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P12311 · ADH1_GEOSE

Function

function

NAD+-dependent alcohol dehydrogenase.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per subunit.

Activity regulation

Substrate inhibition is not observed with any alcohols, and the enzyme-NADH dissociation is not considered to be a rate-limiting step.

Temperature Dependence

Thermostable.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site38Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site61Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site92Zn2+ 2 (UniProtKB | ChEBI)
Binding site95Zn2+ 2 (UniProtKB | ChEBI)
Binding site98Zn2+ 2 (UniProtKB | ChEBI)
Binding site106Zn2+ 2 (UniProtKB | ChEBI)
Binding site148Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site172-177NAD+ (UniProtKB | ChEBI)
Binding site195NAD+ (UniProtKB | ChEBI)
Binding site200NAD+ (UniProtKB | ChEBI)
Binding site260-262NAD+ (UniProtKB | ChEBI)
Binding site331NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionalcohol dehydrogenase (NAD+) activity
Molecular Functionzinc ion binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alcohol dehydrogenase
  • EC number
  • Alternative names
    • ADH-T

Gene names

    • Name
      adhT

Organism names

Accessions

  • Primary accession
    P12311

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis38No activity.
Mutagenesis40No activity.
Mutagenesis40Little decrease in activity.
Mutagenesis43No activity.
Mutagenesis43Higher level of activity at pH 9.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001607361-337Alcohol dehydrogenase

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    337
  • Mass (Da)
    36,100
  • Last updated
    1995-11-01 v2
  • MD5 Checksum
    FCA46199FF817A0C41BD08190D196D5C
MKAAVVEQFKKPLQVKEVEKPKISYGEVLVRIKACGVCHTDLHAAHGDWPVKPKLPLIPGHEGVGVIEEVGPGVTHLKVGDRVGIPWLYSACGHCDYCLSGQETLCERQQNAGYSVDGGYAEYCRAAADYVVKIPDNLSFEEAAPIFCAGVTTYKALKVTGAKPGEWVAIYGIGGLGHVAVQYAKAMGLNVVAVDLGDEKLELAKQLGADLVVNPKHDDAAQWIKEKVGGVHATVVTAVSKAAFESAYKSIRRGGACVLVGLPPEEIPIPIFDTVLNGVKIIGSIVGTRKDLQEALQFAAEGKVKTIVEVQPLENINDVFDRMLKGQINGRVVLKVD

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict22in Ref. 2; AA sequence
Sequence conflict33in Ref. 2; AA sequence
Sequence conflict52-53in Ref. 3; AA sequence

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D90421
EMBL· GenBank· DDBJ
BAA14411.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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