P14874 · ILVB2_BRANA
- ProteinAcetolactate synthase 2, chloroplastic
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids637 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
Miscellaneous
Acetolactate synthase is the target enzyme for sulfonylurea and imidazolinone herbicides.
Catalytic activity
- H+ + 2 pyruvate = (2S)-2-acetolactate + CO2
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 1 thiamine pyrophosphate per subunit.
Pathway
Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 120 | thiamine diphosphate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 222 | FAD (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 329-350 | FAD (UniProtKB | ChEBI) | ||||
Sequence: HGTVYANYAVEYSDLLLAFGVR | ||||||
Binding site | 372-391 | FAD (UniProtKB | ChEBI) | ||||
Sequence: DIDSTEIGKNKTPHVSVCCD | ||||||
Binding site | 513 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 540 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | acetolactate synthase complex | |
Cellular Component | chloroplast | |
Molecular Function | acetolactate synthase activity | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | thiamine pyrophosphate binding | |
Biological Process | isoleucine biosynthetic process | |
Biological Process | response to herbicide | |
Biological Process | valine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAcetolactate synthase 2, chloroplastic
- EC number
- Alternative names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Brassiceae > Brassica
Accessions
- Primary accessionP14874
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-73 | Chloroplast | ||||
Sequence: MASFSFFGTIPSSPTKASVFSLPVSVTTLPSFPRRRATRVSVSANSKKDQDRTASRRENPSTFSSKYAPNVPR | ||||||
Chain | PRO_0000035657 | 74-637 | Acetolactate synthase 2, chloroplastic | |||
Sequence: SGADILVEALERQGVDVVFAYPGGASMEIHQALTRSNTIRNVLPRHEQGGIFAAEGYARSSGKPGICIATSGPGAMNLVSGLADALFDSVPLIAITGQVPRRMIGTMAFQETPVVEVTRTITKHNYLVMEVDDIPRIVREAFFLATSVRPGPVLIDVPKDVQQQFAIPNWEQPMRLPLYMSTMPKPPKVSHLEQILRLVSESKRPVLYVGGGCLNSSEELRRFVELTGIPVASTFMGLGSYPCDDEEFSLQMLGMHGTVYANYAVEYSDLLLAFGVRFDDRVTGKLEAFASRAKIVHIDIDSTEIGKNKTPHVSVCCDVQLALQGMNEVLENRRDVLDFGEWRCELNEQRLKFPLRYKTFGEEIPPQYAIQLLDELTDGKAIITTGVGQHQMWAAQFYRFKKPRQWLSSGGLGAMGFGLPAAMGAAIANPGAVVVDIDGDGSFIMNIQELATIRVENLPVKVLLINNQHLGMVLQWEDHFYAANRADSFLGDPANPEAVFPDMLLFAASCGIPAARVTRREDLREAIQTMLDTPGPFLLDVVCPHQDHVLPLIPSGGTFKDIIV |
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 35-69 | Disordered | ||||
Sequence: RRATRVSVSANSKKDQDRTASRRENPSTFSSKYAP | ||||||
Region | 462-542 | Thiamine pyrophosphate binding | ||||
Sequence: QHQMWAAQFYRFKKPRQWLSSGGLGAMGFGLPAAMGAAIANPGAVVVDIDGDGSFIMNIQELATIRVENLPVKVLLINNQH |
Sequence similarities
Belongs to the TPP enzyme family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length637
- Mass (Da)69,970
- Last updated1990-04-01 v1
- Checksum9FC6A6E8124C2455
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z11525 EMBL· GenBank· DDBJ | CAA77614.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X16708 EMBL· GenBank· DDBJ | CAA34680.1 EMBL· GenBank· DDBJ | Genomic DNA |