P12311 · ADH1_GEOSE
- ProteinAlcohol dehydrogenase
- GeneadhT
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids337 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
NAD+-dependent alcohol dehydrogenase.
Catalytic activity
- a primary alcohol + NAD+ = an aldehyde + NADH + H+
Cofactor
Note: Binds 2 Zn2+ ions per subunit.
Activity regulation
Substrate inhibition is not observed with any alcohols, and the enzyme-NADH dissociation is not considered to be a rate-limiting step.
Temperature Dependence
Thermostable.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 38 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | |||
Binding site | 61 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | |||
Binding site | 92 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 95 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 98 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 106 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 148 | Zn2+ 1 (UniProtKB | ChEBI); catalytic | |||
Binding site | 172-177 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 195 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 200 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 260-262 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 331 | NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | alcohol dehydrogenase (NAD+) activity | |
Molecular Function | zinc ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlcohol dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Geobacillus
Accessions
- Primary accessionP12311
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 38 | No activity. | |||
Mutagenesis | 40 | No activity. | |||
Mutagenesis | 40 | Little decrease in activity. | |||
Mutagenesis | 43 | No activity. | |||
Mutagenesis | 43 | Higher level of activity at pH 9. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000160736 | 1-337 | Alcohol dehydrogenase | ||
Structure
Sequence
- Sequence statusComplete
- Length337
- Mass (Da)36,100
- Last updated1995-11-01 v2
- MD5 ChecksumFCA46199FF817A0C41BD08190D196D5C
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 22 | in Ref. 2; AA sequence | |||
Sequence conflict | 33 | in Ref. 2; AA sequence | |||
Sequence conflict | 52-53 | in Ref. 3; AA sequence | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D90421 EMBL· GenBank· DDBJ | BAA14411.1 EMBL· GenBank· DDBJ | Genomic DNA |