P13065 · PHSL_DESBA
- ProteinPeriplasmic [NiFeSe] hydrogenase large subunit
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids514 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
Miscellaneous
Perhaps the leader of the small subunit serves as a transport vehicle for both subunits.
Catalytic activity
- H2 + A = AH2
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 irons ions. Iron 1 has 3 cyanide and carbon monoxide ligands. Iron 2 has 3 water ligands.
Note: Binds 1 nickel ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 52 | Fe cation 2 (UniProtKB | ChEBI) | |||
Binding site | 71 | Ni2+ (UniProtKB | ChEBI) | |||
Binding site | 74 | Fe cation 1 (UniProtKB | ChEBI) | |||
Binding site | 74 | Ni2+ (UniProtKB | ChEBI) | |||
Binding site | 445 | Fe cation 2 (UniProtKB | ChEBI) | |||
Binding site | 493 | Ni2+ (UniProtKB | ChEBI) | |||
Binding site | 496 | Fe cation 1 (UniProtKB | ChEBI) | |||
Binding site | 496 | Ni2+ (UniProtKB | ChEBI) | |||
Binding site | 499 | Fe cation 2 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | periplasmic space | |
Molecular Function | ferredoxin hydrogenase activity | |
Molecular Function | hydrogenase (acceptor) activity | |
Molecular Function | nickel cation binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeriplasmic [NiFeSe] hydrogenase large subunit
- EC number
- Alternative names
Organism names
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfovibrionia > Desulfovibrionales > Desulfomicrobiaceae > Desulfomicrobium
Accessions
- Primary accessionP13065
Subcellular Location
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Initiator methionine | 1 | Removed | |||
Chain | PRO_0000199704 | 2-514 | Periplasmic [NiFeSe] hydrogenase large subunit | ||
Structure
Sequence
- Sequence statusComplete
- Length514
- Mass (Da)56,862
- Last updated2008-02-26 v3
- ChecksumAC920132081C1ADC
Features
Showing features for non-standard residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Non-standard residue | 493 | Selenocysteine | |||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M18271 EMBL· GenBank· DDBJ | AAA23375.2 EMBL· GenBank· DDBJ | Genomic DNA |