P12930 · CAPSD_PAVCN
- ProteinCapsid protein VP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids727 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids (By similarity).
Miscellaneous
The capsids of autonomous parvoviruses expose a proportion of VP2 N-terminus and part of that sequence can be cleaved of to form VP3.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | host cell | |
Cellular Component | host cell nucleus | |
Cellular Component | T=1 icosahedral viral capsid | |
Molecular Function | metal ion binding | |
Molecular Function | structural molecule activity | |
Biological Process | adhesion receptor-mediated virion attachment to host cell | |
Biological Process | clathrin-dependent endocytosis of virus by host cell | |
Biological Process | entry receptor-mediated virion attachment to host cell | |
Biological Process | microtubule-dependent intracellular transport of viral material towards nucleus | |
Biological Process | symbiont entry into host cell via permeabilization of host membrane | |
Biological Process | viral penetration into host nucleus |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameCapsid protein VP1
- Alternative names
Organism names
- Taxonomic lineageViruses > Monodnaviria > Shotokuvirae > Cossaviricota > Quintoviricetes > Piccovirales > Parvoviridae > Parvovirinae > Protoparvovirus > Protoparvovirus carnivoran1
- Virus hosts
Accessions
- Primary accessionP12930
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000039429 | 1-727 | Capsid protein VP1 | |||
Sequence: MAPPAKRARRGLVPPGYKYLGPGNSLDQGEPTNPSDAAAKEHDEAYAAYLRSGKNPYLYFSPADQRFIDQTKDAKDWGGKIGHYFFRAKKAIAPVLTDTPDHPSTSRPTKPTKRSKPPPHIFINLAKKKKAGAGQVKRDNLAPMSDGAVQPDGGQPAVRNERATGSGNGSGGGGGGGSGGVGISTGTFNNQTEFKFLENGWVEITANSSRLVHLNMPESENYRRVVVNNMDKTAVNGNMALDDIHAQIVTPWSLVDANAWGVWFNPGDWQLIVNTMSELHLVSFEQEIFNVVLKTVSESATQPPTKVYNNDLTASLMVALDSNNTMPFTPAAMRSETLGFYPWKPTIPTPWRYYFQWDRTLIPSHTGTSGTPTNIYHGTDPDDVQFYTIENSVPVHLLRTGDEFATGTFFFDCKPCRLTHTWQTNRALGLPPFLNSLPQSEGATNFGDIGVQQDKRRGVTQMGNTNYITEATIMRPAEVGYSAPYYSFEASTQGPFKTPIAAGRGGAQTYENQAADGDPRYAFGRQHGQKTTTTGETPERFTYIAHQDTGRYPEGDWIQNINFNLPVTNDNVLLPTDPIGGKTGINYTNIFNTYGPLTALNNVPPVYPNGQIWDKEFDTDLKPRLHVNAPFVCQNNCPGQLFVKVAPNLTNEYDPDASANMSRIVTYSDFWWKGKLVFKAKLRASHTWNPIQQMSINVDNQFNYVPSNIGGMKIVYEKSQLAPRKLY | ||||||
Disulfide bond | 633↔637 | |||||
Sequence: CQNNC |
Keywords
- PTM
Interaction
Subunit
Interacts with host TFRC.
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-39 | Disordered | ||||
Sequence: MAPPAKRARRGLVPPGYKYLGPGNSLDQGEPTNPSDAAA | ||||||
Motif | 4-13 | Nuclear localization signal | ||||
Sequence: PAKRARRGLV | ||||||
Region | 19-64 | Phospholipase A2-like | ||||
Sequence: YLGPGNSLDQGEPTNPSDAAAKEHDEAYAAYLRSGKNPYLYFSPAD | ||||||
Region | 95-120 | Disordered | ||||
Sequence: VLTDTPDHPSTSRPTKPTKRSKPPPH | ||||||
Region | 141-184 | Disordered | ||||
Sequence: LAPMSDGAVQPDGGQPAVRNERATGSGNGSGGGGGGGSGGVGIS |
Domain
The N-terminus of VP1 is sequestered within the mature capsid. It contains a phospholipase A2-like region and nuclear localization signals that might be exposed by capsid modifications during virus entry (By similarity).
Sequence similarities
Belongs to the parvoviridae capsid protein family.
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
P12930-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameVP1
- NoteMinor splicing isoform.
- Length727
- Mass (Da)80,392
- Last updated2011-05-31 v2
- Checksum76EA7954CC4539FB
P12930-2
- NameVP2
- NoteMajor splicing isoform produced by deletion of the initiating AUG for VP1 and downstream translation of VP2.
- Differences from canonical
- 1-143: Missing
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_041139 | 1-143 | in isoform VP2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M19296 EMBL· GenBank· DDBJ | AAA67460.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
M19296 EMBL· GenBank· DDBJ | AAA67461.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. |