P14338 · POLG_DEN22
- ProteinGenome polyprotein
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids495 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Envelope protein E
Binds to host cell surface receptor and mediates fusion between viral and cellular membranes. Envelope protein is synthesized in the endoplasmic reticulum in the form of heterodimer with protein prM. They play a role in virion budding in the ER, and the newly formed immature particle is covered with 60 spikes composed of heterodimer between precursor prM and envelope protein E. The virion is transported to the Golgi apparatus where the low pH causes dissociation of PrM-E heterodimers and formation of E homodimers. prM-E cleavage is inefficient, and many virions are only partially matured. These uncleaved prM would play a role in immune evasion.
Miscellaneous
Isolate M2 comes from a patient with dengue shock syndrome.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | host cell endoplasmic reticulum membrane | |
Cellular Component | membrane | |
Cellular Component | viral envelope | |
Cellular Component | viral nucleocapsid | |
Cellular Component | virion membrane | |
Molecular Function | protein dimerization activity | |
Biological Process | clathrin-dependent endocytosis of virus by host cell | |
Biological Process | fusion of virus membrane with host endosome membrane | |
Biological Process | symbiont-mediated suppression of host innate immune response | |
Biological Process | virion attachment to host cell | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameGenome polyprotein
- Cleaved into 1 chains
Organism names
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Kitrinoviricota > Flasuviricetes > Amarillovirales > Flaviviridae > Orthoflavivirus > Orthoflavivirus denguei > Dengue virus
- Virus hosts
Accessions
- Primary accessionP14338
Subcellular Location
UniProt Annotation
GO Annotation
Envelope protein E
Virion membrane ; Multi-pass membrane protein
Host endoplasmic reticulum membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-445 | Extracellular | ||||
Sequence: MRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKQPATLRKYCIEAKLTNTTTESRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGCGLFGKGGIVTCAMFTCQKNMEGKIVQPENLEYTIVVTPHSGEEHAVGNDTGKHGKEIKITPQSSITEAELTGYGTVTMDCSPRTGLDFNEMVLLQMENKAWLVHRQWFLDLPLPWLPGADTQGSKLDQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKVVEEIAETQHGTIVIRVQYEGDGSPCKIPLEIMDLDNRHVLGRLITVNPIVTEKDSPVNVEAEPPLGDSYIIIGVEPGQLKLNWFKKGSSIGQMFETTMIRAKRMAILGDTAWDFRSLGGVFTSIGKALHQVFGAIYG | ||||||
Transmembrane | 446-466 | Helical | ||||
Sequence: AAFSGVSWTMKILIGVIITWI | ||||||
Topological domain | 467-472 | Cytoplasmic | ||||
Sequence: GMNSRS | ||||||
Transmembrane | 473-493 | Helical | ||||
Sequence: TSLSVSLVLVGIVTLYLGVMV | ||||||
Topological domain | 494-495 | Extracellular | ||||
Sequence: QA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000405208 | 1-495 | Genome polyprotein | |||
Sequence: MRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKQPATLRKYCIEAKLTNTTTESRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGCGLFGKGGIVTCAMFTCQKNMEGKIVQPENLEYTIVVTPHSGEEHAVGNDTGKHGKEIKITPQSSITEAELTGYGTVTMDCSPRTGLDFNEMVLLQMENKAWLVHRQWFLDLPLPWLPGADTQGSKLDQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKVVEEIAETQHGTIVIRVQYEGDGSPCKIPLEIMDLDNRHVLGRLITVNPIVTEKDSPVNVEAEPPLGDSYIIIGVEPGQLKLNWFKKGSSIGQMFETTMIRAKRMAILGDTAWDFRSLGGVFTSIGKALHQVFGAIYGAAFSGVSWTMKILIGVIITWIGMNSRSTSLSVSLVLVGIVTLYLGVMVQA | ||||||
Chain | PRO_0000037915 | 1-495 | Envelope protein E | |||
Sequence: MRCIGISNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFELIKTEAKQPATLRKYCIEAKLTNTTTESRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGCGLFGKGGIVTCAMFTCQKNMEGKIVQPENLEYTIVVTPHSGEEHAVGNDTGKHGKEIKITPQSSITEAELTGYGTVTMDCSPRTGLDFNEMVLLQMENKAWLVHRQWFLDLPLPWLPGADTQGSKLDQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKCRLRMDKLQLKGMSYSMCTGKFKVVEEIAETQHGTIVIRVQYEGDGSPCKIPLEIMDLDNRHVLGRLITVNPIVTEKDSPVNVEAEPPLGDSYIIIGVEPGQLKLNWFKKGSSIGQMFETTMIRAKRMAILGDTAWDFRSLGGVFTSIGKALHQVFGAIYGAAFSGVSWTMKILIGVIITWIGMNSRSTSLSVSLVLVGIVTLYLGVMVQA | ||||||
Disulfide bond | 3↔30 | |||||
Sequence: CIGISNRDFVEGVSGGSWVDIVLEHGSC | ||||||
Disulfide bond | 60↔121 | |||||
Sequence: CIEAKLTNTTTESRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGCGLFGKGGIVTCAMFTC | ||||||
Glycosylation | 67 | N-linked (GlcNAc...) asparagine; by host | ||||
Sequence: N | ||||||
Disulfide bond | 74↔105 | |||||
Sequence: CPTQGEPSLNEEQDKRFVCKHSMVDRGWGNGC | ||||||
Disulfide bond | 92↔116 | |||||
Sequence: CKHSMVDRGWGNGCGLFGKGGIVTC | ||||||
Glycosylation | 153 | N-linked (GlcNAc...) asparagine; by host | ||||
Sequence: N | ||||||
Disulfide bond | 185↔285 | |||||
Sequence: CSPRTGLDFNEMVLLQMENKAWLVHRQWFLDLPLPWLPGADTQGSKLDQKETLVTFKNPHAKKQDVVVLGSQEGAMHTALTGATEIQMSSGNLLFTGHLKC | ||||||
Disulfide bond | 302↔333 | |||||
Sequence: CTGKFKVVEEIAETQHGTIVIRVQYEGDGSPC |
Post-translational modification
Envelope protein E
N-glycosylated.
Genome polyprotein
Specific enzymatic cleavages in vivo yield mature proteins. Cleavages in the lumen of endoplasmic reticulum are performed by host signal peptidase, wereas cleavages in the cytoplasmic side are performed by serine protease NS3. Signal cleavage at the 2K-4B site requires a prior NS3 protease-mediated cleavage at the 4A-2K site.
Non-structural protein 1: N-glycosylated. The excreted form is glycosylated and this is required for efficient secretion of the protein from infected cells.
Keywords
- PTM
Interaction
Subunit
Envelope protein E
Homodimer; in the endoplasmic reticulum and Golgi. Interacts with protein prM. Interacts with non-structural protein 1.
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 98-111 | Fusion peptide | ||||
Sequence: DRGWGNGCGLFGKG |
Domain
The transmembrane domains of the small envelope protein M and envelope protein E contain an endoplasmic reticulum retention signal.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusFragment
- Length495
- Mass (Da)54,326
- Last updated1990-01-01 v1
- Checksum02FCBACC8CB6721E
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: M | ||||||
Non-terminal residue | 495 | |||||
Sequence: A |