P13203 · GLCDH_THEAC

Function

function

Catalyzes the NAD(P)+-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Is also significantly active with galactose as substrate, but not with mannose or glucose 6-phosphate. Can utilize both NAD+ and NADP+ as electron acceptor, with a marked preference for NADP+. Physiologically, may be involved in the degradation of both glucose and galactose through a non-phosphorylative variant of the Entner-Doudoroff pathway.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
10 mMglucose
0.113 mMNADP+
The KM value for NAD+ is superior to 30 mM, it could not be defined precisely as it was impossible to reach saturation of the enzyme.

Temperature Dependence

Thermostable. Retains full catalytic activity after 9 hours at 55 degrees Celsius, and at 75 degrees Celsius the half-life is approximately 3 hours.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site41Zn2+ (UniProtKB | ChEBI); catalytic
Binding site43substrate
Binding site68Zn2+ (UniProtKB | ChEBI); catalytic
Binding site69Zn2+ (UniProtKB | ChEBI); catalytic
Binding site119substrate
Binding site156substrate
Binding site156Zn2+ (UniProtKB | ChEBI); catalytic
Binding site160substrate
Binding site216-218NADP+ (UniProtKB | ChEBI)
Binding site275-277NADP+ (UniProtKB | ChEBI)
Binding site304-306NADP+ (UniProtKB | ChEBI)
Binding site306substrate
Binding site349NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionD-glucose binding
Molecular Functiongalactose 1-dehydrogenase (NADP+) activity
Molecular Functiongalactose 1-dehydrogenase activity
Molecular Functiongalactose binding
Molecular Functionglucose 1-dehydrogenase (NAD+) activity
Molecular Functionglucose 1-dehydrogenase (NADP+) activity
Molecular Functionglucose 1-dehydrogenase [NAD(P)+] activity
Molecular FunctionNAD+ binding
Molecular FunctionNADP+ binding
Molecular Functionzinc ion binding
Biological Processgalactose catabolic process via D-galactonate
Biological Processnon-phosphorylated glucose catabolic process
Biological Processprotein tetramerization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glucose 1-dehydrogenase
  • EC number
  • Short names
    GDH
    ; GlcDH
  • Alternative names
    • Galactose 1-dehydrogenase
      (EC:1.1.1.120
      , EC:1.1.1.48
      ) . EC:1.1.1.120 (UniProtKB | ENZYME | Rhea)
      , EC:1.1.1.48 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      gdh
    • Ordered locus names
      Ta0897

Organism names

Accessions

  • Primary accession
    P13203

Proteomes

Phenotypes & Variants

Features

Showing features for natural variant.

TypeIDPosition(s)Description
Natural variant2

Variants

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The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000798892-361Glucose 1-dehydrogenase

Proteomic databases

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    361
  • Mass (Da)
    40,250
  • Last updated
    2007-01-23 v4
  • Checksum
    4852179202F15208
MTEQKAIVTDAPKGGVKYTTIDMPEPEHYDAKLSPVYIGICGTDRGEVAGALSFTYNPEGENFLVLGHEALLRVDDARDNGYIKKGDLVVPLVRRPGKCINCRIGRQDNCSIGDPDKHEAGITGLHGFMRDVIYDDIEYLVKVEDPELGRIAVLTEPLKNVMKAFEVFDVVSKRSIFFGDDSTLIGKRMVIIGSGSEAFLYSFAGVDRGFDVTMVNRHDETENKLKIMDEFGVKFANYLKDMPEKIDLLVDTSGDPTTTFKFLRKVNNNGVVILFGTNGKAPGYPVDGEDIDYIVERNITIAGSVDAAKIHYVQALQSLSNWNRRHPDAMKSIITYEAKPSETNIFFQKPHGEIKTVIKWQ

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict338-361in Ref. 1; CAA42450

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X59788
EMBL· GenBank· DDBJ
CAA42450.1
EMBL· GenBank· DDBJ
Genomic DNA
AL445065
EMBL· GenBank· DDBJ
CAC12026.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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