P13528 · UNC86_CAEEL

  • Protein
    Transcription factor unc-86
  • Gene
    unc-86
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Transcription factor required for correct cell fate determination and differentiation in diverse neuronal cell lineages where it plays a role in specifying the fate of daughter cells during cell divisions (PubMed:10899123, PubMed:2257628, PubMed:26096732, PubMed:7237544).
Involved in sensory neuron production and function (PubMed:10899123, PubMed:1361171, PubMed:26096732, PubMed:9735371).
Binds both alone and with mec-3 to the mec-3 promoter to initiate and maintain mec-3 expression which is required for sensory neuron differentiation (PubMed:1361171, PubMed:9735371).
In addition, binds both alone and with mec-3 to the promoters of mec-4 and mec-7 which act to regulate sensory neuron function (PubMed:10899123, PubMed:9735371).
Involved in determining the identity of the serotonergic NSM neurons and the cholinergic IL2 sensory and URA motor neurons (PubMed:24353061).
Promotes expression of the cfi-1 transcription factor in the URA and IL2 neurons which in turn activates normal URA and IL2 gene expression (PubMed:11959845, PubMed:24353061).
Required to determine the identity of BDU sensory neurons in concert with transcription factor unc-86, regulating expression of a number of genes, including transcription factors ceh-14 and ahr-1, neuropeptides flp-10, nlp-1 and nlp-15, and tyramine receptor-encoding ser-2 (PubMed:26096732).
Regulates expression of a number of genes in NSM neurons including bas-1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1 (PubMed:24353061).
In the IL2 neurons, required for expression of cho-1, gcy-19, klp-6, lag-2, unc-5 and unc-17 (PubMed:24353061).
Promotes expression of pkd-2 in the male-specific CEM head neurons (PubMed:11959845).
Required for dauer-specific branching of IL2Q neurons and nictation behavior (PubMed:23932402).
Controls both the timing and direction of axon outgrowth in HSN neurons (PubMed:21656875).
Plays a role in serotonin production by regulating expression of the tryptophan hydrolase tph-1 which catalyzes serotonin synthesis, in the AIM, NSM, HSN and RIH neurons (PubMed:12135927).
Involved in regulation of lin-11 expression in the AIZ interneurons, the major interneurons of the olfactory pathway, and is required for odortaxis behavior (PubMed:12883006).
Involved in neurite pruning between AIM neurons during larval development by regulating the expression of transcription factor mbr-1 (PubMed:16139210).
Required for correct localization of unc-40 (PubMed:24353061).

Features

Showing features for dna binding.

TypeIDPosition(s)Description
DNA binding253-312Homeobox

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular ComponentRNA polymerase II transcription regulator complex
Molecular FunctionDNA-binding transcription activator activity, RNA polymerase II-specific
Molecular FunctionDNA-binding transcription factor activity, RNA polymerase II-specific
Molecular FunctionRNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular FunctionRNA polymerase II transcription regulatory region sequence-specific DNA binding
Molecular FunctionRNA polymerase II-specific DNA-binding transcription factor binding
Molecular Functionsequence-specific DNA binding
Biological Processmechanosensory behavior
Biological Processneuron development
Biological Processneuron fate specification
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processregulation of transcription by RNA polymerase II
Biological Processresponse to mechanical stimulus
Biological Processsensory perception of smell

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Transcription factor unc-86
  • Alternative names
    • Uncoordinated protein 86

Gene names

    • Name
      unc-86
    • ORF names
      C30A5.7

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    P13528
  • Secondary accessions
    • D7SFI2

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

In a number of neuronal cell lineages, one of the two daughter cells of a division fails to assume its normal fate, retaining instead the fate of its parent cell. Altered gene expression in a number of neuron types including reduced cat-1 expression in AIM, NSM, HSN and RIH neurons, loss of cat-4 expression in HSN neurons, loss of expression of dop-3 and mgl-3 and reduced expression of bas-1, nlp-13, ptpa-1 and scd-2 in NSM neurons, loss of expression of gcy-19, klp-6, lag-2 and unc-17 and reduced expression of cho-1 and unc-5 in IL2 neurons, and loss of tph-1 expression in AIM, NSM, HSN and RIH neurons which leads to defects in serotonin production. Defective motility along with defects in IL2Q dendritic arborization and nictation. Abnormal neurite outgrowth in NSM neurons. Defective response to odor attractants but odor repellants are avoided normally. Penetrant ventral axon growth defects in HSN motor neurons with extension of processes in L1, three stages earlier than wild-type. Incorrect unc-40 localization. Defective egg-laying characterized by retention of substantially more eggs in the uterus of second-day animals than the wild-type.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis160In u168; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 gene expression. No egg-laying or Q neuroblast lineage defects.
Mutagenesis210In u5; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 gene expression. No egg-laying or Q neuroblast lineage defects. Abolishes expression of ALM neuron-specific genes mec-17 and mec-4. Abnormal, ectopic expression of BDU-specific genes ceh-14, flp-10 and zig-3 in ALM.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001007801-357Transcription factor unc-86

Proteomic databases

Expression

Tissue specificity

Specific to neurons and neuroblasts. Expressed in CEM head neurons and in IL2, URA, URB, URX and URY neurons. Not expressed in olfactory sensory neurons but expressed in AIZ interneurons.

Developmental stage

First appears during embryogenesis and persists throughout adulthood.

Gene expression databases

    • WBGene00006818Expressed in pharyngeal muscle cell (C elegans) and 3 other cell types or tissues

Interaction

Subunit

Interacts with mec-3; the heterooligomer binds to the promoters of mec-3, mec-4 and mec-7.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif, domain.

TypeIDPosition(s)Description
Motif35-44POU-IV box
Domain155-232POU-specific

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

P13528-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    357
  • Mass (Da)
    39,737
  • Last updated
    2022-10-12 v2
  • Checksum
    1B9830CA8E04ADAC
MQNTAPVPTTTTASKMQPFNNSLFGSFDDPILNARAAQVALADIDVKNVPQLTNPLMRPHDMFSYSNYFSGIHDTSAATNIYQGLPSSSEPFDASVVVPTSSDDQMTPLQQVMAMQQSYGAPPPFQYNMTHPFSTTSIASSNNLARYPIAPPTSDMDTDPRQLETFAEHFKQRRIKLGVTQADVGKALAHLKMPGVGSLSQSTICRFESLTLSHNNMVALKPILHSWLEKAEEAMKQKDTIGDINGILPNTDKKRKRTSIAAPEKRELEQFFKQQPRPSGERIASIADRLDLKKNVVRVWFCNQRQKQKRDFRSQFRARSAAAVMGPRVMPVLNGNNSNNNLKQGQTTYNGLPGFFD

P13528-2

  • Name
    a
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence AAA28158.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence AAA28159.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0023441-56in isoform a

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M22363
EMBL· GenBank· DDBJ
AAA28158.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
M22363
EMBL· GenBank· DDBJ
AAA28159.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
BX284603
EMBL· GenBank· DDBJ
CCD62655.2
EMBL· GenBank· DDBJ
Genomic DNA
BX284603
EMBL· GenBank· DDBJ
CCD62656.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp