P14404 · MECOM_MOUSE
- ProteinHistone-lysine N-methyltransferase MECOM
- GeneMecom
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1232 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Isoform 1
Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.
Isoform 3
Displays histone methyltransferase activity and monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro. Probably catalyzes the monomethylation of free histone H3 in the cytoplasm which is then transported to the nucleus and incorporated into nucleosomes where SUV39H methyltransferases use it as a substrate to catalyze histone H3 'Lys-9' trimethylation. Likely to be one of the primary histone methyltransferases along with PRDM16 that direct cytoplasmic H3K9me1 methylation.
Catalytic activity
- L-lysyl9-[histone H3] + S-adenosyl-L-methionine = H+ + N6-methyl-L-lysyl9-[histone H3] + S-adenosyl-L-homocysteine
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHistone-lysine N-methyltransferase MECOM
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP14404
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Mice develop until 9.5 dpc but die before 11.5 dpc. At 10.5 dpc embryos display multiple malformations associated with hypocellularity and reduced body size. Required for neural, heart and paraxial mesenchyme.
PTM/Processing
Features
Showing features for chain, cross-link, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000047274 | 1-1232 | Histone-lysine N-methyltransferase MECOM | |||
Sequence: MRSKGRARKLATSNECAYGNYPEIPLEEMPDADADGITSVPSLHIQEPCSPATSSESFTPKEGSPYKAPIYIPDDIPIPDEFELRESTMPGAGLGIWTKRKIEIGEKFGPYMGEQRSDLKDSSYGWEILDEFCNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFMKSEEDPHEPMAPDIHEERQHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLREIHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGTNRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASPPVKGLSSTEQSNKCQSPLLTHPQILPATQDILKALSKHPPVGDNKPVELLPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDLESDKEKCKENGKMFKDKVSPLQNLASITNKKEHNNHSVFSASVEEQSAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTSGPSKPSGTPATSQDQPLDLSMGSRGRASGTKLTEPRKNHVFGEKKGSNMDTRPSSDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQMSAIENMAEKLESFSALKPEASELLQSVPSMFSFRAPPNTLPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESAGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNMEERMNGSHFKDKKALATSQNSDLLDDEEVEDEVLLDEEDEDNDIPGKPRKELGVTRLDEEIPEDDYEEAGALEMSCKASPVRYKEEDYKSGLSALDHIRHFTDSLKMREMEENQYTDAELSSISSSHVPEELKQTLHRKSKSQAYAMMLSLSDKDSLHPTSHSSSNVWHSMARAAAESSAIQSISHV | ||||||
Cross-link | 101 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 192 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 294 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 369 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 376 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 432 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 525 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 545 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 549 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 557 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 624 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 626 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 637 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 665 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 687 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 723 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 728 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 733 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 734 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 737 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 742 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 751 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 754 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 762 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 789 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 802 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 803 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 837 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 846 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 848 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 879 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 1020 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Modified residue | 1039 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 1041 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 1055 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 1058 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 1122 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 1129 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 1134 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 1151 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 1178 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K | ||||||
Cross-link | 1186 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) | ||||
Sequence: K |
Post-translational modification
May be acetylated by CREBBP and KAT2B.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Expressed at 8.5 dpc in the anterior section of the primary head folds. Ubiquitously expressed at 9.5 dpc with higher expression in forebrain, mesenchyme of the branchial arches, nasal pits, limb buds and mesonephric ducts. Also detected at 10.5 dpc in hindbrain and lateral region of the neural tube.
Gene expression databases
Interaction
Subunit
Isoform 1
Homooligomer. Interacts with CTBP1. Interacts with SMAD3 (via MH2 domain); the interaction is direct. Interacts with SMAD4; through interaction with SMAD3. Interacts with CREBBP, KAT2B and histone deacetylases. Interacts with MAPK8 and MAPK9; inhibits JNK signaling (By similarity).
Interacts with SUV39H1 (via SET domain); enhances MECOM transcriptional repression activity
Interacts with SUV39H1 (via SET domain); enhances MECOM transcriptional repression activity
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P14404 | Fos P01101 | 2 | EBI-1994523, EBI-4288185 | |
BINARY | P14404 | Mbd3 Q9Z2D8 | 4 | EBI-1994523, EBI-1994548 | |
BINARY | P14404 | Mbd3 Q9Z2D8-1 | 5 | EBI-1994523, EBI-1994598 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, zinc finger, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 22-68 | Disordered | ||||
Sequence: PEIPLEEMPDADADGITSVPSLHIQEPCSPATSSESFTPKEGSPYKA | ||||||
Compositional bias | 43-61 | Polar residues | ||||
Sequence: LHIQEPCSPATSSESFTPK | ||||||
Domain | 80-192 | SET | ||||
Sequence: DEFELRESTMPGAGLGIWTKRKIEIGEKFGPYMGEQRSDLKDSSYGWEILDEFCNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFMK | ||||||
Region | 191-442 | Interaction with SUV39H1 and probably MAPK9 and SMAD3 | ||||
Sequence: MKSEEDPHEPMAPDIHEERQHRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDLQQNLESESDLREIHGNQDCKECDRVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFG | ||||||
Zinc finger | 211-238 | C2H2-type 1 | ||||
Sequence: HRCEDCDQLFESKAELADHQKFPCSTPH | ||||||
Zinc finger | 265-287 | C2H2-type 2 | ||||
Sequence: QDCKECDRVFPDLQSLEKHMLSH | ||||||
Zinc finger | 293-315 | C2H2-type 3 | ||||
Sequence: YKCDQCPKAFNWKSNLIRHQMSH | ||||||
Zinc finger | 321-344 | C2H2-type 4 | ||||
Sequence: YECENCAKVFTDPSNLQRHIRSQH | ||||||
Zinc finger | 350-372 | C2H2-type 5 | ||||
Sequence: HACPECGKTFATSSGLKQHKHIH | ||||||
Zinc finger | 378-400 | C2H2-type 6 | ||||
Sequence: FICEVCHKSYTQFSNLCRHKRMH | ||||||
Zinc finger | 407-429 | C2H2-type 7; atypical | ||||
Sequence: IKCKDCGQMFSTTSSLNKHRRFC | ||||||
Region | 548-622 | Disordered | ||||
Sequence: SKHPPVGDNKPVELLPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDLESDKEKCKENGKMFK | ||||||
Compositional bias | 561-581 | Basic and acidic residues | ||||
Sequence: LLPERSSEERPLEKISDQSES | ||||||
Compositional bias | 582-602 | Polar residues | ||||
Sequence: SDLDDVSTPSGSDLETTSGSD | ||||||
Compositional bias | 604-622 | Basic and acidic residues | ||||
Sequence: ESDLESDKEKCKENGKMFK | ||||||
Motif | 611-624 | Nuclear localization signal | ||||
Sequence: KEKCKENGKMFKDK | ||||||
Region | 720-823 | Disordered | ||||
Sequence: LPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTSGPSKPSGTPATSQDQPLDLSMGSRGRASGTKLTEPRKNHVFGEKKGSNMDTRPSSDGSLQHARPT | ||||||
Motif | 743-747 | CTBP-binding motif 1 | ||||
Sequence: PFDLT | ||||||
Compositional bias | 759-777 | Polar residues | ||||
Sequence: GPSKPSGTPATSQDQPLDL | ||||||
Motif | 774-778 | CTBP-binding motif 2 | ||||
Sequence: PLDLS | ||||||
Compositional bias | 788-808 | Basic and acidic residues | ||||
Sequence: TKLTEPRKNHVFGEKKGSNMD | ||||||
Zinc finger | 914-936 | C2H2-type 8 | ||||
Sequence: YTCRYCGKIFPRSANLTRHLRTH | ||||||
Zinc finger | 942-965 | C2H2-type 9 | ||||
Sequence: YRCKYCDRSFSISSNLQRHVRNIH | ||||||
Zinc finger | 971-993 | C2H2-type 10 | ||||
Sequence: FKCHLCDRCFGQQTNLDRHLKKH | ||||||
Compositional bias | 1032-1047 | Polar residues | ||||
Sequence: IGNSNHGSQSPRNMEE | ||||||
Region | 1032-1107 | Disordered | ||||
Sequence: IGNSNHGSQSPRNMEERMNGSHFKDKKALATSQNSDLLDDEEVEDEVLLDEEDEDNDIPGKPRKELGVTRLDEEIP | ||||||
Compositional bias | 1068-1086 | Acidic residues | ||||
Sequence: LLDDEEVEDEVLLDEEDED | ||||||
Compositional bias | 1087-1104 | Basic and acidic residues | ||||
Sequence: NDIPGKPRKELGVTRLDE |
Domain
Both zinc finger regions are required for the transcriptional activation of PBX1.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative promoter usage & Alternative splicing.
P14404-2
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name3
- Length1,232
- Mass (Da)138,100
- Last updated2018-03-28 v2
- Checksum044BB6F19DBD1403
P14404-1
- Name1
- Differences from canonical
- 1-190: Missing
P14404-3
- Name4
- SynonymsMds1
- NoteProduced by alternative promoter usage. May be due to intron retention.
Computationally mapped potential isoform sequences
There are 11 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F6SZX1 | F6SZX1_MOUSE | Mecom | 116 | ||
A0A5H1ZRN1 | A0A5H1ZRN1_MOUSE | Mecom | 994 | ||
Q8CCA6 | Q8CCA6_MOUSE | Mecom | 795 | ||
G3UZT5 | G3UZT5_MOUSE | Mecom | 1051 | ||
G3UYK2 | G3UYK2_MOUSE | Mecom | 949 | ||
G3UYN3 | G3UYN3_MOUSE | Mecom | 45 | ||
G3UZ13 | G3UZ13_MOUSE | Mecom | 718 | ||
G3UYY9 | G3UYY9_MOUSE | Mecom | 606 | ||
G3UY46 | G3UY46_MOUSE | Mecom | 52 | ||
G3UWJ0 | G3UWJ0_MOUSE | Mecom | 843 | ||
O35700 | O35700_MOUSE | Mecom | 949 |
Sequence caution
Features
Showing features for alternative sequence, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_059487 | 1-190 | in isoform 1 | |||
Sequence: Missing | ||||||
Compositional bias | 43-61 | Polar residues | ||||
Sequence: LHIQEPCSPATSSESFTPK | ||||||
Alternative sequence | VSP_059488 | 128-129 | in isoform 4 | |||
Sequence: IL → NR | ||||||
Alternative sequence | VSP_059489 | 130-1232 | in isoform 4 | |||
Sequence: Missing | ||||||
Compositional bias | 561-581 | Basic and acidic residues | ||||
Sequence: LLPERSSEERPLEKISDQSES | ||||||
Compositional bias | 582-602 | Polar residues | ||||
Sequence: SDLDDVSTPSGSDLETTSGSD | ||||||
Compositional bias | 604-622 | Basic and acidic residues | ||||
Sequence: ESDLESDKEKCKENGKMFK | ||||||
Compositional bias | 759-777 | Polar residues | ||||
Sequence: GPSKPSGTPATSQDQPLDL | ||||||
Compositional bias | 788-808 | Basic and acidic residues | ||||
Sequence: TKLTEPRKNHVFGEKKGSNMD | ||||||
Compositional bias | 1032-1047 | Polar residues | ||||
Sequence: IGNSNHGSQSPRNMEE | ||||||
Compositional bias | 1068-1086 | Acidic residues | ||||
Sequence: LLDDEEVEDEVLLDEEDED | ||||||
Compositional bias | 1087-1104 | Basic and acidic residues | ||||
Sequence: NDIPGKPRKELGVTRLDE |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ010015 EMBL· GenBank· DDBJ | CAA08971.1 EMBL· GenBank· DDBJ | mRNA | ||
M21829 EMBL· GenBank· DDBJ | AAA40581.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AC119995 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL683891 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL691419 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL772145 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL929377 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |