P12758 · UDP_ECOLI
- ProteinUridine phosphorylase
- Geneudp
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids253 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the reversible phosphorylytic cleavage of uridine to uracil and ribose-1-phosphate (PubMed:16751). Shows weak activity towards deoxyuridine and thymidine (PubMed:16751). The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (Probable).
Catalytic activity
- phosphate + uridine = alpha-D-ribose 1-phosphate + uracil
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.15 mM | uridine | |||||
7.3 mM | phosphate | |||||
0.24 mM | uracyl | |||||
1.4 mM | ribose-1-phosphate |
pH Dependence
Optimum pH is 7.3.
Pathway
Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | protein-containing complex | |
Molecular Function | ATP binding | |
Molecular Function | identical protein binding | |
Molecular Function | potassium ion binding | |
Molecular Function | uridine phosphorylase activity | |
Biological Process | DNA damage response | |
Biological Process | UMP catabolic process | |
Biological Process | UMP salvage | |
Biological Process | uridine catabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUridine phosphorylase
- EC number
- Short namesUPase ; UrdPase
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP12758
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 5 | No change in activity. | ||||
Sequence: D → A, E, or N |
Chemistry
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000063183 | 2-253 | Uridine phosphorylase | |||
Sequence: SKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKLASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHINVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSDTFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAGVIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length253
- Mass (Da)27,159
- Last updated2007-01-23 v3
- Checksum7446DE45BA04D88D
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 11 | in Ref. 7; AA sequence | ||||
Sequence: L → Y | ||||||
Sequence conflict | 15 | in Ref. 7; AA sequence | ||||
Sequence: D → Y |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X15689 EMBL· GenBank· DDBJ | CAA33724.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M87049 EMBL· GenBank· DDBJ | AAA67626.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC76834.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE77470.1 EMBL· GenBank· DDBJ | Genomic DNA |