P12758 · UDP_ECOLI

Function

function

Catalyzes the reversible phosphorylytic cleavage of uridine to uracil and ribose-1-phosphate (PubMed:16751). Shows weak activity towards deoxyuridine and thymidine (PubMed:16751). The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis (Probable).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.15 mMuridine
7.3 mMphosphate
0.24 mMuracyl
1.4 mMribose-1-phosphate

pH Dependence

Optimum pH is 7.3.

Pathway

Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentprotein-containing complex
Molecular FunctionATP binding
Molecular Functionidentical protein binding
Molecular Functionpotassium ion binding
Molecular Functionuridine phosphorylase activity
Biological ProcessDNA damage response
Biological ProcessUMP catabolic process
Biological ProcessUMP salvage
Biological Processuridine catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Uridine phosphorylase
  • EC number
  • Short names
    UPase
    ; UrdPase

Gene names

    • Name
      udp
    • Ordered locus names
      b3831, JW3808

Organism names

  • Taxonomic identifier
  • Strains
    • K12
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P12758
  • Secondary accessions
    • Q2M8D6

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis5No change in activity.

Chemistry

    • DB074391-((2-HYDROXYETHOXY)METHYL)-5-(3-(BENZYLOXY)BENZYL)-6-HYDROXYPYRIMIDINE-2,4(1H,3H)-DIONE
    • DB068731-((2-HYDROXYETHOXY)METHYL)-5-(3-(BENZYLOXY)BENZYL)PYRIMIDINE-2,4(1H,3H)-DIONE
    • DB068721-((2-HYDROXYETHOXY)METHYL)-5-(PHENYLTHIO)PYRIMIDINE-2,4(1H,3H)-DIONE
    • DB022562'-Deoxyuridine
    • DB026812,8-bis[oxido(oxo)vanadio]-1,1,1,3,5,5,7,7,9,9,9-undecaoxopentavanadoxane-2,8-diium

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000631832-253Uridine phosphorylase

Proteomic databases

Interaction

Subunit

Homohexamer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the PNP/UDP phosphorylase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    253
  • Mass (Da)
    27,159
  • Last updated
    2007-01-23 v3
  • Checksum
    7446DE45BA04D88D
MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKLASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHINVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSDTFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAGVIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict11in Ref. 7; AA sequence
Sequence conflict15in Ref. 7; AA sequence

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X15689
EMBL· GenBank· DDBJ
CAA33724.1
EMBL· GenBank· DDBJ
Genomic DNA
M87049
EMBL· GenBank· DDBJ
AAA67626.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC76834.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE77470.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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