P13658 · MBEA_ECOLX
- ProteinDNA relaxase MbeA
- GenembeA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids517 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Relaxase involved in plasmid ColE1 conjugative mobilization and is thus essential to promote the specific transfer of the plasmid during conjugation. First catalyzes the specific cleavage of one of the DNA strands at oriT, forming a covalent 5'-phosphotyrosine intermediate. The nic site corresponds to 5'-1469CTGG/CTTA1462-3' in the cleaved strand. The cleaved strand is then transferred through the dedicated type IV secretion apparatus. MbeA remains covalently linked at the 5' end of the strand, and once in the recipient cell, it probably catalyzes the rejoining of the two ends of the strand, re-forming the circular plasmid DNA. Is functional in vitro without a requirement for the conjugative accessory proteins.
Miscellaneous
The 100 C-terminal amino acids are dispensable for activity.
Catalytic activity
Cofactor
Co2+ (UniProtKB | Rhea| CHEBI:48828 )
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )
Note: Divalent metal cation. Can use Mg2+, Co2+ or Ni2+ with similar efficiencies, but is not active in the presence of Mn2+.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 19 | O-(5'-phospho-DNA)-tyrosine intermediate | |||
Binding site | 97 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 104 | a divalent metal cation (UniProtKB | ChEBI) | |||
Binding site | 106 | a divalent metal cation (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type I (single strand cut, ATP-independent) activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA relaxase MbeA
- EC number
- Alternative names
Gene names
Encoded on
- Plasmid ColE1
Organism names
- Organism
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP13658
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 19 | Loss of in vitro DNA-cleavage activity and in vivo functionality. | |||
Mutagenesis | 97 | Loss of in vitro DNA-cleavage activity and in vivo functionality. No effect on DNA binding around the nic site. | |||
Mutagenesis | 104 | Loss of in vitro DNA-cleavage activity and in vivo functionality. No effect on DNA binding around the nic site. | |||
Mutagenesis | 104 | Loss of the in vitro DNA-cleavage and strand-transfer activities but displays 1% of the in vivo wild-type activity; when associated with H-106. | |||
Mutagenesis | 106 | 4-fold and 3-fold reduction in the in vitro DNA-cleavage and strand-transfer activities, respectively, but no reduction in the in vivo functionality. | |||
Mutagenesis | 106 | Loss of the in vitro DNA-cleavage and strand-transfer activities but displays 1% of the in vivo wild-type activity; when associated with H-104. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000068400 | 1-517 | DNA relaxase MbeA | ||
Interaction
Subunit
Interacts with MbeB and MbeC to form the relaxosome.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | P13658 | mbeC P13657 | 2 | EBI-7798346, EBI-7798318 |
Protein-protein interaction databases
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length517
- Mass (Da)57,808
- Last updated1990-01-01 v1
- Checksum278E41B2D3B2A08A
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 294-310 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X15873 EMBL· GenBank· DDBJ | CAA33883.1 EMBL· GenBank· DDBJ | Genomic DNA |