P13574 · STE12_YEAST
- ProteinProtein STE12
- GeneSTE12
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids688 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Binds to the DNA sequence mediating pheromone induction (called the pheromone response element = PRE) which is found in the upstream control region of several a-, alpha- and haploid-specific genes. Involved in mating of haploids and in pseudohyphae formation in diploids.
Miscellaneous
Present with 1920 molecules/cell in log phase SD medium.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 57-167 | |||||
Sequence: NQIIRRYYLNSGQGFVSCVFWNNLYYITGTDIVKCCLYRMQKFGREVVQKKKFEEGIFSDLRNLKCGIDATLEQPKSEFLSFLFRNMCLKTQKKQKVFFWFSVAHDKLFAD |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Cellular Component | Ste12p-Dig1p-Dig2p complex | |
Cellular Component | Tec1p-Ste12p-Dig1p complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Biological Process | cellular response to pheromone | |
Biological Process | conjugation with cellular fusion | |
Biological Process | invasive growth in response to glucose limitation | |
Biological Process | negative regulation of mating-type specific transcription, DNA-templated | |
Biological Process | negative regulation of transcription by RNA polymerase II | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | pseudohyphal growth | |
Biological Process | regulation of filamentous growth | |
Biological Process | regulation of pseudohyphal growth | |
Biological Process | sexual reproduction |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein STE12
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP13574
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000072262 | 1-688 | Protein STE12 | |||
Sequence: MKVQITNSRTEEILKVQANNENDEVSKATPGEVEESLRLIGDLKFFLATAPVNWQENQIIRRYYLNSGQGFVSCVFWNNLYYITGTDIVKCCLYRMQKFGREVVQKKKFEEGIFSDLRNLKCGIDATLEQPKSEFLSFLFRNMCLKTQKKQKVFFWFSVAHDKLFADALERDLKRESLNQPSTTKPVNEPALSFSYDSSSDKPLYDQLLQHLDSRRPSSTTKSDNSPPKLESENFKDNELVTVTNQPLLGVGLMDDDAPESPSQINDFIPQKLIIEPNTLELNGLTEETPHDLPKNTAKGRDEEDFPLDYFPVSVEYPTEENAFDPFPPQAFTPAAPSMPISYDNVNERDSMPVNSLLNRYPYQLSVAPTFPVPPSSSRQHFMTNRDFYSSNNNKEKLVSPSDPTSYMKYDEPVMDFDESRPNENCTNAKSHNSGQQTKQHQLYSNNFQQSYPNGMVPGYYPKMPYNPMGGDPLLDQAFYGADDFFFPPEGCDNNMLYPQTATSWNVLPPQAMQPAPTYVGRPYTPNYRSTPGSAMFPYMQSSNSMQWNTAVSPYSSRAPSTTAKNYPPSTFYSQNINQYPRRRTVGMKSSQGNVPTGNKQSVGKSAKISKPLHIKTSAYQKQYKINLETKARPSAGDEDSAHPDKNKEISMPTPDSNTLVVQSEEGGAHSLEVDTNRRSDKNLPDAT | ||||||
Modified residue | 29 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 214 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 226 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 289 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 400 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Phosphorylated by the STE7, STE11 and STE20 kinases.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with mating-type protein ALPHA1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P13574 | DIG1 Q03063 | 7 | EBI-18264, EBI-29752 | |
BINARY | P13574 | DIG2 Q03373 | 10 | EBI-18264, EBI-34019 | |
BINARY | P13574 | KSS1 P14681 | 8 | EBI-18264, EBI-9945 | |
BINARY | P13574 | MATALPHA1 P0CY06 | 2 | EBI-18264, EBI-10438 | |
BINARY | P13574 | SLT2 Q00772 | 2 | EBI-18264, EBI-17372 | |
BINARY | P13574 | TEC1 P18412 | 13 | EBI-18264, EBI-19091 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 177-199 | Disordered | ||||
Sequence: SLNQPSTTKPVNEPALSFSYDSS | ||||||
Compositional bias | 179-199 | Polar residues | ||||
Sequence: NQPSTTKPVNEPALSFSYDSS | ||||||
Region | 211-239 | Disordered | ||||
Sequence: HLDSRRPSSTTKSDNSPPKLESENFKDNE | ||||||
Compositional bias | 212-228 | Polar residues | ||||
Sequence: LDSRRPSSTTKSDNSPP | ||||||
Region | 285-305 | Disordered | ||||
Sequence: LTEETPHDLPKNTAKGRDEED | ||||||
Compositional bias | 587-606 | Polar residues | ||||
Sequence: GMKSSQGNVPTGNKQSVGKS | ||||||
Region | 587-610 | Disordered | ||||
Sequence: GMKSSQGNVPTGNKQSVGKSAKIS | ||||||
Region | 630-688 | Disordered | ||||
Sequence: TKARPSAGDEDSAHPDKNKEISMPTPDSNTLVVQSEEGGAHSLEVDTNRRSDKNLPDAT | ||||||
Compositional bias | 632-650 | Basic and acidic residues | ||||
Sequence: ARPSAGDEDSAHPDKNKEI | ||||||
Compositional bias | 651-665 | Polar residues | ||||
Sequence: SMPTPDSNTLVVQSE | ||||||
Compositional bias | 671-688 | Basic and acidic residues | ||||
Sequence: SLEVDTNRRSDKNLPDAT |
Domain
The DNA-binding domain seems to be involved in the suppression of pseudohyphae formation under nitrogen-rich conditions and in haploids. This region is also involved in the regulation of budding pattern of haploids.
Sequence similarities
Belongs to the STE12 transcription factor family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length688
- Mass (Da)77,867
- Last updated1990-01-01 v1
- ChecksumEE7371F18BBC0337
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 179-199 | Polar residues | ||||
Sequence: NQPSTTKPVNEPALSFSYDSS | ||||||
Compositional bias | 212-228 | Polar residues | ||||
Sequence: LDSRRPSSTTKSDNSPP | ||||||
Compositional bias | 587-606 | Polar residues | ||||
Sequence: GMKSSQGNVPTGNKQSVGKS | ||||||
Compositional bias | 632-650 | Basic and acidic residues | ||||
Sequence: ARPSAGDEDSAHPDKNKEI | ||||||
Compositional bias | 651-665 | Polar residues | ||||
Sequence: SMPTPDSNTLVVQSE | ||||||
Compositional bias | 671-688 | Basic and acidic residues | ||||
Sequence: SLEVDTNRRSDKNLPDAT |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X16112 EMBL· GenBank· DDBJ | CAA34246.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M24502 EMBL· GenBank· DDBJ | AAA35109.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U10556 EMBL· GenBank· DDBJ | AAB68884.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006934 EMBL· GenBank· DDBJ | DAA06779.1 EMBL· GenBank· DDBJ | Genomic DNA |