P13528 · UNC86_CAEEL
- ProteinTranscription factor unc-86
- Geneunc-86
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids357 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription factor required for correct cell fate determination and differentiation in diverse neuronal cell lineages where it plays a role in specifying the fate of daughter cells during cell divisions (PubMed:10899123, PubMed:2257628, PubMed:26096732, PubMed:7237544).
Involved in sensory neuron production and function (PubMed:10899123, PubMed:1361171, PubMed:26096732, PubMed:9735371).
Binds both alone and with mec-3 to the mec-3 promoter to initiate and maintain mec-3 expression which is required for sensory neuron differentiation (PubMed:1361171, PubMed:9735371).
In addition, binds both alone and with mec-3 to the promoters of mec-4 and mec-7 which act to regulate sensory neuron function (PubMed:10899123, PubMed:9735371).
Involved in determining the identity of the serotonergic NSM neurons and the cholinergic IL2 sensory and URA motor neurons (PubMed:24353061).
Promotes expression of the cfi-1 transcription factor in the URA and IL2 neurons which in turn activates normal URA and IL2 gene expression (PubMed:11959845, PubMed:24353061).
Required to determine the identity of BDU sensory neurons in concert with transcription factor unc-86, regulating expression of a number of genes, including transcription factors ceh-14 and ahr-1, neuropeptides flp-10, nlp-1 and nlp-15, and tyramine receptor-encoding ser-2 (PubMed:26096732).
Regulates expression of a number of genes in NSM neurons including bas-1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1 (PubMed:24353061).
In the IL2 neurons, required for expression of cho-1, gcy-19, klp-6, lag-2, unc-5 and unc-17 (PubMed:24353061).
Promotes expression of pkd-2 in the male-specific CEM head neurons (PubMed:11959845).
Required for dauer-specific branching of IL2Q neurons and nictation behavior (PubMed:23932402).
Controls both the timing and direction of axon outgrowth in HSN neurons (PubMed:21656875).
Plays a role in serotonin production by regulating expression of the tryptophan hydrolase tph-1 which catalyzes serotonin synthesis, in the AIM, NSM, HSN and RIH neurons (PubMed:12135927).
Involved in regulation of lin-11 expression in the AIZ interneurons, the major interneurons of the olfactory pathway, and is required for odortaxis behavior (PubMed:12883006).
Involved in neurite pruning between AIM neurons during larval development by regulating the expression of transcription factor mbr-1 (PubMed:16139210).
Required for correct localization of unc-40 (PubMed:24353061).
Involved in sensory neuron production and function (PubMed:10899123, PubMed:1361171, PubMed:26096732, PubMed:9735371).
Binds both alone and with mec-3 to the mec-3 promoter to initiate and maintain mec-3 expression which is required for sensory neuron differentiation (PubMed:1361171, PubMed:9735371).
In addition, binds both alone and with mec-3 to the promoters of mec-4 and mec-7 which act to regulate sensory neuron function (PubMed:10899123, PubMed:9735371).
Involved in determining the identity of the serotonergic NSM neurons and the cholinergic IL2 sensory and URA motor neurons (PubMed:24353061).
Promotes expression of the cfi-1 transcription factor in the URA and IL2 neurons which in turn activates normal URA and IL2 gene expression (PubMed:11959845, PubMed:24353061).
Required to determine the identity of BDU sensory neurons in concert with transcription factor unc-86, regulating expression of a number of genes, including transcription factors ceh-14 and ahr-1, neuropeptides flp-10, nlp-1 and nlp-15, and tyramine receptor-encoding ser-2 (PubMed:26096732).
Regulates expression of a number of genes in NSM neurons including bas-1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1 (PubMed:24353061).
In the IL2 neurons, required for expression of cho-1, gcy-19, klp-6, lag-2, unc-5 and unc-17 (PubMed:24353061).
Promotes expression of pkd-2 in the male-specific CEM head neurons (PubMed:11959845).
Required for dauer-specific branching of IL2Q neurons and nictation behavior (PubMed:23932402).
Controls both the timing and direction of axon outgrowth in HSN neurons (PubMed:21656875).
Plays a role in serotonin production by regulating expression of the tryptophan hydrolase tph-1 which catalyzes serotonin synthesis, in the AIM, NSM, HSN and RIH neurons (PubMed:12135927).
Involved in regulation of lin-11 expression in the AIZ interneurons, the major interneurons of the olfactory pathway, and is required for odortaxis behavior (PubMed:12883006).
Involved in neurite pruning between AIM neurons during larval development by regulating the expression of transcription factor mbr-1 (PubMed:16139210).
Required for correct localization of unc-40 (PubMed:24353061).
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 253-312 | Homeobox | ||||
Sequence: KKRKRTSIAAPEKRELEQFFKQQPRPSGERIASIADRLDLKKNVVRVWFCNQRQKQKRDF |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | RNA polymerase II transcription regulator complex | |
Molecular Function | DNA-binding transcription activator activity, RNA polymerase II-specific | |
Molecular Function | DNA-binding transcription factor activity, RNA polymerase II-specific | |
Molecular Function | RNA polymerase II cis-regulatory region sequence-specific DNA binding | |
Molecular Function | RNA polymerase II transcription regulatory region sequence-specific DNA binding | |
Molecular Function | RNA polymerase II-specific DNA-binding transcription factor binding | |
Molecular Function | sequence-specific DNA binding | |
Biological Process | mechanosensory behavior | |
Biological Process | neuron development | |
Biological Process | neuron fate specification | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | regulation of transcription by RNA polymerase II | |
Biological Process | response to mechanical stimulus | |
Biological Process | sensory perception of smell |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranscription factor unc-86
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionP13528
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
In a number of neuronal cell lineages, one of the two daughter cells of a division fails to assume its normal fate, retaining instead the fate of its parent cell. Altered gene expression in a number of neuron types including reduced cat-1 expression in AIM, NSM, HSN and RIH neurons, loss of cat-4 expression in HSN neurons, loss of expression of dop-3 and mgl-3 and reduced expression of bas-1, nlp-13, ptpa-1 and scd-2 in NSM neurons, loss of expression of gcy-19, klp-6, lag-2 and unc-17 and reduced expression of cho-1 and unc-5 in IL2 neurons, and loss of tph-1 expression in AIM, NSM, HSN and RIH neurons which leads to defects in serotonin production. Defective motility along with defects in IL2Q dendritic arborization and nictation. Abnormal neurite outgrowth in NSM neurons. Defective response to odor attractants but odor repellants are avoided normally. Penetrant ventral axon growth defects in HSN motor neurons with extension of processes in L1, three stages earlier than wild-type. Incorrect unc-40 localization. Defective egg-laying characterized by retention of substantially more eggs in the uterus of second-day animals than the wild-type.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 160 | In u168; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 gene expression. No egg-laying or Q neuroblast lineage defects. | ||||
Sequence: P → S | ||||||
Mutagenesis | 210 | In u5; defective mechanosensation, lack of interaction with mec-3 and lack of maintained mec-3 gene expression. No egg-laying or Q neuroblast lineage defects. Abolishes expression of ALM neuron-specific genes mec-17 and mec-4. Abnormal, ectopic expression of BDU-specific genes ceh-14, flp-10 and zig-3 in ALM. | ||||
Sequence: L → F |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000100780 | 1-357 | Transcription factor unc-86 | |||
Sequence: MQNTAPVPTTTTASKMQPFNNSLFGSFDDPILNARAAQVALADIDVKNVPQLTNPLMRPHDMFSYSNYFSGIHDTSAATNIYQGLPSSSEPFDASVVVPTSSDDQMTPLQQVMAMQQSYGAPPPFQYNMTHPFSTTSIASSNNLARYPIAPPTSDMDTDPRQLETFAEHFKQRRIKLGVTQADVGKALAHLKMPGVGSLSQSTICRFESLTLSHNNMVALKPILHSWLEKAEEAMKQKDTIGDINGILPNTDKKRKRTSIAAPEKRELEQFFKQQPRPSGERIASIADRLDLKKNVVRVWFCNQRQKQKRDFRSQFRARSAAAVMGPRVMPVLNGNNSNNNLKQGQTTYNGLPGFFD |
Proteomic databases
Expression
Tissue specificity
Specific to neurons and neuroblasts. Expressed in CEM head neurons and in IL2, URA, URB, URX and URY neurons. Not expressed in olfactory sensory neurons but expressed in AIZ interneurons.
Developmental stage
First appears during embryogenesis and persists throughout adulthood.
Gene expression databases
Structure
Family & Domains
Features
Showing features for motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 35-44 | POU-IV box | ||||
Sequence: RAAQVALADI | ||||||
Domain | 155-232 | POU-specific | ||||
Sequence: DMDTDPRQLETFAEHFKQRRIKLGVTQADVGKALAHLKMPGVGSLSQSTICRFESLTLSHNNMVALKPILHSWLEKAE |
Sequence similarities
Belongs to the POU transcription factor family. Class-4 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
P13528-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Nameb
- Length357
- Mass (Da)39,737
- Last updated2022-10-12 v2
- Checksum1B9830CA8E04ADAC
P13528-2
- Namea
- Differences from canonical
- 1-56: Missing
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_002344 | 1-56 | in isoform a | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M22363 EMBL· GenBank· DDBJ | AAA28158.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
M22363 EMBL· GenBank· DDBJ | AAA28159.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BX284603 EMBL· GenBank· DDBJ | CCD62655.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284603 EMBL· GenBank· DDBJ | CCD62656.1 EMBL· GenBank· DDBJ | Genomic DNA |