P14891 · HMDH1_ARATH

Function

function

Catalyzes the synthesis of mevalonate, the specific precursor of all isoprenoid compounds present in plants.

Catalytic activity

Activity regulation

Regulated at the post-translational level in response to alterations of sphingolipid and sterol biosynthetic pathways. Negatively regulated by a PP2A-dependent dephosphorylation occurring at a site different than Ser-577. Completely inhibited by mevinolin (IC50 = 12.5 nM). Reversibly inactivated by phosphorylation at Ser-577 by spinach or Brassica oleracea HMGR kinases in a cell-free system (PubMed:10318703, PubMed:7588795).
Down-regulated by KIN10 through its phosphorylation at Ser-577 (PubMed:28263378).

Pathway

Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site265Charge relay system
Active site397Charge relay system
Active site473Charge relay system
Active site571Proton donor

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentendoplasmic reticulum membrane
Molecular Functionhydroxymethylglutaryl-CoA reductase (NADPH) activity
Biological Processcoenzyme A metabolic process
Biological Processisopentenyl diphosphate biosynthetic process, mevalonate pathway
Biological Processisoprenoid biosynthetic process
Biological Processsterol biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 1
  • EC number
  • Short names
    AtHMGR1
    ; HMG-CoA reductase 1

Gene names

    • Name
      HMG1
    • Synonyms
      HMGR1
    • ORF names
      F14G6.9
      , F15M4.1
    • Ordered locus names
      At1g76490

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    P14891
  • Secondary accessions
    • Q6RW12

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Isoform Short

Endoplasmic reticulum membrane
; Multi-pass membrane protein
Note: Also observed within spherical structures located along the endoplasmic reticulum strands.

Isoform Long

Endoplasmic reticulum membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane47-69Helical
Transmembrane97-117Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Dwarfing, early senescence, and sterility. 65% lower levels in triterpenoids content and 50% lower levels in sterol content. Hmg1 and hmg2 double mutants are lethal during male gametophyte development.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis577Abolishes the inactivation of activity by KIN10.

PTM/Processing

Features

Showing features for chain, glycosylation, modified residue.

TypeIDPosition(s)Description
ChainPRO_00001144331-5923-hydroxy-3-methylglutaryl-coenzyme A reductase 1
Glycosylation16N-linked (GlcNAc...) asparagine
Glycosylation19N-linked (GlcNAc...) asparagine
Glycosylation329N-linked (GlcNAc...) asparagine
Glycosylation575N-linked (GlcNAc...) asparagine
Modified residue577Phosphoserine

Post-translational modification

Inactivated by phosphorylation at Ser-577 by KIN10 activated form (PubMed:28263378).
Probably also phosphorylated at additional sites (PubMed:10318703, PubMed:7588795).

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Found in all tissues. Isoform Short is expressed at low levels specifically in flowers. Expressed in both the tapetum and microspores.

Induction

Down-regulated by light in immature leaves, but not in roots. Not regulated by myriocin, squalestatin or terbinafine.

Gene expression databases

Interaction

Subunit

Interacts (via N-terminus) with B''ALPHA and B''BETA.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region1-45Disordered
Compositional bias10-26Polar residues
Region118-171Linker
Region172-592Catalytic

Domain

The N-terminal domain (1-178) of the short isoform is necessary and sufficient for directing the protein to the endoplasmic reticulum and to spherical structures.

Sequence similarities

Belongs to the HMG-CoA reductase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative initiation.

P14891-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Name
    Short
  • Synonyms
    HMGR1S
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Length
    592
  • Mass (Da)
    63,598
  • Last updated
    1990-04-01 v1
  • Checksum
    7EE10127460D3573
MDLRRRPPKPPVTNNNNSNGSFRSYQPRTSDDDHRRRATTIAPPPKASDALPLPLYLTNAVFFTLFFSVAYYLLHRWRDKIRYNTPLHVVTITELGAIIALIASFIYLLGFFGIDFVQSFISRASGDAWDLADTIDDDDHRLVTCSPPTPIVSVAKLPNPEPIVTESLPEEDEEIVKSVIDGVIPSYSLESRLGDCKRAASIRREALQRVTGRSIEGLPLDGFDYESILGQCCEMPVGYIQIPVGIAGPLLLDGYEYSVPMATTEGCLVASTNRGCKAMFISGGATSTVLKDGMTRAPVVRFASARRASELKFFLENPENFDTLAVVFNRSSRFARLQSVKCTIAGKNAYVRFCCSTGDAMGMNMVSKGVQNVLEYLTDDFPDMDVIGISGNFCSDKKPAAVNWIEGRGKSVVCEAVIRGEIVNKVLKTSVAALVELNMLKNLAGSAVAGSLGGFNAHASNIVSAVFIATGQDPAQNVESSQCITMMEAINDGKDIHISVTMPSIEVGTVGGGTQLASQSACLNLLGVKGASTESPGMNARRLATIVAGAVLAGELSLMSAIAAGQLVRSHMKYNRSSRDISGATTTTTTTT

P14891-2

  • Name
    Long
  • Synonyms
    HMGR1L
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-1: M → MKKKQAGPQQTCEFVSYKTLLISPSHLSRHLTTSLLSPLSPPWRDYSFPPM

Features

Showing features for alternative sequence, compositional bias.

TypeIDPosition(s)Description
Alternative sequenceVSP_0412821in isoform Long
Compositional bias10-26Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X15032
EMBL· GenBank· DDBJ
CAA33139.1
EMBL· GenBank· DDBJ
mRNA
J04537
EMBL· GenBank· DDBJ
AAA76821.1
EMBL· GenBank· DDBJ
mRNA
L19261
EMBL· GenBank· DDBJ
AAA32814.1
EMBL· GenBank· DDBJ
Genomic DNA
AY488113
EMBL· GenBank· DDBJ
AAR83122.1
EMBL· GenBank· DDBJ
mRNA
AC012394
EMBL· GenBank· DDBJ
AAF16652.1
EMBL· GenBank· DDBJ
Genomic DNA
AC015450
EMBL· GenBank· DDBJ
AAG51957.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE35849.1
EMBL· GenBank· DDBJ
Genomic DNA
AF385690
EMBL· GenBank· DDBJ
AAK60283.1
EMBL· GenBank· DDBJ
mRNA
BT000703
EMBL· GenBank· DDBJ
AAN31847.1
EMBL· GenBank· DDBJ
mRNA
BT010468
EMBL· GenBank· DDBJ
AAQ65091.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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