P11491 · PPB_YEAST
- ProteinRepressible alkaline phosphatase
- GenePHO8
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids566 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Phosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate. Acts as a fructose-2,6-bisphosphate 6-phosphatase (PubMed:1848184).
Miscellaneous
Present with 3060 molecules/cell in log phase SD medium.
Catalytic activity
- a phosphate monoester + H2O = an alcohol + phosphate
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion.
Note: Binds 2 Zn2+ ions.
pH Dependence
Optimum pH is 7.0 for farnesyl diphosphatase activity.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 75 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 75 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 123 | Phosphoserine intermediate | ||||
Sequence: S | ||||||
Binding site | 174 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 176 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 325 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 330 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 334 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 373 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 374 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 484 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | fungal-type vacuole membrane | |
Molecular Function | alkaline phosphatase activity | |
Molecular Function | fructose-2,6-bisphosphate 6-phosphatase activity | |
Molecular Function | metal ion binding | |
Biological Process | nicotinamide nucleotide metabolic process | |
Biological Process | nucleotide catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRepressible alkaline phosphatase
- EC number
- Alternative names
- Cleaved into 1 chains
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP11491
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Repressible alkaline phosphatase
Vacuole membrane ; Single-pass membrane protein
Note: The full-length version is found in lysosome-like vacuoles.
Soluble alkaline phosphatase
Note: The truncated version of the protein is soluble.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-33 | Cytoplasmic | ||||
Sequence: MMTHTLPSEQTRLVPGSDSSSRPKKRRISKRSK | ||||||
Transmembrane | 34-59 | Helical | ||||
Sequence: IIVSTVVCIGLLLVLVQLAFPSSFAL | ||||||
Topological domain | 60-? | Vacuolar | ||||
Sequence: MMTHTLPSEQTRLVPGSDSSSRPKKRRISKRSKIIVSTVVCIGLLLVLVQLAFPSSFAL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for propeptide, chain, modified residue, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Propeptide | PRO_0000024018 | ?-566 | Removed in mature form | |||
Sequence: MMTHTLPSEQTRLVPGSDSSSRPKKRRISKRSKIIVSTVVCIGLLLVLVQLAFPSSFALRSASHKKKNVIFFVTDGMGPASLSMARSFNQHVNDLPIDDILTLDEHFIGSSRTRSSDSLVTDSAAGATAFACALKSYNGAIGVDPHHRPCGTVLEAAKLAGYLTGLVVTTRITDATPASFSSHVDYRWQEDLIATHQLGEYPLGRVVDLLMGGGRSHFYPQGEKASPYGHHGARKDGRDLIDEAQSNGWQYVGDRKNFDSLLKSHGENVTLPFLGLFADNDIPFEIDRDEKEYPSLKEQVKVALGALEKASNEDKDSNGFFLMVEGSRIDHAGHQNDPASQVREVLAFDEAFQYVLEFAENSDTETVLVSTSDHETGGLVTSRQVTASYPQYVWYPQVLANATHSGEFLKRKLVDFVHEHKGASSKIENFIKHEILEKDLGIYDYTDSDLETLIHLDDNANAIQDKLNDMVSFRAQIGWTTHGHSAVDVNIYAYANKKATWSYVLNNLQGNHENTEVGQFLENFLELNLNEVTDLIRDTKHTSDFDATEIASEVQHYDEYYHELTN | ||||||
Chain | PRO_0000024017 | 1-? | Repressible alkaline phosphatase | |||
Chain | PRO_0000401198 | 63-? | Soluble alkaline phosphatase | |||
Sequence: MMTHTLPSEQTRLVPGSDSSSRPKKRRISKRSKIIVSTVVCIGLLLVLVQLAFPSSFALRSA | ||||||
Modified residue | 123 | Phosphoserine | ||||
Sequence: S | ||||||
Glycosylation | 268 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 401 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-18 | Polar residues | ||||
Sequence: MMTHTLPSEQTRLVPGSD | ||||||
Region | 1-27 | Disordered | ||||
Sequence: MMTHTLPSEQTRLVPGSDSSSRPKKRR |
Sequence similarities
Belongs to the alkaline phosphatase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length566
- Mass (Da)63,004
- Last updated1998-07-15 v2
- Checksum9FA2E87B068FF0DB
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-18 | Polar residues | ||||
Sequence: MMTHTLPSEQTRLVPGSD | ||||||
Sequence conflict | 5 | in Ref. 1; AAA34871 | ||||
Sequence: T → R | ||||||
Sequence conflict | 55 | in Ref. 1; AAA34871 | ||||
Sequence: S → T | ||||||
Sequence conflict | 59 | in Ref. 1; AAA34871 | ||||
Sequence: L → I | ||||||
Sequence conflict | 132 | in Ref. 1; AAA34871 | ||||
Sequence: C → S | ||||||
Sequence conflict | 271 | in Ref. 1; AAA34871 | ||||
Sequence: L → F | ||||||
Sequence conflict | 328 | in Ref. 4; AAU09711 | ||||
Sequence: R → G | ||||||
Sequence conflict | 447 | in Ref. 1; AAA34871 | ||||
Sequence: D → E |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M21134 EMBL· GenBank· DDBJ | AAA34871.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U33050 EMBL· GenBank· DDBJ | AAB64930.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY723794 EMBL· GenBank· DDBJ | AAU09711.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006938 EMBL· GenBank· DDBJ | DAA12314.1 EMBL· GenBank· DDBJ | Genomic DNA |