P11491 · PPB_YEAST

Function

function

Phosphatase with broad substrate specificity. A truncated (soluble) version of the protein is responsible for the production of (E,E)-farnesol from (E,E)-farnesyl diphosphate. Acts as a fructose-2,6-bisphosphate 6-phosphatase (PubMed:1848184).

Miscellaneous

Present with 3060 molecules/cell in log phase SD medium.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions.

pH Dependence

Optimum pH is 7.0 for farnesyl diphosphatase activity.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site75Mg2+ (UniProtKB | ChEBI)
Binding site75Zn2+ 2 (UniProtKB | ChEBI)
Active site123Phosphoserine intermediate
Binding site174Mg2+ (UniProtKB | ChEBI)
Binding site176Mg2+ (UniProtKB | ChEBI)
Binding site325Mg2+ (UniProtKB | ChEBI)
Binding site330Zn2+ 1 (UniProtKB | ChEBI)
Binding site334Zn2+ 1 (UniProtKB | ChEBI)
Binding site373Zn2+ 2 (UniProtKB | ChEBI)
Binding site374Zn2+ 2 (UniProtKB | ChEBI)
Binding site484Zn2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentfungal-type vacuole membrane
Molecular Functionalkaline phosphatase activity
Molecular Functionfructose-2,6-bisphosphate 6-phosphatase activity
Molecular Functionmetal ion binding
Biological Processnicotinamide nucleotide metabolic process
Biological Processnucleotide catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      PHO8
    • ORF names
      D8035.24
    • Ordered locus names
      YDR481C

Organism names

Accessions

  • Primary accession
    P11491
  • Secondary accessions
    • D6VTA4
    • E9P949
    • Q03374

Proteomes

Organism-specific databases

Subcellular Location

Repressible alkaline phosphatase

Vacuole membrane ; Single-pass membrane protein
Note: The full-length version is found in lysosome-like vacuoles.

Soluble alkaline phosphatase

Note: The truncated version of the protein is soluble.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-33Cytoplasmic
Transmembrane34-59Helical
Topological domain60-?Vacuolar

Keywords

PTM/Processing

Features

Showing features for propeptide, chain, modified residue, glycosylation.

TypeIDPosition(s)Description
PropeptidePRO_0000024018?-566Removed in mature form
ChainPRO_00000240171-?Repressible alkaline phosphatase
ChainPRO_000040119863-?Soluble alkaline phosphatase
Modified residue123Phosphoserine
Glycosylation268N-linked (GlcNAc...) asparagine
Glycosylation401N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Interaction

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias1-18Polar residues
Region1-27Disordered

Sequence similarities

Belongs to the alkaline phosphatase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    566
  • Mass (Da)
    63,004
  • Last updated
    1998-07-15 v2
  • Checksum
    9FA2E87B068FF0DB
MMTHTLPSEQTRLVPGSDSSSRPKKRRISKRSKIIVSTVVCIGLLLVLVQLAFPSSFALRSASHKKKNVIFFVTDGMGPASLSMARSFNQHVNDLPIDDILTLDEHFIGSSRTRSSDSLVTDSAAGATAFACALKSYNGAIGVDPHHRPCGTVLEAAKLAGYLTGLVVTTRITDATPASFSSHVDYRWQEDLIATHQLGEYPLGRVVDLLMGGGRSHFYPQGEKASPYGHHGARKDGRDLIDEAQSNGWQYVGDRKNFDSLLKSHGENVTLPFLGLFADNDIPFEIDRDEKEYPSLKEQVKVALGALEKASNEDKDSNGFFLMVEGSRIDHAGHQNDPASQVREVLAFDEAFQYVLEFAENSDTETVLVSTSDHETGGLVTSRQVTASYPQYVWYPQVLANATHSGEFLKRKLVDFVHEHKGASSKIENFIKHEILEKDLGIYDYTDSDLETLIHLDDNANAIQDKLNDMVSFRAQIGWTTHGHSAVDVNIYAYANKKATWSYVLNNLQGNHENTEVGQFLENFLELNLNEVTDLIRDTKHTSDFDATEIASEVQHYDEYYHELTN

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias1-18Polar residues
Sequence conflict5in Ref. 1; AAA34871
Sequence conflict55in Ref. 1; AAA34871
Sequence conflict59in Ref. 1; AAA34871
Sequence conflict132in Ref. 1; AAA34871
Sequence conflict271in Ref. 1; AAA34871
Sequence conflict328in Ref. 4; AAU09711
Sequence conflict447in Ref. 1; AAA34871

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M21134
EMBL· GenBank· DDBJ
AAA34871.1
EMBL· GenBank· DDBJ
Genomic DNA
U33050
EMBL· GenBank· DDBJ
AAB64930.1
EMBL· GenBank· DDBJ
Genomic DNA
AY723794
EMBL· GenBank· DDBJ
AAU09711.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006938
EMBL· GenBank· DDBJ
DAA12314.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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