

Interaction

Subunit

The complex is composed of two ATP-binding proteins (FecE), two transmembrane proteins (FecC and FecD) and a solute-binding protein (FecB) (PubMed:2651410).
Interacts with FecC and FecD (PubMed:17660286).
View interactors in UniProtKB
View CPX-4403 in Complex Portal

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain39-295Fe/B12 periplasmic-binding

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    300
  • Mass (Da)
    33,146
  • Last updated
    2003-07-19 v3
  • Checksum
    6024ED0F9C82D0EA
MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLELSFADALAAVDVIPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDLIIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQHKERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPSIGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRGIFAAERIAADTVKIFHHQPLTVVK

Sequence caution

The sequence AAA97186.1 differs from that shown. Reason: Erroneous initiation Extended N-terminus.

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict23in Ref. 1; AAA23762
Sequence conflict57in Ref. 1; AAA23762

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M26397
EMBL· GenBank· DDBJ
AAA23762.1
EMBL· GenBank· DDBJ
Genomic DNA
U14003
EMBL· GenBank· DDBJ
AAA97186.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
U00096
EMBL· GenBank· DDBJ
AAC77246.2
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE78281.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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