P11633 · NHP6B_YEAST
- ProteinNon-histone chromosomal protein 6B
- GeneNHP6B
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
DNA-binding protein that induces severe bending of DNA. Required for DNA-binding by the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. Also augments the fidelity of transcription by RNA polymerase III independently of any role in the FACT complex. Required for transcriptional initiation fidelity of some but not all tRNA genes. Seems to be functionally redundant with NHP6A.
Miscellaneous
Present with 4590 molecules/cell in log phase SD medium.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 27-95 | HMG box | ||||
Sequence: PKRGLSAYMFFANENRDIVRSENPDVTFGQVGRILGERWKALTAEEKQPYESKAQADKKRYESEKELYN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | nucleus | |
Molecular Function | DNA binding, bending | |
Molecular Function | sequence-specific DNA binding | |
Biological Process | chromatin remodeling | |
Biological Process | DNA repair | |
Biological Process | maintenance of transcriptional fidelity during transcription elongation by RNA polymerase III | |
Biological Process | RNA polymerase III preinitiation complex assembly | |
Biological Process | transcription by RNA polymerase II |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNon-histone chromosomal protein 6B
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP11633
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000048566 | 1-99 | Non-histone chromosomal protein 6B | |||
Sequence: MAATKEAKQPKEPKKRTTRRKKDPNAPKRGLSAYMFFANENRDIVRSENPDVTFGQVGRILGERWKALTAEEKQPYESKAQADKKRYESEKELYNATRA |
Proteomic databases
PTM databases
Interaction
Subunit
Weakly associates with the stable SPT16-POB3 heterodimer to form the FACT (yFACT or SNP) complex, which is associated with nucleosomes. Multiple molecules of NHP6 (NHP6A and/or NHP6B) are required to recruit the SPT16-POB3 heterodimer to DNA.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-31 | Disordered | ||||
Sequence: MAATKEAKQPKEPKKRTTRRKKDPNAPKRGL | ||||||
Region | 69-99 | Disordered | ||||
Sequence: TAEEKQPYESKAQADKKRYESEKELYNATRA |
Sequence similarities
Belongs to the NHP6 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length99
- Mass (Da)11,476
- Last updated2011-07-27 v3
- Checksum0243FDC7D19B8A59
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 30 | in Ref. 2; X78993 and 3; CAA85042 | ||||
Sequence: G → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X15318 EMBL· GenBank· DDBJ | CAA33378.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X78993 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
Z35957 EMBL· GenBank· DDBJ | CAA85040.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z35959 EMBL· GenBank· DDBJ | CAA85042.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY558566 EMBL· GenBank· DDBJ | AAS56892.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
EF123125 EMBL· GenBank· DDBJ | ABM97469.1 EMBL· GenBank· DDBJ | mRNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07210.2 EMBL· GenBank· DDBJ | Genomic DNA |