P13288 · KR2_EBVB9
- ProteinSerine/threonine-protein kinase BGLF4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids429 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays many key roles by phosphorylating several proteins including the viral DNA processivity factor BMRF1, EBNA1 or EBNA2. Modifies the host nuclear envelope structure and induces the redistribution of nuclear envelope-associated proteins by phosphorylating host nucleoporins. Subsequently, promotes the nuclear transport of EBV lytic proteins. Required for efficient lytic DNA replication and release of nucleocapsids from the nucleus. Contributes to the compaction of host cell chromatin in cells undergoing lytic replication, presumably by phosphorylating the host condensin complex and host TOP2A. Induces disassembly of the nuclear lamina by phosphorylating with host LMNA. Phosphorylates substrates involved in capsid assembly and DNA packaging. Facilitates the switch from latent to lytic DNA replication by down-regulating EBNA1 replication function. Phosphorylates the viral immediate-early protein BZLF1 and inhibits its sumoylation by interacting with host SUMO1 and SUMO2. Phosphorylates also host SAMHD1 and thereby counteracts its antiviral effect by reducing its dNTP hydrolase activity.
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nuclear envelope | |
Cellular Component | host cell nucleus | |
Cellular Component | viral tegument | |
Molecular Function | ATP binding | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | modulation by virus of host chromatin organization | |
Biological Process | symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
BGLF4 plays an important role in post-replication events that are necessary for expression of late genes.
Names & Taxonomy
Protein names
- Recommended nameSerine/threonine-protein kinase BGLF4
- EC number
Gene names
- Community suggested namesBGLF4
Organism names
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Peploviricota > Herviviricetes > Herpesvirales > Orthoherpesviridae > Gammaherpesvirinae > Lymphocryptovirus > Lymphocryptovirus humangamma4 > Epstein-Barr virus (strain GD1)
- Virus hosts
Accessions
- Primary accessionP13288
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: the protein is present at discrete sites in nuclei, called replication compartments where viral DNA replication occurs.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000086184 | 1-429 | Serine/threonine-protein kinase BGLF4 | |||
Sequence: MDVNMAAELSPTNSSSSGELSVSPEPPRETQAFLGKVTVIDYFTFQHKHLKVTNIDDMTETLYVKLPENMTRCDHLPITCEYLLGRGSYGAVYAHADNATVKLYDSVTELYHELMVCDMIQIGKATAEDGQDKALVDYLSACTSCHALFMPQFRCSLQDYGHWHDGSIEPLVRGFQGLKDAVYFLNRHCGLFHSDISPSNILVDFTDTMWGMGRLVLTDYGTASLHDRNKMLDVRLKSSKGRQLYRLYCQREPFSIAKDTYKPLCLLSKCYILRGAGHIPDPSACGPVGAQTALRLDLQSLGYSLLYGIMHLADSTHKIPYPNPDMGFDRSDPLYFLQFAAPKVVLLEVLSQMWNLNLDMGLTSCGESPCVDVTAEHMSQFLQWCRSLKKRFKESYFFNCRPRFEHPHLPGLVAELLADDFFGPDGRRG |
PTM databases
Expression
Keywords
- Developmental stage
Interaction
Subunit
Interacts with host NUP62 and NUP153; this interaction plays a role in nuclear targeting of BGLF4. Interacts with host SUMO1 and SUMO2.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | P13288 | XPC Q01831 | 9 | EBI-1630636, EBI-372610 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-27 | Disordered | ||||
Sequence: MDVNMAAELSPTNSSSSGELSVSPEPP | ||||||
Domain | 1-409 | Protein kinase | ||||
Sequence: MDVNMAAELSPTNSSSSGELSVSPEPPRETQAFLGKVTVIDYFTFQHKHLKVTNIDDMTETLYVKLPENMTRCDHLPITCEYLLGRGSYGAVYAHADNATVKLYDSVTELYHELMVCDMIQIGKATAEDGQDKALVDYLSACTSCHALFMPQFRCSLQDYGHWHDGSIEPLVRGFQGLKDAVYFLNRHCGLFHSDISPSNILVDFTDTMWGMGRLVLTDYGTASLHDRNKMLDVRLKSSKGRQLYRLYCQREPFSIAKDTYKPLCLLSKCYILRGAGHIPDPSACGPVGAQTALRLDLQSLGYSLLYGIMHLADSTHKIPYPNPDMGFDRSDPLYFLQFAAPKVVLLEVLSQMWNLNLDMGLTSCGESPCVDVTAEHMSQFLQWCRSLKKRFKESYFFNCRPRFEHPHL | ||||||
Compositional bias | 7-24 | Polar residues | ||||
Sequence: AELSPTNSSSSGELSVSP | ||||||
Region | 36-40 | SUMO interaction motif | ||||
Sequence: KVTVI | ||||||
Region | 344-350 | SUMO interaction motif | ||||
Sequence: VVLLEVL |
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length429
- Mass (Da)48,351
- Last updated2009-05-26 v2
- Checksum241ECF46F613A68B
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 7-24 | Polar residues | ||||
Sequence: AELSPTNSSSSGELSVSP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
V01555 EMBL· GenBank· DDBJ | CAA24828.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AJ507799 EMBL· GenBank· DDBJ | CAD53438.2 EMBL· GenBank· DDBJ | Genomic DNA |