P13498 · CY24A_HUMAN

  • Protein
    Cytochrome b-245 light chain
  • Gene
    CYBA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Features

Showing features for binding site.

119520406080100120140160180
TypeIDPosition(s)Description
Binding site94Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentendosome
Cellular Componentmembrane
Cellular ComponentNADPH oxidase complex
Cellular Componentphagocytic vesicle membrane
Cellular Componentplasma membrane
Cellular Componentsecretory granule
Cellular Componentspecific granule membrane
Cellular Componenttertiary granule membrane
Molecular Functionelectron transfer activity
Molecular Functionheme binding
Molecular Functionmetal ion binding
Molecular Functionoxidoreductase activity
Molecular Functionprotein heterodimerization activity
Molecular FunctionSH3 domain binding
Biological Processcytochrome complex assembly
Biological Processestablishment of localization in cell
Biological Processhydrogen peroxide biosynthetic process
Biological Processinflammatory response
Biological Processinnate immune response
Biological Processmucus secretion
Biological Processpositive regulation of defense response to bacterium
Biological Processpositive regulation of interleukin-6 production
Biological Processpositive regulation of mucus secretion
Biological Processpositive regulation of phagocytosis
Biological Processpositive regulation of reactive oxygen species biosynthetic process
Biological Processpositive regulation of toll-like receptor 2 signaling pathway
Biological Processpositive regulation of tumor necrosis factor production
Biological Processregulation of release of sequestered calcium ion into cytosol
Biological Processrespiratory burst
Biological Processsmooth muscle hypertrophy
Biological Processsuperoxide anion generation
Biological Processsuperoxide metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome b-245 light chain
  • Alternative names
    • Cytochrome b(558) alpha chain
    • Cytochrome b558 subunit alpha
    • Neutrophil cytochrome b 22 kDa polypeptide
    • Superoxide-generating NADPH oxidase light chain subunit
    • p22 phagocyte B-cytochrome
    • p22-phox (p22phox)

Gene names

    • Name
      CYBA

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo

Accessions

  • Primary accession
    P13498
  • Secondary accessions
    • Q14090
    • Q9BR72

Proteomes

Organism-specific databases

Subcellular Location

Features

Showing features for intramembrane.

TypeIDPosition(s)Description
Intramembrane91-127

Keywords

Disease & Variants

Involvement in disease

Granulomatous disease, chronic, autosomal recessive, 4 (CGD4)

  • Note
    • The disease is caused by variants affecting the gene represented in this entry
  • Description
    A form of chronic granulomatous disease, a primary immunodeficiency characterized by severe recurrent bacterial and fungal infections, along with manifestations of chronic granulomatous inflammation. It results from an impaired ability of phagocytes to mount a burst of reactive oxygen species in response to pathogens.
  • See also
    MIM:233690
Natural variants in CGD4
Variant IDPosition(s)ChangeDescription
VAR_01275524G>Rin CGD4; dbSNP:rs28941476
VAR_07186025G>Din CGD4; dbSNP:rs179363891
VAR_06057625G>Vin CGD4; dbSNP:rs179363891
VAR_06057752L>Pin CGD4; dbSNP:rs179363890
VAR_06057853E>Vin CGD4; dbSNP:rs179363893
VAR_00512390R>Qin CGD4; dbSNP:rs104894513
VAR_06057990R>Win CGD4; dbSNP:rs179363892
VAR_00512494H>Rin CGD4; dbSNP:rs104894510
VAR_005125118S>Rin CGD4; dbSNP:rs104894514
VAR_060580124A>Vin CGD4; dbSNP:rs179363894
VAR_060581125A>Tin CGD4; dbSNP:rs119103269
VAR_005126156P>Qin CGD4; dbSNP:rs104894515

Features

Showing features for natural variant, mutagenesis.

TypeIDPosition(s)Description
Natural variantVAR_01275524in CGD4; dbSNP:rs28941476
Natural variantVAR_07186025in CGD4; dbSNP:rs179363891
Natural variantVAR_06057625in CGD4; dbSNP:rs179363891
Natural variantVAR_06057752in CGD4; dbSNP:rs179363890
Natural variantVAR_06057853in CGD4; dbSNP:rs179363893
Natural variantVAR_00512272in dbSNP:rs4673
Natural variantVAR_00512390in CGD4; dbSNP:rs104894513
Natural variantVAR_06057990in CGD4; dbSNP:rs179363892
Natural variantVAR_00512494in CGD4; dbSNP:rs104894510
Natural variantVAR_005125118in CGD4; dbSNP:rs104894514
Natural variantVAR_060580124in CGD4; dbSNP:rs179363894
Natural variantVAR_060581125in CGD4; dbSNP:rs119103269
Natural variantVAR_005126156in CGD4; dbSNP:rs104894515
Mutagenesis157Loss of interaction with NOXO1.
Natural variantVAR_060582171in dbSNP:rs72667005
Natural variantVAR_054801174in dbSNP:rs1049254
Natural variantVAR_060583193in dbSNP:rs72667006

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 343 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Keywords

Organism-specific databases

Miscellaneous

Chemistry

Genetic variation databases

PTM/Processing

Features

Showing features for initiator methionine, chain, modified residue, modified residue (large scale data), cross-link.

TypeIDPosition(s)SourceDescription
Initiator methionine1UniProtRemoved
ChainPRO_00001449072-195UniProtCytochrome b-245 light chain
Modified residue147UniProtPhosphothreonine
Modified residue (large scale data)147PRIDEPhosphothreonine
Cross-link149UniProtGlycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Modified residue (large scale data)153PRIDEPhosphoserine
Modified residue168UniProtPhosphoserine
Modified residue (large scale data)168PRIDEPhosphoserine

Post-translational modification

The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.
Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.
Ubiquitinated at Lys-149 likely by RNF145.

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Organism-specific databases

Interaction

Subunit

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity).
Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX4 (PubMed:15927447).
Interacts with calprotectin (S100A8/9) (PubMed:22808130).
Interacts with GBP7 (By similarity).
Interacts with NOXO1 (PubMed:17140397).
Forms a heterodimer with NOX3 and is essential for activity and cell membrane localization of NOX3 (PubMed:15824103, PubMed:17140397).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P13498NCF1 P145987EBI-986058, EBI-395044
BINARY P13498NOXO1 Q8NFA24EBI-986058, EBI-7130806
BINARY P13498NOXO1 Q8NFA2-33EBI-986058, EBI-20557410
View interactors in UniProtKB
View CPX-1017 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region134-195Disordered

Sequence similarities

Belongs to the p22phox family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    195
  • Mass (Da)
    21,013
  • Last updated
    2010-11-02 v3
  • Checksum
    428427AD19398240
MGQIEWAMWANEQALASGLILITGGIVATAGRFTQWYFGAYSIVAGVFVCLLEYPRGKRKKGSTMERWGQKYMTAVVKLFGPFTRNYYVRAVLHLLLSVPAGFLLATILGTACLAIASGIYLLAAVRGEQWTPIEPKPRERPQIGGTIKQPPSNPPPRPPAEARKKPSEEEAAVAAGGPPGGPQVNPIPVTDEVV

Computationally mapped potential isoform sequences

There are 13 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
H3BT77H3BT77_HUMANCYBA130
H3BR52H3BR52_HUMANCYBA128
H3BPZ7H3BPZ7_HUMANCYBA145
H3BPX1H3BPX1_HUMANCYBA145
H3BNP7H3BNP7_HUMANCYBA210
A0A8Q3WLM1A0A8Q3WLM1_HUMANCYBA176
A0A8Q3WL14A0A8Q3WL14_HUMANCYBA178
A0A8Q3WL20A0A8Q3WL20_HUMANCYBA204
A0A8Q3WL26A0A8Q3WL26_HUMANCYBA254
A0A8Q3WL27A0A8Q3WL27_HUMANCYBA175
A0A8Q3SIH4A0A8Q3SIH4_HUMANCYBA167
A0A8Q3SIJ9A0A8Q3SIJ9_HUMANCYBA129
A0A8Q3SJ80A0A8Q3SJ80_HUMANCYBA153

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M21186
EMBL· GenBank· DDBJ
AAA90925.1
EMBL· GenBank· DDBJ
mRNA
BT006861
EMBL· GenBank· DDBJ
AAP35507.1
EMBL· GenBank· DDBJ
mRNA
AC116552
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
BC006465
EMBL· GenBank· DDBJ
AAH06465.1
EMBL· GenBank· DDBJ
mRNA
AH002664
EMBL· GenBank· DDBJ
AAA52134.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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