P14647 · RDRP_BPQBE

Function

function

This is the catalytic subunit of the viral RNA-dependent RNA polymerase complex. This complex is involved in viral RNA replication that produces +-stranded genomes via a complementary, --stranded intermediate. Binds RNA cooperatively with the host ribosomal protein S1.

Miscellaneous

In order to produce high amounts of RNA polymerase catalytic core, a fusion protein consisting of tsf-tufB-replicase with a cleavable linker between tufB and the viral replicase subunit is frequently used.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 Mg2+ per subunit, Ca2+ is used in crystallization to prevent RNA polymerase activity.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site274Mg2+ 1 (UniProtKB | ChEBI)
Binding site274Mg2+ 2 (UniProtKB | ChEBI)
Binding site359Mg2+ 1 (UniProtKB | ChEBI)
Binding site359Mg2+ 2 (UniProtKB | ChEBI)
Binding site360Mg2+ 1 (UniProtKB | ChEBI)
Binding site360Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionmetal ion binding
Molecular Functionnucleotide binding
Molecular FunctionRNA binding
Molecular FunctionRNA-dependent RNA polymerase activity
Biological Processviral RNA genome replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RNA-directed RNA polymerase subunit beta
  • EC number
  • Alternative names
    • RNA replicase beta chain
    • RNA-directed RNA polymerase subunit II

Organism names

Accessions

  • Primary accession
    P14647
  • Secondary accessions
    • D0U1F4
    • D0U1F8
    • D0U1G6
    • G4WZR0
    • G4WZR4
    • Q8LTE0

Proteomes

Phenotypes & Variants

Features

Showing features for natural variant, mutagenesis.

TypeIDPosition(s)Description
Natural variant71in strain: QB_1
Mutagenesis78Loss of RNA polymerase activity.
Natural variant130in strain: QB_ancestral, QB_1, QB_2, Qbeta_1_FR and Qbeta_2_FR
Mutagenesis132Complete loss of infectivity; when associated with M-133.
Mutagenesis133Complete loss of infectivity; when associated with M-132.
Mutagenesis134Complete loss of infectivity.
Mutagenesis137Complete loss of infectivity.
Mutagenesis153Loss of RNA polymerase activity.
Mutagenesis16480% loss of RNA polymerase activity.
Mutagenesis16880% loss of RNA polymerase activity.
Natural variant198
Mutagenesis214Loss of RNA polymerase activity.
Mutagenesis220Loss of RNA polymerase activity.
Mutagenesis241Decreased initiation of RNA polymerase activity.
Natural variant251in strain: QB_1 and Qbeta_2_FR
Mutagenesis274Loss of RNA polymerase activity.
Mutagenesis279Loss of RNA polymerase activity.
Mutagenesis329Decreased initiation of RNA polymerase activity.
Mutagenesis345Almost complete loss of infectivity.
Mutagenesis34880% loss of infectivity.
Mutagenesis35095% loss of infectivity.
Mutagenesis357Decreased initiation of RNA polymerase activity.
Mutagenesis359Loss of RNA polymerase activity.
Mutagenesis360Loss of RNA polymerase activity.
Mutagenesis395Loss of RNA polymerase activity.
Mutagenesis41160% loss of RNA polymerase activity.
Mutagenesis413Loss of RNA polymerase activity.
Natural variant418in strain: QB_1 and Qbeta_2_FR
Natural variant500in strain: QB_2 and Qbeta_2_FR
Mutagenesis51150% loss of RNA polymerase activity.
Mutagenesis54925% loss of RNA polymerase activity.
Mutagenesis54950% loss of RNA polymerase activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001648551-589RNA-directed RNA polymerase subunit beta

Interaction

Subunit

Homodimer; the replicase complex can dimerize. Part of the viral RNA-dependent RNA polymerase complex, the other subunits are the host ribosomal protein S1, EF-Tu and EF-Ts. S1 is needed for the initiation of genomic RNA +-strand replication.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
XENO P14647tsf P0A6P12EBI-9010000, EBI-301164
XENO P14647tufA P0CE472EBI-9010000, EBI-301077

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain259-391RdRp catalytic

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    589
  • Mass (Da)
    65,531
  • Last updated
    2017-02-15 v2
  • Checksum
    8C02DB50F7202DEF
MSKTASSRNSLSAQLRRAANTRIEVEGNLALSIANDLLLAYGQSPFNSEAECISFSPRFDGTPDDFRINYLKAEIMSKYDDFSLGIDTEAVAWEKFLAAEAECALTNARLYRPDYSEDFNFSLGESCIHMARRKIAKLIGDVPSVEGMLRHCRFSGGATTTNNRSYGHPSFKFALPQACTPRALKYVLALRASTHFDIRISDISPFNKAVTVPKNSKTDRCIAIEPGWNMFFQLGIGGILRDRLRCWGIDLNDQTINQRRAHEGSVTNNLATVDLSAASDSISLALCELLLPPGWFEVLMDLRSPKGRLPDGSVVTYEKISSMGNGYTFELESLIFASLARSVCEILDLDSSEVTVYGDDIILPSCAVPALREVFKYVGFTTNTKKTFSEGPFRESCGKHYYSGVDVTPFYIRHRIVSPADLILVLNNLYRWATIDGVWDPRAHSVYLKYRKLLPKQLQRNTIPDGYGDGALVGSVLINPFAKNRGWIRYVPVITDHTRDRERAELGSYLYDLFSRCLSESNDGLPLRGPSGCDSADLFAIDQLICRSNPTKISRSTGKFDIQYIACSSRVLAPYGVFQGTKVASLHEA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X14764
EMBL· GenBank· DDBJ
CAA32872.1
EMBL· GenBank· DDBJ
mRNA
AY099114
EMBL· GenBank· DDBJ
AAM33128.1
EMBL· GenBank· DDBJ
Genomic RNA
GQ153928
EMBL· GenBank· DDBJ
ACY07225.1
EMBL· GenBank· DDBJ
Genomic RNA
GQ153929
EMBL· GenBank· DDBJ
ACY07229.1
EMBL· GenBank· DDBJ
Genomic RNA
AB971354
EMBL· GenBank· DDBJ
BAP18765.1
EMBL· GenBank· DDBJ
Genomic RNA
JF719735
EMBL· GenBank· DDBJ
AEQ25543.1
EMBL· GenBank· DDBJ
Genomic RNA
JF719736
EMBL· GenBank· DDBJ
AEQ25547.1
EMBL· GenBank· DDBJ
Genomic RNA
GQ153931
EMBL· GenBank· DDBJ
ACY07237.1
EMBL· GenBank· DDBJ
Genomic RNA
M24876
EMBL· GenBank· DDBJ
AAA50307.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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