P14647 · RDRP_BPQBE
- ProteinRNA-directed RNA polymerase subunit beta
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids589 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
This is the catalytic subunit of the viral RNA-dependent RNA polymerase complex. This complex is involved in viral RNA replication that produces +-stranded genomes via a complementary, --stranded intermediate. Binds RNA cooperatively with the host ribosomal protein S1.
Miscellaneous
In order to produce high amounts of RNA polymerase catalytic core, a fusion protein consisting of tsf-tufB-replicase with a cleavable linker between tufB and the viral replicase subunit is frequently used.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Note: Binds 2 Mg2+ per subunit, Ca2+ is used in crystallization to prevent RNA polymerase activity.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 274 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 274 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 359 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 359 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 360 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 360 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | viral RNA genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA-directed RNA polymerase subunit beta
- EC number
- Alternative names
Organism names
- Strains
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Lenarviricota > Leviviricetes > Norzivirales > Fiersviridae > Qubevirus
- Virus hosts
Accessions
- Primary accessionP14647
- Secondary accessions
Proteomes
Phenotypes & Variants
Features
Showing features for natural variant, mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 71 | in strain: QB_1 | ||||
Sequence: L → F | ||||||
Mutagenesis | 78 | Loss of RNA polymerase activity. | ||||
Sequence: K → A | ||||||
Natural variant | 130 | in strain: QB_ancestral, QB_1, QB_2, Qbeta_1_FR and Qbeta_2_FR | ||||
Sequence: M → I | ||||||
Mutagenesis | 132 | Complete loss of infectivity; when associated with M-133. | ||||
Sequence: R → M | ||||||
Mutagenesis | 133 | Complete loss of infectivity; when associated with M-132. | ||||
Sequence: R → M | ||||||
Mutagenesis | 134 | Complete loss of infectivity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 137 | Complete loss of infectivity. | ||||
Sequence: K → M | ||||||
Mutagenesis | 153 | Loss of RNA polymerase activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 164 | 80% loss of RNA polymerase activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 168 | 80% loss of RNA polymerase activity. | ||||
Sequence: H → A | ||||||
Natural variant | 198 | |||||
Sequence: I → T | ||||||
Mutagenesis | 214 | Loss of RNA polymerase activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 220 | Loss of RNA polymerase activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 241 | Decreased initiation of RNA polymerase activity. | ||||
Sequence: R → A | ||||||
Natural variant | 251 | in strain: QB_1 and Qbeta_2_FR | ||||
Sequence: L → R | ||||||
Mutagenesis | 274 | Loss of RNA polymerase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 279 | Loss of RNA polymerase activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 329 | Decreased initiation of RNA polymerase activity. | ||||
Sequence: F → A | ||||||
Mutagenesis | 345 | Almost complete loss of infectivity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 348 | 80% loss of infectivity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 350 | 95% loss of infectivity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 357 | Decreased initiation of RNA polymerase activity. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 359 | Loss of RNA polymerase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 360 | Loss of RNA polymerase activity. | ||||
Sequence: D → A | ||||||
Mutagenesis | 395 | Loss of RNA polymerase activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 411 | 60% loss of RNA polymerase activity. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 413 | Loss of RNA polymerase activity. | ||||
Sequence: R → A | ||||||
Natural variant | 418 | in strain: QB_1 and Qbeta_2_FR | ||||
Sequence: S → G | ||||||
Natural variant | 500 | in strain: QB_2 and Qbeta_2_FR | ||||
Sequence: D → G | ||||||
Mutagenesis | 511 | 50% loss of RNA polymerase activity. | ||||
Sequence: Y → A | ||||||
Mutagenesis | 549 | 25% loss of RNA polymerase activity. | ||||
Sequence: N → A | ||||||
Mutagenesis | 549 | 50% loss of RNA polymerase activity. | ||||
Sequence: N → G |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000164855 | 1-589 | RNA-directed RNA polymerase subunit beta | |||
Sequence: MSKTASSRNSLSAQLRRAANTRIEVEGNLALSIANDLLLAYGQSPFNSEAECISFSPRFDGTPDDFRINYLKAEIMSKYDDFSLGIDTEAVAWEKFLAAEAECALTNARLYRPDYSEDFNFSLGESCIHMARRKIAKLIGDVPSVEGMLRHCRFSGGATTTNNRSYGHPSFKFALPQACTPRALKYVLALRASTHFDIRISDISPFNKAVTVPKNSKTDRCIAIEPGWNMFFQLGIGGILRDRLRCWGIDLNDQTINQRRAHEGSVTNNLATVDLSAASDSISLALCELLLPPGWFEVLMDLRSPKGRLPDGSVVTYEKISSMGNGYTFELESLIFASLARSVCEILDLDSSEVTVYGDDIILPSCAVPALREVFKYVGFTTNTKKTFSEGPFRESCGKHYYSGVDVTPFYIRHRIVSPADLILVLNNLYRWATIDGVWDPRAHSVYLKYRKLLPKQLQRNTIPDGYGDGALVGSVLINPFAKNRGWIRYVPVITDHTRDRERAELGSYLYDLFSRCLSESNDGLPLRGPSGCDSADLFAIDQLICRSNPTKISRSTGKFDIQYIACSSRVLAPYGVFQGTKVASLHEA |
Interaction
Subunit
Homodimer; the replicase complex can dimerize. Part of the viral RNA-dependent RNA polymerase complex, the other subunits are the host ribosomal protein S1, EF-Tu and EF-Ts. S1 is needed for the initiation of genomic RNA +-strand replication.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | P14647 | tsf P0A6P1 | 2 | EBI-9010000, EBI-301164 | |
XENO | P14647 | tufA P0CE47 | 2 | EBI-9010000, EBI-301077 |
Protein-protein interaction databases
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length589
- Mass (Da)65,531
- Last updated2017-02-15 v2
- Checksum8C02DB50F7202DEF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X14764 EMBL· GenBank· DDBJ | CAA32872.1 EMBL· GenBank· DDBJ | mRNA | ||
AY099114 EMBL· GenBank· DDBJ | AAM33128.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
GQ153928 EMBL· GenBank· DDBJ | ACY07225.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
GQ153929 EMBL· GenBank· DDBJ | ACY07229.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
AB971354 EMBL· GenBank· DDBJ | BAP18765.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
JF719735 EMBL· GenBank· DDBJ | AEQ25543.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
JF719736 EMBL· GenBank· DDBJ | AEQ25547.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
GQ153931 EMBL· GenBank· DDBJ | ACY07237.1 EMBL· GenBank· DDBJ | Genomic RNA | ||
M24876 EMBL· GenBank· DDBJ | AAA50307.1 EMBL· GenBank· DDBJ | Genomic RNA |