P13202 · VIE1_HCMVA

Function

function

Plays an important role in transactivating viral early genes as well as activating its own promoter, probably by altering the viral chromatin structure (By similarity).
Expression of IE1 and IE2 proteins is critical for the establishment of lytic infection and reactivation from viral latency (PubMed:27466417).
Disrupts PML-associated ND10 nuclear bodies by interfering with host PML and SP100 sumoylation thereby altering the regulation of type I and type II interferon-induced gene expression (PubMed:27903803, PubMed:34370791).
Promotes efficient viral growth by interacting with and directing host SP100 to degradation, leading to enhanced acetylation level of histones (By similarity).
In addition, functions in counteracting the host innate antiviral response. Inhibits the type I interferon pathway by directly interacting with and sequestrating host STAT2 (By similarity).
Also targets type II interferon pathway by repressing IL6- and STAT3 target genes (By similarity).
Repression of STAT3 genes is due to STAT3 nuclear accumulation and disruption of IL6-induced STAT3 phosphorylation by IE1 (PubMed:23903834).
This repression is followed by phosphorylation and activation of STAT1 (By similarity).
Inhibits host ISG transcription by sequestering host ISGF3 in a PML- and STAT2- binding dependent manner (By similarity).
Alters host cell cycle progression, probably through its interaction with host E2F1 or RB1 that overcomes the RB1-mediated repression of E2F-responsive promoters (By similarity).

GO annotations

AspectTerm
Cellular Componenthost cell nucleus
Biological Processbidirectional double-stranded viral DNA replication
Biological ProcessDNA-templated viral transcription
Biological Processsymbiont-mediated perturbation of host cell cycle G1/S transition checkpoint
Biological Processsymbiont-mediated suppression of host innate immune response
Biological Processsymbiont-mediated suppression of host type I interferon-mediated signaling pathway
Biological Processvirus-mediated perturbation of host defense response

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Immediate early protein IE1
  • Short names
    IE1
  • Alternative names
    • 55 kDa immediate-early protein 1
    • IE1p72
    • IE72

Gene names

    • Name
      UL123

Organism names

Accessions

  • Primary accession
    P13202
  • Secondary accessions
    • Q7M6S4

Proteomes

Subcellular Location

Host nucleus
Note: Colocalizes with host PML-associated nuclear bodies very early post infection.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis174Enhanced degradation of IE1 during infection; complete loss of interference with PML sumoylation, disruption of PML-associated nuclear bodies and transactivation activity.

PTM/Processing

Features

Showing features for chain, cross-link.

Type
IDPosition(s)Description
ChainPRO_00001153551-491Immediate early protein IE1
Cross-link450Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modification

Sumoylated by host PML (PubMed:28250117).
Sumoylation abolishes the interaction with host STAT2 and thus the IE1-mediated repression of interferon-stimulated genes (By similarity).

Keywords

Expression

Keywords

Interaction

Subunit

Forms homodimers (PubMed:34370791).
Interacts with human p53/TP53; this interaction inhibits p53/TP53-dependent transactivation activity. Interacts with host STAT1. Interacts with host STAT2; this interaction promotes viral growth and counteracts the antiviral interferon response. May also interact with the host STAT1-STAT2 heterodimer. Interacts with host STAT3; this interaction leads to STAT3 nuclear accumulation and disruption of IL6-induced STAT3 phosphorylation (By similarity).
Interacts with host PML; this interaction inhibits host PML de novo sumoylation and probably inhibits PML regulation of type I and type II interferon-induced gene expression (PubMed:27903803, PubMed:34370791).
Interacts with host DAXX. Interacts with host SP100. Interacts with host E2F1. Interacts with host RB1. Interacts with host HDAC1; this interaction inhibits histone deacetylation and promotes viral transcription. Interacts with host HDAC2; this interaction inhibits histone deacetylation and promotes viral transcription. Interacts with host HDAC3; this interaction inhibits histone deacetylation and promotes viral transcription (By similarity).
Interacts with host PLSCR1; this interaction inhibits IE1 transactivating activity

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for compositional bias, region.

Type
IDPosition(s)Description
Compositional bias1-19Basic and acidic residues
Region1-24Nuclear localization signal
Region1-30Disordered
Region132-346Interaction with host PML, interference with PML sumoylation and disruption of PML-associated nuclear bodies
Region373-445Interaction with host STAT2
Region410-420Modulation of STAT3/STAT1 signaling
Region410-445Interaction with host STAT3
Region421-472Acidic
Region421-491Disordered
Compositional bias425-442Acidic residues
Region449-452Interaction with host SUMO1
Region475-491Chromosome-tethering domain (CTD), binding to histones

Domain

The N-terminal region is required for nuclear targeting. The C-terminal 16-amino acid is termed the chromosome-tethering domain (CTD) and is required for the association of IE1, host PML and host STAT2 with the mitotic chromosomes. Targets host nucleosomes by directly binding to the acidic pocket of core histones.

Sequence similarities

Belongs to the HHV-5 IE1 protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    491
  • Mass (Da)
    55,110
  • Last updated
    1990-01-01 v1
  • Checksum
    CC5966B00CD8C8B4
MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEVNSQLSLGDPLFPELAEESLKTFEQVTEDCNENPEKDVLAELVKQIKVRVDMVRHRIKEHMLKKYTQTEEKFTGAFNMMGGCLQNALDILDKVHEPFEEMKCIGLTMQSMYENYIVPEDKREMWMACIKELHDVSKGAANKLGGALQAKARAKKDELRRKMMYMCYRNIEFFTKNSAFPKTTNGCSQAMAALQNLPQCSPDEIMAYAQKIFKILDEERDKVLTHIDHIFMDILTTCVETMCNEYKVTSDACMMTMYGGISLLSEFCRVLCCYVLEETSVMLAKRPLITKPEVISVMKRRIEEICMKVFAQYILGADPLRVCSPSVDDLRAIAEESDEEEAIVAYTLATAGVSSSDSLVSPPESPVPATIPLSSVIVAENSDQEESEQSDEEEEEGAQEEREDTVSVKSEPVSEIEEVAPEEEEDGAEEPTASGGKSTHPMVTRSKADQ

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-19Basic and acidic residues
Compositional bias425-442Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X17403
EMBL· GenBank· DDBJ
CAA35325.1
EMBL· GenBank· DDBJ
Genomic DNA
M21295
EMBL· GenBank· DDBJ
AAA45980.1
EMBL· GenBank· DDBJ
Genomic DNA
BK000394
EMBL· GenBank· DDBJ
DAA00112.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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